目的 探讨微小RNA（miR）-128-3p对卵巢癌细胞上皮-间充质转化（EMT）的影响及其对锌指E盒同源结合蛋白1（ZEB1）的调控机制。  方法 通过Real-time PCR技术检测上皮性卵巢癌组织（EOC）及癌旁正常组织（各30例）中miR-128-3p的表达量并观察其是否存在差异；选取2种人卵巢癌细胞系SKOV3和A2780，分别转染miR-128-3p模拟物（miR-128-3p mimic）组和阴性对照模拟物（NC mimic）组，利用Real-time PCR技术检测4组miR-128-3p的表达量并验证干扰效果，用Transwell实验观察4组细胞的迁移及侵袭能力。通过双荧光素酶实验验证miR-128-3p与ZEB1的调控关系，用Western blotting检测过表达miR-128-3p后ZEB1蛋白的表达水平；在SKOV3和A2780细胞系中转染pcDNA-ZEB1使其过表达ZEB1，分为NC mimic组，miR-128-3p mimic组，miR-128-3p mimic+ pcDNA-ZEB1组，用Western blotting检测6组细胞中EMT相关蛋白E-钙黏蛋白(E-cadherin)及波形蛋白(vimentin)的表达水平。  结果 Real-time PCR结果显示，与癌旁组织相比，miR-128-3p在EOC组织中表达下降（P<0.01）；miR-128-3p mimic组miR-128-3p相对表达量高于NC mimic组，而迁移细胞数量、侵袭细胞数量低于NC mimic组（P<0.01）。 双荧光素酶实验结果显示，miR-128-3p对ZEB1存在负向调控作用。SKOV3、A2780细胞中miR-128-3p mimic组的ZEB1蛋白相对表达量均低于NC mimic组，而E-cadhein的蛋白相对表达量均高于NC mimic组（P<0.01）。MiR-128-3p mimic+ pcDNA-ZEB1组E-cadhein的蛋白相对表达量低于miR-128-3p mimic组（P<0.01）。SKOV3、A2780细胞中miR-128-3p mimic组vimentin的蛋白相对表达量低于NC mimic组，miR-128-3p mimic+ pcDNA-ZEB1组vimentin的蛋白相对表达量高于miR-128-3p mimic组（P<0.01）。  结论 MiR-128-3p在上皮性卵巢癌组织中表达下降，其原因可能是通过调控ZEB1影响EMT相关蛋白表达而参与卵巢癌细胞EMT过程。

Objective To investigate the effect of mircoRNA-128-3p (miR-128-3p) on epithelial-mesenchymal transition (EMT) of ovarian cancer cells and its regulatory mechanism on zinc finger E-box binding homobox 1(ZEB1).   Methods Real-time PCR technology was used to detect the expression of miR-128-3p in epithelial ovarian cancer tissue (EOC) and adjacent normal tissue(30 cases each), and to observe whether there was a difference. Two human ovarian cancer cell lines, SKOV3 and A2780, were selected and transfected respectively. MiR-128-3p mimic (miR-128-3p mimic group) and negative control mimic (NC mimic group) were used to detect the expression of miR-128-3p in 4 groups by Real-time PCR to verify the interference effect. Transwell assay was used. The migration and invasion abilities of the four groups of cells were observed. The regulatory relationship between miR-128-3p and ZEB1 was verified by dual luciferase assay, and the expression level of ZEB1 protein after overexpression of miR-128-3p was detected by Western blotting; pcDNA-ZEB1 was transfected into SKOV3 and A2780 cell lines to make it overexpression of ZEB1 was divided into NC mimic group, miR-128-3p mimic group, and miR-128-3p mimic+pcDNA-ZEB1 group. Western blot  ting was used to detect the EMT-related protein E-cadherin in 6 groups of cells and the expression level of vimentin.   Results Real-time PCR result showed that the expression of miR-128-3p in EOC tissues decreased compared with that in adjacent tissues(P<0.01); The relative expression of miR-128-3p in the miR-128-3p mimic group was higher than that in the NC mimic group, while the numbers of migrating cells and invasive cells were lower than those in the NC mimic group (P<0.01). The result  of dual luciferase experiments showed that miR-128-3p had a negative regulatory effect on ZEB1. In SKOV3 and A2780 cells, the relative expression of ZEB1 protein in the miR-128-3p mimic group was lower than that in the NC mimic group, while the relative protein expression of Ecadhein was higher than that in the NC mimic group (P<0.01). The relative protein expression of E-cadhein in the miR-128-3p mimic+pcDNA-ZEB1 group was lower than that in the miR-128-3p mimic group (P<0.01). In SKOV3 and A2780 cells, the relative protein expression of vimentin in the miR-128-3p mimic group was lower than that in the NC mimic group, and the relative p  rotein expression of vimentin in the miR-128-3p mimic+pcDNA-ZEB1 group was higher than that in the miR-128-3p mimic group (P<0.01).   Conclusion The expression of miR-128-3p decreases in epithelial ovarian cancer tissues, which may be due to the regulation of ZEB1 to affect the expression of EMT-related proteins and participate in the EMT process of ovarian cancer cells.