C57BL/6小鼠胫骨前肌和趾长伸肌肌梭解剖学分析

连文玺; 段红梅; 郝飞; 郝鹏; 赵文; 高钰丹; 杨朝阳; 李晓光

doi:10.16098/j.issn.0529-1356.2022.02.010

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解剖学报 ›› 2022, Vol. 53 ›› Issue (2) : 203-209. DOI: 10.16098/j.issn.0529-1356.2022.02.010

C57BL/6小鼠胫骨前肌和趾长伸肌肌梭解剖学分析

连文玺¹ 段红梅² 郝飞¹ 郝鹏² 赵文² 高钰丹² 杨朝阳² 李晓光^1,2*

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Anatomical analysis of muscle spindles in tibialis anterior and extensor digitorum longus muscle of C57BL/6 mice

LIAN Wen-xi¹ DUAN Hong-mei² HAO Fei¹ HAO Peng² ZHAO Wen² GAO Yu-dan² YANG Zhao-yang² LI Xiao-guang^1,2*

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摘要

目的定位C57BL/6小鼠胫骨前肌（TA）和趾长伸肌（EDL）肌梭分布，分析肌梭在骨骼肌中的固定方式，并统计肌梭各区域长度和赤道横截面积（CAS）等参数分析肌梭形态学共性，为肌梭的形态和功能研究提供解剖学基础。方法 5只正常成年C57BL/6小鼠取TA和EDL，利用改良的骨骼肌冷冻技术得到无冰晶样本，样本连续冷冻切片，HE染色，显微成像，分析TA与EDL肌梭分布及肌梭在骨骼肌中与其他组织的连接方式。测量肌梭各区域长度和CAS，统计学分析肌梭形态特征。结果 C57BL/6小鼠TA和EDL分布情况为：从尾端至头端方向，肌梭主要分布在肌腹中间偏上位置。从背侧至腹侧，肌梭靠近腓深神经入肌点分布。在肌梭末梢可以看到肌梭锚定连接梭外纤维并固定于骨骼肌中。单个肌梭形态观察分析显示，连接感觉神经纤维末梢的区域 A和与运动神经纤维末梢连接区域B的长度有较明显相关性（相关系数为0.75）。结论 C57BL/6小鼠TA和EDL肌梭分布特点可以为后续肌梭相关的形态学和电生理研究提供解剖信息，发现肌梭区域 A和区域B 的相关性可能有助于解释肌梭信号传递能力差异。

Abstract

Objective To locate the distribution of muscle spindles in tibialis anterior (TA) and extensor digitorum longus muscle (EDL) and the anchoring mode of muscle spindles in skeletal muscles, and perform statistics analysis of their morphological character by anatomical parameters. Methods Five adult wild type C57BL/6 mice were sacrificed, and TA and EDL were dissected and frozen with improved ultra-low temperature cryopreservation technology avoiding myofibers damaged by possible ice crystal. Continuous frozen transections were obtained and operated by HE staining, followed by microimaging to spot the muscle spindles location. Some parameters including regions length and cross section area (CSA) of muscle spindles were noticed for the discovery of some general characteristics of spindles by statistics. Results For TA and EDL, the scattered characters of muscle spindles were distributed as follows: the spindles were located at the upper third of the mid-belly of both TA and EDL from caudal to rostral position, while near the enter point to muscle of the deep peroneal nerve in dorsal-ventral orientation. The peripheral of muscle spindles anchored to extrafusal fibers to hold in the muscle. And in term of length, region A, connected with sensory nerve ending, demonstrated a significant correlation with region B, which located at the poles of region A and twined by motor nerve ending (correlation index=0.75) when considering the muscle spindles with four intrafusal fibers only. And no correlation was discovered in any others pairwise parameters. Conclusion The scattered diagram of muscle spindles in TA and EDL of C57BL/6 mice might provide anatomic basis for evaluation of lower limb motor function, especially for the spinal cord injury and recovery research. And the correlationship between the length of region A and B might improve exploring the variability of electrophysiological characters.

导出引用

连文玺段红梅郝飞郝鹏赵文高钰丹杨朝阳李晓光. C57BL/6小鼠胫骨前肌和趾长伸肌肌梭解剖学分析[J]. 解剖学报. 2022, 53(2): 203-209 https://doi.org/10.16098/j.issn.0529-1356.2022.02.010

LIAN Wen-xi DUAN Hong-mei HAO Fei HAO Peng ZHAO Wen GAO Yu-dan YANG Zhao-yang LI Xiao-guang. Anatomical analysis of muscle spindles in tibialis anterior and extensor digitorum longus muscle of C57BL/6 mice[J]. Acta Anatomica Sinica. 2022, 53(2): 203-209 https://doi.org/10.16098/j.issn.0529-1356.2022.02.010

中图分类号： R332

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