人参皂苷Rg1通过过氧化物酶体增殖物激活受体γ调控脂多糖诱导的炎症反应中BV2小胶质细胞的极化

李婷钰; 包翠芬; 王兴航; 迟晓晨; 李昆芳

doi:10.16098/j.issn.0529-1356.2023.03.003

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解剖学报 ›› 2023, Vol. 54 ›› Issue (3) : 269-275. DOI: 10.16098/j.issn.0529-1356.2023.03.003

神经生物学

人参皂苷Rg1通过过氧化物酶体增殖物激活受体γ调控脂多糖诱导的炎症反应中BV2小胶质细胞的极化

李婷钰¹ 王兴航¹ 迟晓晨¹ 李昆芳¹包翠芬^2*

作者信息 +

Ginsenoside Rg1 regulating BV2 microglia polarization in lipopolysaccharide-induced inflammatory response via peroxisome proliferator activated receptor γ

LI Ting-yu¹ WANG Xing-hang¹ CHI Xiao-chen¹ LI Kun-fang¹ BAO Cui-fen^2*#br#

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文章历史 +

摘要

目的通过脂多糖刺激BV2小胶质细胞建立炎症模型，探讨人参皂苷Rg1是否通过激活过氧化物酶体增殖物激活受体γ（PPARγ）受体蛋白对炎症的调控作用。方法 BV2小胶质细胞随机分为对照组、模型组、人参皂苷Rg1组、罗格列酮组、GW9662组。对照组不做任何处理，模型组加入1mg/L脂多糖，其他组在加入脂多糖处理后，再分别加入0.4mmol/L人参皂苷Rg1、10 μmol/L罗格列酮或10 μmol/L GW9662。CCK-8法检测各组BV2小胶质细胞增殖情况；采用免疫荧光和免疫印迹法检测PPARγ、磷酸化核因子κBp65(p-NF-κB p65）、诱导型一氧化氮合酶（iNOS)和人精氨酸酶1（ARG-1）蛋白的表达。ELISA法检测炎症因子白细胞介素1β（IL-1β）、白细胞介素6（IL-6）、白细胞介素8（IL-8）和肿瘤坏死因子α（TNF-α）的含量。结果与对照组相比，模型组细胞增殖率明显上升，IL-1β、IL-6、IL-8和TNF-α炎症因子含量明显增高，免疫荧光和免疫印迹结果显示，iNOS和p-NF-κB p65阳性表达明显增多，PPARγ和ARG-1阳性表达明显减少（均P＜0.01）；与模型组相比，人参皂苷Rg1组细胞增殖率降低，IL-1β、IL-6、IL-8和TNF-α表达水平降低，iNOS和p-NF-κB p65阳性表达明显减弱，PPARγ和ARG-1阳性表达明显增强（均P＜0.01）。结论人参皂苷Rg1抑制脂多糖刺激后BV2小胶质细胞的炎症反应，其机制可能与调控PPARγ/NF-κB通路从而促进小胶质细胞M2型极化有关。

Abstract

Objective To establish an inflammation model by stimulating BV2 microglia by lipopolysaccharide, and to explore the regulation effect of ginsenoside Rg1 on inflammation by activating peroxisome proliferator activated receptor γ(PPARγ) receptor protein. Methods BV2 microglia were randomly divided into control group, model group, ginsenoside Rg1 group, rosiglitazone group and GW9662 group. The control group did not do any treatment, the model group was treated with 1 mg/L lipopolysaccharide, and the other groups were treated with lipopolysaccharide added with 0.4mmol/L ginsenoside Rg1, 10 μmol/L rosiglitazone or 10 μmol/L respectively. GW9662. The proliferation of BV2 microglia in each group was detected by CCK-8 method; PPAR-γ, phospho-NF-κB p65 (p-NF-κB p65), induced expression of inducible nitric oxide synthase(iNOS) and human arginase 1(ARG-1) proteins. ELISA was used to detect the inflammatory factors interleukin-1β(IL-1β), interleukin-6(IL-6), interleukin-8(IL-8) and the content of tumor necrosis factor-α (TNF-α). Results Compared with the control group, the cell proliferation rate in the model group was significantly increased, and the contents of IL-1β, IL-6, IL-8 and TNF-α increased significantly. The results of immunofluorescence and Western blotting showed that iNOS and p-NF-κB p65 increased significantly, and the positive expressions of PPARγ and ARG-1 decreased significantly(both P＜0.01). The expression level of TNF-α decreased, the positive expressions of iNOS and p-NF-κB p65 decreased significantly, and the positive expressions of PPARγ and ARG-1 increased significantly(all P＜0.01). Conclusion Ginsenoside Rg1 inhibits the inflammatory response of BV2 microglia after lipopolysaccharide stimulation, and its mechanism may be related to the regulation of PPARγ/NF-κB pathway to promote the M2-type polarization of microglia.

导出引用

李婷钰王兴航迟晓晨李昆芳包翠芬. 人参皂苷Rg1通过过氧化物酶体增殖物激活受体γ调控脂多糖诱导的炎症反应中BV2小胶质细胞的极化[J]. 解剖学报. 2023, 54(3): 269-275 https://doi.org/10.16098/j.issn.0529-1356.2023.03.003

LI Ting-yu WANG Xing-hang CHI Xiao-chen LI Kun-fang BAO Cui-fen.

Ginsenoside Rg1 regulating BV2 microglia polarization in lipopolysaccharide-induced inflammatory response via peroxisome proliferator activated receptor γ

[J]. Acta Anatomica Sinica. 2023, 54(3): 269-275 https://doi.org/10.16098/j.issn.0529-1356.2023.03.003

中图分类号： R285

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