石菖蒲挥发油对炎性痛大鼠脊髓背角中胶质纤维酸性蛋白、c-Jun氨基末端蛋白激酶和肿瘤坏死因子α表达的影响

张润恒; 杨翠珠; 李诗琪; 王姝涵; 王欣; 刘靖; 马宇昕

doi:10.16098/j.issn.0529-1356.2023.01.003

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解剖学报 ›› 2023, Vol. 54 ›› Issue (1) : 23-29. DOI: 10.16098/j.issn.0529-1356.2023.01.003

神经生物学

石菖蒲挥发油对炎性痛大鼠脊髓背角中胶质纤维酸性蛋白、c-Jun氨基末端蛋白激酶和肿瘤坏死因子α表达的影响

张润恒杨翠珠李诗琪王姝涵王欣刘靖马宇昕*

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Effects of volatile oil from Acori graminei Rhizoma on glial fibrillary acidic protein, c-Jun N-terminal protein kinase and tumor necrosis factor-α expressions in the spinal cord dorsal horn of the inflammatory pain rats

ZHANG Run-heng YANG Cui-zhu LI Shi-qi WANG Shu-han WANG Xin LIU Jing MA Yu-xin*

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摘要

目的探讨石菖蒲挥发油（VOA）对炎性痛大鼠脊髓背角中胶质纤维酸性蛋白（GFAP）、c-Jun氨基末端蛋白激酶（JNK）和肿瘤坏死因子α（TNF-α）表达的影响。方法 36只雄性SD大鼠随机分为：对照组（control）、假手术组（sham）、完全弗氏佐剂组（CFA）、5 g/（kg·d）低剂量VOA + CFA组（VOA-L+CFA）、10 g/（kg·d）中剂量VOA + CFA组（VOA-M+CFA)和20 g/（kg·d）高剂量VOA + CFA组（VOA-H+CFA)，共6组，每组6只，于连续灌胃给药的第22天取材。利用免疫荧光染色和Western blotting技术检测各组大鼠脊髓背角中GFAP、JNK和TNF-α的表达情况。结果免疫荧光染色及Western blotting结果表明，与control和sham组相比，CFA组中大鼠脊髓背角中GFAP、JNK和TNF-α阳性表达均显著增多（P<0.01）；与CFA组相比，不同剂量VOA处理组大鼠脊髓背角中GFAP、JNK和TNF-α阳性表达均减少，且VOA-H+CFA组大鼠脊髓背角中GFAP、JNK和TNF-α阳性表达减少更明显（P<0.05，P<0.01）。结论 VOA可降低CFA诱导的炎性痛大鼠脊髓背角中GFAP、JNK和TNF-α的表达。

Abstract

Objective To investigate the influence of volatile oil from Acori graminei Rhizoma（VOA）on expressions of glial fibrillary acidic protein (GFAP), c-Jun N-terminal protein kainse (JNK) and tumour necrosis factor-α (TNF-α) in the spinal cord dorsal horn of imflammatory pain rats. Methods Totally 36 male SD rats were randomly divided into control group (control), sham-operated group (sham), complete Freund’s adjuvant group (CFA), 5 g/(kg·d) low dose VOA+CFA group (VOA-L+CFA), 10 g/(kg·d) medium dose VOA + CFA group (VOA-M+CFA) and 20 g/(kg·d) high dose VOA + CFA group (VOA-H+CFA). All animals were sacrificed immediately after continuous gavage administration for 22 days. The expressions of GFAP, JNK and TNF-α in the spinal cord dorsal horn of rats in each group were detected by immunofluorescence and Western blotting methods. Results The present results showed that the positive expressions of GFAP, JNK and TNF-α in the spinal cord dorsal horn of rats increased significantly in the CFA group, when compared to the control and sham groups (P< 0.01). The expressions of GFAP, JNK and TNF-α in the spinal cord dorsal horn of rats with VOA treatment reduced in the dose-dependent manner, when compared to the CFA group, the positive expressions of GFAP, JNK and TNF-α reduced significantly in the dorsal horn of the spinal cord of the VOA-H+CFA group (P<0.05, P<0.01). Conclusion VOA reduces the expressions of GFAP, JNK and TNF-α in the spinal cord dorsal horn of rats of CFA-induced inflammatory pain.

关键词

石菖蒲挥发油 / 炎性痛 / 胶质纤维酸性蛋白 / c-Jun氨基末端蛋白激酶 / 肿瘤坏死因子α / 免疫荧光 / 免疫印迹法 / 大鼠

Key words

Volatile oil from Acori graminei Rhizoma

/ Inflammatory pain / Glial fibrillary acidic protein / c-Jun N-terminal kinase / Tumour necrosis factor-α / / Immunofluorescence / Western blotting / Rat

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张润恒杨翠珠李诗琪王姝涵王欣刘靖马宇昕. 石菖蒲挥发油对炎性痛大鼠脊髓背角中胶质纤维酸性蛋白、c-Jun氨基末端蛋白激酶和肿瘤坏死因子α表达的影响[J]. 解剖学报. 2023, 54(1): 23-29 https://doi.org/10.16098/j.issn.0529-1356.2023.01.003

ZHANG Run-heng YANG Cui-zhu LI Shi-qi WANG Shu-han WANG Xin LIU Jing MA Yu-xin.

[J]. Acta Anatomica Sinica. 2023, 54(1): 23-29 https://doi.org/10.16098/j.issn.0529-1356.2023.01.003

中图分类号： R338.2+2

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