微小RNA-29a-3p靶向Serpinh1调控人胃癌细胞系BGC823增殖和侵袭的分子机制

严晓娣; 刘贤称; 薛强; 王斐然; 顾红梅; 唐翀; 刘睿祺; 周友浪

doi:10.16098/j.issn.0529-1356.2022.05.010

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解剖学报 ›› 2022, Vol. 53 ›› Issue (5) : 607-612. DOI: 10.16098/j.issn.0529-1356.2022.05.010

微小RNA-29a-3p靶向Serpinh1调控人胃癌细胞系BGC823增殖和侵袭的分子机制

严晓娣¹ 薛强¹ 王斐然¹ 顾红梅¹ 唐翀² 刘睿祺³ 周友浪¹ 刘贤称^1*

作者信息 +

Molecular mechanism of microRNA-29a-3p targeting Serpinh1 regulating proliferation and invasion of human gastric cancer cell line BGC823

YAN Xiao-di¹ XUE Qiang¹ WANG Fei-ran¹ GU Hong-mei¹ TANG Chong² LIU Rui-qi³ ZHOU You-lang¹ LIU Xian-cheng^1*

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文章历史 +

摘要

目的探讨微小RNA（miR）-29a-3p影响Serpinh1对胃癌细胞的增殖与侵袭,并分析其相关的分子机制。方法检测miR-29a-3p在胃癌BGC823细胞中的表达水平，并评估miR-29a-3p在胃癌细胞增殖、迁移和侵袭中的作用。通过Western blotting和荧光素酶分析证明，miR-29a-3p与Serpinh1 3’UTR直接结合。此外，采用MTT法、集落形成法和Transwell法在体外检测miR-29a-3p/Serpinh1轴对胃癌细胞增殖、迁移和侵袭的影响。结果转染后miR-29a-3p模拟物（mimic）组中miR-29a-3p的表达明显高于miR-29a-3p阴性对照和空白组，转染miR-29a-3p mimic后BGC823细胞增殖能力显著降低。荧光素酶分析显示，miR-29a-3p通过靶向Serpinh1 3’-非翻译区（3’UTR）抑制Serpinh1的表达。此外，miR-29a-3p的过表达通过靶向Serpinh1显著抑制胃癌细胞的增殖、侵袭和迁移。

结论 MiR-29a-3p可靶向作用于Serpinh1，从而调控胃癌细胞的增殖与侵袭。

Abstract

Objective To investigate the effects of microRNA(miR)-29a-3p on the proliferation and invasion of gastric cancer cells and analyze its related molecular mechanism. Methods The expression level of miR-29a-3p in gastric cancer cells was detected, and the role of miR-29a-3p in the proliferation, migration, and invasion of gastric cancer cells was evaluated. Western blotting and luciferase analysis showed that miR-29a-3p was directly bound to Serpinh1 3’-untranslated region(3’UTR). In addition, the effects of the miR-29a-3p/Serpinh1 axis on the proliferation, migration, and invasion of gastric cancer cells were detected by MTT assay, colony formation assay, and Transwell assay in vitro. Results After transfection, the expression of miR-29a-3p in the miR-29a-3p mimic group was significantly higher than that in the miR-29a-3p negative control and blank group. After transfection, the proliferation of BGC823 cells decreased significantly. Luciferase analysis showed that miR-29a-3p inhibited the expression of Serpinh1 by targeting the 3’UTR of Serpinh1. In addition, overexpression of miR-29a-3p significantly inhibited the proliferation, invasion, and migration of gastric cancer cells by targeting Serpinh1. Conclusion MiR-29a-3p can target Serpinh1 and regulate the proliferation and invasion of gastric cancer cells.

