心力衰竭大鼠心肌中弹性蛋白和胶原蛋白的表达

王柯柯; 罗欢欢; 孟祥光; 王前; 郭志坤

doi:10.16098/j.issn.0529-1356.2022.05.015

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解剖学报 ›› 2022, Vol. 53 ›› Issue (5) : 637-643. DOI: 10.16098/j.issn.0529-1356.2022.05.015

心力衰竭大鼠心肌中弹性蛋白和胶原蛋白的表达

王柯柯¹ 罗欢欢² 孟祥光¹ 王前¹ 郭志坤^1,3*

作者信息 +

Expression of elastin and collagen in rats with heart failure

WANG Ke-ke¹ LUO Huan-huan² MENG Xiang-guang¹ WANG Qian¹ GUO Zhi-kun^1,3*

Author information +

文章历史 +

摘要

目的探讨慢性心力衰竭大鼠心肌弹性蛋白和胶原蛋白及其相关蛋白酶的表达变化。方法 10只Sprague-Dawley大鼠腹腔注射盐酸阿霉素制作慢性心力衰竭模型，另10只腹腔注射等量生理盐水用作对照组。用超声心动图检查心脏功能，ELISA和免疫组织化学检测心脏脑钠肽（BNP），用Masson染色检测心肌纤维化。用Real-time PCR检测弹性蛋白（elastin）、Ⅰ/Ⅲ型胶原蛋白的mRNA变化，免疫荧光法和免疫印迹法检测elastin、Ⅰ/Ⅲ型胶原蛋白、基质金属蛋白酶2（MMP-2）和基质金属蛋白酶9（MMP-9）的蛋白表达变化。结果与对照组相比，模型组大鼠心功能下降，血清中BNP含量增加，心肌组织中BNP表达强阳性；elastin mRNA和蛋白表达水平下降，Ⅰ/Ⅲ型胶原蛋白mRNA和蛋白表达水平增加；MMP-2和MMP-9蛋白表达水平均增加。结论心力衰竭导致心肌组织弹性蛋白表达减少，Ⅰ/Ⅲ型胶原蛋白表达增加，MMP-2和MMP-9可能参与了这一变化过程。

Abstract

Objective To investigate the expression of elastin and collagen in heart of rats with chronic heart failure and their related proteases. Methods Sprague-Dawley rat model of chronic heart failure was made by intraperitoneal injection of doxorubicin hydrochloride. Examination of cardiac function by echocardiography. ELISA and immunohistochemistry were used to detect brain natriuretic peptide (BNP), and Masson staining was used to detect myocardial fibrosis. The mRNA levels of elastin, Ⅰ/Ⅲ collagen were detected by Real-time PCR and the expression levels of elastin, Ⅰ/Ⅲ collagen, Matrix metalloproteinase (MMP)-2 and MMP-9 were detected by Western blotting. Results Compared with the control group, the heart function of rats in the model group decreased, the BNP content in serum increased, the expression of BNP in myocardial tissue was strongly positive, and the mRNA and protein expression of elastin decreased. Nevertheless, the mRNA and protein expression of Ⅰ/Ⅲ collagen increased. At the same time, both MMP-2 and MMP-9 protein expression levels increased in model rat heart. Conclusion Heart failure leads to decreased expression of elastin, Ⅰ/Ⅲ collagen expression increased, and the MMP-2 and MMP-9 may be involved in the regulation of this process.

导出引用

王柯柯罗欢欢孟祥光王前郭志坤. 心力衰竭大鼠心肌中弹性蛋白和胶原蛋白的表达[J]. 解剖学报. 2022, 53(5): 637-643 https://doi.org/10.16098/j.issn.0529-1356.2022.05.015

WANG Ke-ke LUO Huan-huan MENG Xiang-guang WANG Qian GUO Zhi-kun. Expression of elastin and collagen in rats with heart failure[J]. Acta Anatomica Sinica. 2022, 53(5): 637-643 https://doi.org/10.16098/j.issn.0529-1356.2022.05.015

中图分类号： R331.5

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