大鼠肝再生启动阶段肝细胞CCAAT 增强子结合蛋白δ mRNA、微小RNA-3553和rno-Acad8_0002的表达和作用

徐存拴

doi:10.16098/j.issn.0529-1356.2021.06.012

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解剖学报 ›› 2021, Vol. 52 ›› Issue (6) : 917-920. DOI: 10.16098/j.issn.0529-1356.2021.06.012

肝再生与细胞周期调控

大鼠肝再生启动阶段肝细胞CCAAT 增强子结合蛋白δ mRNA、微小RNA-3553和rno-Acad8_0002的表达和作用

李亚霏^1,2王子慧^1,2 臧夏炎^1,2 靳伟^1,2 常翠芳^1,2 郭建林^1,2* 徐存拴^1,2*

作者信息 +

Expression and role of CCAAT enhancer binding protein δ mRNA, microRNA-3553 and rno-Acad8_0002 of hepatocytes during the rat liver regeneration initiation

LI Ya-fei^{1, 2} WANG Zi-hui^{1, 2} ZANG Xia-yan^{1, 2} JIN Wei^{1, 2} CHANG Cui-fang^{1, 2} GUO Jian-lin^{1, 2*} XU Cun-shuan^{1, 2*}

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摘要

目的了解大鼠肝再生启动阶段CCAAT 增强子结合蛋白 δ（CEBPδ）mRNA、miR3553和rno-Acad8_0002调节肝细胞处于G0期还是G1期的途径和方法。方法按Higgins等方法制备大鼠2/3肝切除（PH）模型，按Smedsrod等方法分离肝细胞，用大规模定量分析技术检测大鼠肝再生中肝细胞竞争性内源RNA（ceRNA）表达的变化，用Cytoscape 3.2软件构建ceRNA的相互作用网络,用ceRNA综合分析等方法解析它们表达和作用的相关性。结果 PH后0 h和 6 h时，CEBPδ mRNA的比0.40±0.08和2.15±0.24，miR-3553为2.53±0.47和1.17±0.31，rno-Acad8_0002为1.24±0.04和2.66±0.54。CEBPδ抑制的G0期相关基因转化生长因子β受体2（TGFBR2）为2.77±0.20和0.79±0.13，磷脂酶A2-IVA（PLA2G4A）为2.56±0.76和0.42±0.13。CEBPδ促进的G1期相关基因纤溶酶原激活剂尿激酶受体（PLAUR）为0.27±0.08和2.62±0.31，丝裂原活化蛋白激酶14（MAPK14）为1.01±0.15和2.01±0.32，ETS变异转录因子6（ETV6）为0.77±0.05和2.22±0.68，血红蛋白加氧酶1（HMOX1）为1.05±0.21和4.57±0.88。结论 PH后0 h时，CEBPδ mRNA下调，有利于CEBPδ抑制的G0期相关基因表达和肝细胞处于G0期。相反，PH后6 h时，rno-Acad8_0002和miR-3553的相互作用解除了后者对CEBPδ mRNA的抑制，有利于CEBPδ形成，有利于CEBPδ促进的G1期相关基因表达和肝细胞处于G1期。

Abstract

Objective To explore the pathways and patterns which the CCAAT enhancer binding protein δ(CEBPδ) mRNA, miR-3553 and rno-Acad8_0002 regulate the hepatocytes in G0 phase and G1 phase during rat liver regeneration (LR). Methods The rat 2/3 partial hepatectomy (PH) model was prepared as described by Higgins, the hepatocytes of rat liver right lobes were isolated in 9∶00-11∶00 am according to the method of Smedsrod et al, a large-scale quantitative detection of competitive endogenous RNA(ceRNAs) was processed by the high-throughput biotechnology, the interaction network of ceRNAs was constructed by Cytoscape 3.2 software, and the correlation in expression and role of ceRNAs was analyzed by ceRNA comprehensive analysis. Results It was found that at 0 hour and 6 hours after PH, the ratio value of CEBPδ mRNA showed 0.40±0.08 and 2.15±0.24, miR-3553 displayed 2.53±0.47 and 1.17±0.31, rno-Acad8_0002 indicated 1.24±0.04 and 2.66±0.54. The G0 phase-related genes inbibited by CEBPδ were following, the transforming growth factor beta receptor 2 (TGFBR2) were 2.77±0.20 and 0.79±0.13, the phospholipase A2 group IVA (PLA2G4A) were 2.56±0.76 and 0.42±0.13. The G1 phase-related genes promoted by CEBPδ were following, the plasminogen activator urokinase receptor (PLAUR) were 0.27±0.08 and 2.62±0.31, the mitogen-activated protein kinase 14 (MAPK14) 1.01±0.15 and 2.01±0.32, the ETS variant transcription factor 6 (ETV6) were 0.77±0.05 and 2.22±0.68, the hemoglobin oxygenase 1 (HMOX1) were 1.05±0.21 and 4.57±0.88. Conclusion CEBPδ mRNA is down-regulated at 0 hour after PH, that is helpful for the expression of the G0 phase-related genes inhibited by CEBPδ and for the hepatocytes to be in G0 phase. On the contrary, the interaction of miR-3553 and rno-Acad8_0002 leads to CEBPδ mRNA to bind with miR-3553, to CEBPδ being formed probablely, and to the expression of the G1 phase-related genes promoted by CEBPβ probablely, and to the hepatocytes being in G1 phase at 6 hours after PH.

导出引用

李亚霏王子慧臧夏炎靳伟常翠芳郭建林徐存拴. 大鼠肝再生启动阶段肝细胞CCAAT 增强子结合蛋白δ mRNA、微小RNA-3553和rno-Acad8_0002的表达和作用[J]. 解剖学报. 2021, 52(6): 917-920 https://doi.org/10.16098/j.issn.0529-1356.2021.06.012

LI Ya-fei WANG Zi-hui ZANG Xia-yan JIN Wei CHANG Cui-fang GUO Jian-lin XU Cun-shuan. Expression and role of CCAAT enhancer binding protein δ mRNA, microRNA-3553 and rno-Acad8_0002 of hepatocytes during the rat liver regeneration initiation[J]. Acta Anatomica Sinica. 2021, 52(6): 917-920 https://doi.org/10.16098/j.issn.0529-1356.2021.06.012

中图分类号： Q257

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