目的  以人附睾组织为研究对象，进行附睾管三维重建，明确附睾管解剖学及组织学特点。   方法  对1例人附睾连续组织切片，LeicaAperio AT2数字扫描，Photoshop CC 2018配准，VGStudio MAX V3.0三维立体合成，Materialise Magics V22.0后期修饰。另1例用于电子显微镜观察。结合切片及三维重建，分析附睾管的组织学特点。   结果  获得7 μm厚的横断面人附睾石蜡切片4331张，矢状面切片543张；人附睾管存在明显的区域性分布，附睾头、体和尾部可分别划分为7个、9个和4个亚区，亚区间有组织间隔；不同亚区内附睾管排列无序，但附睾管管径及其上皮结构存在差异，且体、尾部各亚区间为单根附睾管连接。   结论  利用连续组织切片的方法能成功三维重建人附睾管。揭示人附睾管具有明显的空间区域性，不同亚区组织存在差异。

Objective  Based on the techniques of continual tissue slices, the human epididymis was rebuilt for understanding the anatomical and histological features of the epididymal ducts.    Methods  Continuous tissue slices of one human epididymis were performed and digital slice images were obtained through scanning with Leica-Aperio AT2; the pipe wall of epididymal duct was aligned in sequence with Photoshop CC 2018 software and VGStudio MAX V3.0 software was used for three-dimensional synthesis. Finally, the later modification was carried out with Materialise Magics V22.0 software. Another human epididymis was used for electron microscopy. The histological features of epididymal ducts were analysed by combining slices and three-dimensional reconstruction.    Results  A total of 4331 and 543 slices in transverse and sagittal section respectively were prepared with a thickness of 7 μm. According to the three-dimensional structure, regional distribution of human epididymal duct could be found obviously and the caput, corpus and cauda of epididymis could be clearly divided into 7, 9 and 4 subregions respectively. There were tissue intervals among adjacent subregions and epididymal ducts were disordered within each subregion, but differences were existed in tubular diameter and epithelial structure, and adjacent subregions were connected by single epididymal duct in the corpus and cauda.    Conclusion  The human epididymal duct can be successfully reconstructed by continual tissue slices technique. The human epididymal duct has regional distribution in space obviously and there are differences between different subregions.