导出引用

严晓娣薛强王斐然顾红梅唐翀刘睿祺周友浪刘贤称. 微小RNA-29a-3p靶向Serpinh1调控人胃癌细胞系BGC823增殖和侵袭的分子机制[J]. 解剖学报. 2022, 53(5): 607-612 https://doi.org/10.16098/j.issn.0529-1356.2022.05.010

YAN Xiao-di XUE Qiang WANG Fei-ran GU Hong-mei TANG Chong LIU Rui-qi ZHOU You-lang LIU Xian-cheng. Molecular mechanism of microRNA-29a-3p targeting Serpinh1 regulating proliferation and invasion of human gastric cancer cell line BGC823[J]. Acta Anatomica Sinica. 2022, 53(5): 607-612 https://doi.org/10.16098/j.issn.0529-1356.2022.05.010

中图分类号： R73.37

参考文献

［1］Sexton RE, Al Hallak MN, Diab M, et al. Gastric cancer: a comprehensive review of current and future treatment strategies［J］. Cancer Metastasis Rev, 2020, 39(4):1179-1203.

［2］Ford AC, Yuan Y, Moayyedi P. Helicobacter pylori eradication therapy to prevent gastric cancer: systematic review and meta-analysis［J］. Gut, 2020, 69(12):2113-2121.

［3］Hussen BM, Hidayat HJ, Salihi A, et al. MicroRNA: a signature for cancer progression［J］. Biomed Pharmacother, 2021, 138:111528.

［4］De Paolis V, Lorefice E, Orecchini E, et al. Epitranscriptomics: a new layer of microRNA regulation in cancer［J］. Cancers (Basel), 2021, 13(13):3372.

［5］Goodall GJ, Wickramasinghe VO. RNA in cancer［J］. Nat Rev Cancer, 2021, 21(1):22-36.

［6] Wang Y, Zhang X, Li H, et al. The role of miRNA-29 family in cancer［J］. Eur J Cell Biol, 2013, 92(3):123-128.

［7］Ghafouri-Fard S, Vafaee R, Shoorei H, et al. MicroRNAs in gastric cancer: biomarkers and therapeutic targets［J］. Gene, 2020, 757:144937.

［8］Kipkeeva F, Muzaffarova T, Korotaeva A, et al. MicroRNA in gastric cancer development: mechanisms and biomarkers［J］. Diagnostics (Basel), 2020, 10(11):891.

［9］ Liu DT, Yang ZhJ, Ding B, et al. Effects of microRNA-4286 on the growth，migration and invasion ofgastric cancer cell line HGC-27 by regulating inositol polyphosphate-4-phosphatase type I［J］. Acta Anatomica Sinica, 2021, 52(3):405-409. (in Chinese)

刘东涛，杨志娟，丁波，等. 微小RNA-4286调控肌醇多聚磷酸-4-磷酸酶Ⅰ型对胃癌细胞系HGC -27生长、迁移及侵的影响［J］. 解剖学报，2021,52（3）：405-409.

［10］Chen Y, Zhang W, Yan L, et al. MiR-29a-3p directly targets Smad nuclear interacting protein 1 and inhibits the migration and proliferation of cervical cancer HeLa cells［J］. Peer J, 2020, 8:e10148.

［11］Geng A, Luo L, Ren F, et al. MiR-29a-3p inhibits endometrial cancer cell proliferation, migration and invasion by targeting VEGFA/CD C42/PAK1［J］. BMC Cancer, 2021, 21(1):843.

［12］Han S, Wang Z, Liu J, et al. MiR-29a-3p-dependent COL3A1 and COL5A1 expression reduction assists sulforaphane to inhibit gastric cancer progression［J］. Biochem Pharmacol, 2021, 188:114539.

［13］Duarte BDP, Bonatto D. The heat shock protein 47 as a potential biomarker and a therapeutic agent in cancer research［J］. J Cancer Res Clin Oncol, 2018, 144(12):2319-2328.

［14］Wang F, Xue Q, Xu D, et al. Identifying the hub gene in gastric cancer by bioinformatics analysis and in vitro experiments［J］. Cell Cycle, 2020, 19(11):1326-1337.

［15］Tian S, Peng P, Li J, et al. SERPINH1 regulates EMT and gastric cancer metastasis via the Wnt/β-catenin signaling pathway［J］. Aging(Albany NY), 2020, 12(4):3574-3593.