大鼠成纤维细胞生长因子对心肌成纤维细胞增殖和转分化的作用机制

刘梦昕; 孙昌业; 任明芬; 郭志坤; 李铎

doi:10.16098/j.issn.0529-1356.2021.05.007

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解剖学报 ›› 2021, Vol. 52 ›› Issue (5) : 712-719. DOI: 10.16098/j.issn.0529-1356.2021.05.007

细胞和分子生物学

大鼠成纤维细胞生长因子对心肌成纤维细胞增殖和转分化的作用机制

刘梦昕¹ 孙昌业¹ 李铎¹任明芬²郭志坤^1*

作者信息 +

Effect and mechanism of fibroblast growth factor on proliferation and transdifferentiation of cardiac fibroblasts in rats#br#

LIU Meng-xin¹ SUN Chang-ye¹ Li Duo¹ REN Ming-fen² GUO ZhI-kun^1*#br#

Author information +

文章历史 +

摘要

目的探讨成纤维细胞生长因子（FGF）对心肌成纤维细胞(CFs)在增殖和转分化为肌成纤维细胞（MFs）中的作用。方法分离、培养大鼠CFs，利用FGF对其进行诱导培养，CCK-8技术检测细胞活性和增殖状况，免疫荧光和Western blotting技术测定α平滑肌肌动蛋白（α-SMA）和Ⅰ型胶原（ColⅠ）的表达量。结果随大鼠CFs培养代数的增加，其α-SMA、ColⅠ的表达和活化为MFs的数量增加。加入FGF诱导培养的大鼠CFs数量没有明显增加；加入FGF1、FGF2诱导的CFs表达α-SMA减少，活化为MFs 数量减少。结论 FGF家族对大鼠CFs没有促增殖作用，但FGF1和FGF2可以抑制CFs活化，减少其向MFs的分化。

Abstract

Objective To investigate the effect of fibroblast growth factor (FGF) on the proliferation and transdifferentiation of cardiac fibroblasts (CFs) into myofibroblasts (MFs). Methods Rat CFs were isolated and cultured, and then induced by FGF. CCK-8 was used to detect the cell activity and proliferation. Immunofluorescence and Western blotting were used to detect the expression of α smooth muscle actin (α-SMA) and collagen Ⅰ (ColⅠ). Results The expression and activation of α-SMA and ColⅠ increased with the increase of CFs culture generation. The number of CFs induced by FGF did not increased significantly; the expression of α-SMA in CFs induced by FGF1 and FGF2 decreased, and the number of activated MFs decreased. Conclusion FGF family has no effect on the proliferation of CFs, but FGF1 and FGF2 can inhibit the activation of CFs and reduce the differentiation into MFs.

导出引用

刘梦昕孙昌业李铎任明芬郭志坤. 大鼠成纤维细胞生长因子对心肌成纤维细胞增殖和转分化的作用机制[J]. 解剖学报. 2021, 52(5): 712-719 https://doi.org/10.16098/j.issn.0529-1356.2021.05.007

LIU Meng-xin SUN Chang-ye Li Duo REN Ming-fen GUO ZhI-kun. Effect and mechanism of fibroblast growth factor on proliferation and transdifferentiation of cardiac fibroblasts in rats#br#[J]. Acta Anatomica Sinica. 2021, 52(5): 712-719 https://doi.org/10.16098/j.issn.0529-1356.2021.05.007

中图分类号： R34

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