β淀粉样前体蛋白裂解酶1在大鼠颈上神经节组织中的表达

范娅楠; 李超红; 贾祥磊; 金家豪; 刘玉珍

doi:10.16098/j.issn.0529-1356.2022.06.002

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解剖学报 ›› 2022, Vol. 53 ›› Issue (6) : 698-704. DOI: 10.16098/j.issn.0529-1356.2022.06.002

神经生物学

β淀粉样前体蛋白裂解酶1在大鼠颈上神经节组织中的表达

范娅楠^1,2 李超红¹ 贾祥磊¹ 金家豪¹刘玉珍^1*

作者信息 +

Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues

FAN Ya-nan^1,2 LI Chao-hong¹ JIA Xiang-lei¹ JIN Jia-hao¹ LIU Yu-zhen^1*

Author information +

文章历史 +

摘要

目的观察β淀粉样前体蛋白裂解酶1（BACE1）在大鼠颈上神经节的表达和定位，及慢性间歇性低氧（CIH）对BACE1表达水平的影响。方法采用RT-PCR、Western blotting和免疫组织化学法检测颈上神经节BACE1的表达及分布。16只雄性SD大鼠随机分为对照（control）组和CIH组，每组8只，模型制作2周，RT-PCR检测CIH对BACE1、过氧化物酶体增殖物激活受体γ辅激活因子1α（PGC-1α）mRNA水平的影响。结果 BACE1表达于大鼠颈上神经节，主要分布于卫星胶质细胞和神经纤维，而在血管、神经元和小强荧光（SIF）细胞不表达。CIH下调BACE1 mRNA水平，上调PGC-1α mRNA水平（P<0.01）。结论 BACE1定位于大鼠颈上神经节卫星胶质细胞和神经纤维；BACE1水平降低可能参与调节CIH诱导的颈上神经节突触的可塑性。

Abstract

Objective To observe the expression and localization of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) in rat superior cervical ganglion and the effect of chronic intermittent hypoxia (CIH) on BACE1 level. Methods The expression and distribution of BACE1 in superior cervical ganglion were detected by RT-PCR, Western blotting and immunohistochemistry. Totally 16 male SD rats were randomly divided into control group and CIH group, 8 rats in each group. After 2 weeks of modeling, the effect of CIH on BACE1 and peroxisome proliferators activated receptor gamma co-activator 1 alpha (PGC-1α) mRNA level was detected by RT-PCR.

Results BACE1 was expressed in rat superior cervical ganglion, and mainly distributed in satellite glial cells and nerve fibers, but not in blood vessels, neurons and small intesely fluorecent(SIF) cells. CIH down-regulated BACE1 mRNA level, but up-regulated PGC-1α mRNA level (P<0.01). Conclusion BACE1 is located in satellite glial cells and nerve fibers in the superior cervical ganglion of rats. The decreased level of BACE1 may be involved in the regulation of CIH-induced synaptic plasticity of superior cervical ganglion.

导出引用

范娅楠李超红贾祥磊金家豪刘玉珍. β淀粉样前体蛋白裂解酶1在大鼠颈上神经节组织中的表达[J]. 解剖学报. 2022, 53(6): 698-704 https://doi.org/10.16098/j.issn.0529-1356.2022.06.002

FAN Ya-nan LI Chao-hong JIA Xiang-lei JIN Jia-hao LIU Yu-zhen. Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues[J]. Acta Anatomica Sinica. 2022, 53(6): 698-704 https://doi.org/10.16098/j.issn.0529-1356.2022.06.002

中图分类号： R34

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