雌二醇对去卵巢大鼠抑郁行为的影响及其可能的作用机制

苏晓云; 贺继平; 崔建梅; 师先锋; 门杰

doi:10.16098/j.issn.0529-1356.2023.01.005

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解剖学报 ›› 2023, Vol. 54 ›› Issue (1) : 36-41. DOI: 10.16098/j.issn.0529-1356.2023.01.005

神经生物学

雌二醇对去卵巢大鼠抑郁行为的影响及其可能的作用机制

苏晓云¹ 贺继平^2* 崔建梅³ 师先锋⁴门杰²

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Effects of estradiol on depressive-like behavior of ovariectomized rats and its possible mechanism

SU Xiao-yun¹ HE Ji-ping^2* CUI Jian-mei³ SHI Xian-feng⁴ MEN Jie²

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摘要

目的通过观察雌二醇（E2）干预对去卵巢（OVX）大鼠杏仁核促炎因子白细胞介素6（IL-6）及肿瘤坏死因子α（TNF-α）水平和脑源性神经营养因子（BDNF）表达的影响，探讨E2改善OVX大鼠抑郁样行为的可能机制。方法将39只健康成年雌性SD大鼠随机分为3组，分别为假手术组（SO）、OVX组（OVX）及OVX+E2治疗组（OVX+E2）。E2治疗6周通过旷场实验（OFT）及糖水偏爱实验（SPT）评估大鼠抑郁样行为；ELISA法测定杏仁核IL-6及TNF-α水平，免疫组织化学染色法检测大鼠杏仁核中BDNF表达。结果 SPT实验结果显示，OVX使大鼠糖水摄入量及糖水偏好率均显著下降，而E2干预能够显著增加OVX大鼠的糖水摄入量及糖水偏好率；OFT结果显示，OVX使大鼠中央格时间、跨格次数及直立次数显著下降，E2干预能够显著增加OVX大鼠的中央格时间、跨格次数及直立次数；ELISA及免疫组织化学检测发现，与SO组比较，OVX大鼠杏仁核区促炎因子 IL-6 及 TNF-α 水平增加，BDNF 阳性细胞平均吸光度（AA）显著下降（均P <0.01）；与OVX组比较，OVX+E2组大鼠杏仁核区促炎因子 IL-6 及 TNF-α 水平下降（均P <0.01），BDNF 阳性细胞AA增加（P<0.05）。结论 E2干预可显著改善OVX大鼠的抑郁样行为，可能与E2增强OVX大鼠杏仁核抗炎能力和神经营养作用从而增强OVX大鼠的神经保护作用有关。

Abstract

Objective To examine the effect of estradiol (E2) treatment on ovariectomy (OVX) induced depressive-like behavior and possible mechanism by measuring inflammatory biomarkers levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) and brain-derived neurotrophic factor (BDNF) expression in amygdala nucleus. Methods Thirty nine healthy adult female SD rats were randomly divided into three groups: sham operation group (SO), ovariectomized group (OVX) and ovariectomized estradiol treatment group (OVX + E2). After 6 weeks of E2 treatment, depressive like behavior was evaluated by opening field test (OFT) and sugar water preference test (SPT). The levels of inflammatory factors IL-6 and TNF-α in amygdala were measured by ELESA, and the expression of BDNF in rat amygdala was detected by immunohistochemical staining. Results The result of the SPT showed that OVX significantly decreased the sugar intake and sugar preference rate of rats, and E2 treatment significantly increased sugar water intake and sugar water preference rate of rats. The result of the OFT showed that OVX significantly decreased the numbers of crossing and rearing of rats, and reduced the time spent in the centre; E2 treatment significantly increased the numbers of crossing and rearing of rats, and prolonged the time spent in the centre. ELESA and immunohistochemical analysis found the levels of IL-6 and TNF-α in amygdala increased significantly, while the average absorbance(AA) of BDNF in the amygdala reduced significantly (P<0.01 respectively) of rats in OVX group when compared with the SO group. And the levels of IL-6 and TNF-α in amygdala decreased significantly (P<0.01 respectively), while the AA of BDNF increased significantly (P<0.05) in the amygdala of rats in the OVX+EX group when compared with the OVX group. The difference was statisticaly significant. Conclusion E2 treatment improved depression-like behavior of OVX rats is partly due to increased anti-inflammatory and activated the BDNF expression in amygdaloid nucleus, thus enhancing the neuroprotective effect of OVX rats.

关键词

雌二醇 / 抑郁行为 / 杏仁核 / 炎症因子 / 脑源性神经营养因子 / 免疫组织化学 / 大鼠

Key words

Estradiol

/ Depressive behavior / Amygdaloid nucleus / Inflammatory factor / Brain-derived neurotrophic factor / Immunohistochemistry / Rat

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苏晓云贺继平崔建梅师先锋门杰. 雌二醇对去卵巢大鼠抑郁行为的影响及其可能的作用机制[J]. 解剖学报. 2023, 54(1): 36-41 https://doi.org/10.16098/j.issn.0529-1356.2023.01.005

SU Xiao-yun HE Ji-ping CUI Jian-mei SHI Xian-feng MEN Jie. Effects of estradiol on depressive-like behavior of ovariectomized rats and its possible mechanism[J]. Acta Anatomica Sinica. 2023, 54(1): 36-41 https://doi.org/10.16098/j.issn.0529-1356.2023.01.005

中图分类号： R74

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