目的 了解大鼠肝再生(LR)0 h和6 h时骨髓瘤基因（MYC）及其mRNA相互作用的微小RNA（miRNA，miR）和环状RNA（circRNA）的表达变化、相互作用和调节残肝炎症反应的途径与方式。  方法 按Higgins等方法制备大鼠2/3肝切除（PH）模型，用大规模定量检测技术测定大鼠肝再生中残肝的mRNA、miRNA和circRNA［合称内源竞争RNA（ceRNA）］表达变化，用Cytoscape 3.2软件构建ceRNA的相互作用网，用ceRNA综合分析等方法解析它们的表达相关性和作用相关性。  结果 PH后0 h和 6 h时，MYC mRNA的比值为0.15±0.03和2.36±0.20，miR-540-3p为3.00±0.43和0.79±0.01，circRNA_04996为1.43±0.43和3.14±0.94。同时，PH后0 h时，MYC促进的纤溶酶原激活剂尿激酶受体（PLAUR）、肿瘤坏死因子（TNF）、白细胞介素1受体2型（IL1R2）等3个炎症反应相关基因表达下调，抑制的炎症反应相关基因利钠肽A（NPPA）、核受体亚家族O组B成员2（NROB2）和过氧化物酶体增殖物激活受体α（PPARA）表达上调。PH后6 h时，MYC促进的PLAUR、TNF、IL1R2等3个炎症反应相关基因表达上调，抑制的炎症反应相关基因NPPA、NROB2和PPARA表达下调。  结论 上述circRNA抑制的miRNA、miRNA抑制的MYC mRNA以及MYC调节的炎症反应相关基因的表达相关性和作用相关性有利于PH后0 h时残肝处于非炎症反应状态和PH后6 h时残肝处于炎症反应状态。

Objective To explore the role pathway and pattern of the transcription factor myeloma cancer gene (MYC) and its mRNA interaction with microRNA(miRNA, miR) and ciccular RNA(circRNAs) at 0 hour and 6 hour in the rat liver regeneration.   Methods The rat 2/3 hepatectomy (partial hepatectomy，PH) model was prepared as described by Higgins, the expression changes of mRNA, miRNA and circRNA ［together named as competing endogenous RNAs(ceRNA)］ of remnant liver were detected by the large-scale quantitative detection technology, the interaction network of ceRNA was constructed by Cytoscape 3.2 software, and their correlation in expression and role were analyzed by ceRNA comprehensive analysis.   Results It was found that at 0 hour and 6 hours after PH, the ratio value of MYC mRNA showed 0.15±0.03 and 2.36±0.20, miR-540-3p displays 3.00±0.43 and 0.79±0.01, circRNA_04996 showed 1.43±0.43 and 3.14±0.94. At the same time, the four kinds of inflammatory reaction-related genes plasminogen activator urokinase receptor (PLAUR), tumor necrosis factor (TNF), interleukin 1 receptor type 2 (IL1R2), ect, which were prometed in expression by MYC, were down-regulated at 0 hour after PH, but the inflammatory reaction-related genes natriuretic peptide A (NPPA), nuclear receptor subfamily O group B member 2 (NROB2) and peroxisome proliferator activated receptor alpha (PPARA), which were inhibited in expression by MYC, were up-regulated at 0 hour after PH. On the other hand, the three kinds of inflammatory reaction-related genes PLAUR, TNF, IL1R2, ect, which are prometed in expression by MYC, were up-regulated at 6 hours after PH, but the inflammatory reaction-related genes NPPA, NROB2 and PPARA, which were inhibited   in expression by MYC, were down-regulated at 6 hours after PH.   Conclusion The correlation in expression and role of the miRNA, which are inhibited by circRNAs, MYC, its mRNA is inhibited by miRNAs, and the inflammatory reaction-related genes, which are regulated by MYC, and are helpful for the hepatocyte to be in non-inflammatory reaction state at 0 hour after PH and to be in inflammatory reaction state at 6 hours after PH.