自主跑轮运动通过促进海马神经发生改善甲醛所致小鼠的负性情绪

李威; 周鑫; 谢志艳; 张天乐; 杨惠; 曹文宇; 刘志文; 曾佳玉

doi:10.16098/j.issn.0529-1356.2021.05.003

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解剖学报 ›› 2021, Vol. 52 ›› Issue (5) : 686-691. DOI: 10.16098/j.issn.0529-1356.2021.05.003

神经生物学

自主跑轮运动通过促进海马神经发生改善甲醛所致小鼠的负性情绪

李威¹ 周鑫¹ 谢志艳¹ 张天乐¹杨惠² 曹文宇¹ 刘志文^3*曾佳玉^1*

作者信息 +

Voluntary wheel running prevents the formaldehyde induced negativeemotion through up-regulation of hippocampal neurogenesis in mice#br#

LI Wei¹ ZHOU Xin¹ XIE Zhi-yan¹ ZHANG Tian-le¹ YANG Hui² CAO Wen-yu¹ LIU Zhi-wen^3* ZENG Jia-yu^1*

Author information +

文章历史 +

摘要

目的探讨自主跑轮运动对甲醛疼痛模型小鼠负性情绪的影响。方法 30只昆明雄鼠随机分成生理盐水对照组（NS）、甲醛组（F）、自主跑轮运动+甲醛组（R+F）。右后爪足底皮下注射0.4%甲醛建立疼痛模型，R+F组在模型建立之前置于小鼠跑轮运动装置3周。以实验小鼠舔足累计时间评价自发痛行为；采用旷场实验（OFT）和高架十字迷宫实验（EPM）检测小鼠焦虑样行为；采用被迫游泳实验（FST）检测小鼠抑郁样行为；采用免疫组织化学方法检测海马未成熟神经元标记物双皮层蛋白（DCX）的表达情况。结果自发痛评分结果显示，与NS组相比，F组小鼠出现典型的双相痛反应，与F组相比， R+F组小鼠的第Ⅱ相时段舔咬足累计时间明显缩短（P<0.01）。旷场实验结果显示，与NS组相比，F组小鼠在中央格探索时间显著减少（P<0.001），与F组相比，R+F组小鼠在中央格探索的时间增加（P<0.05）。高架十字迷宫实验结果显示，与NS组相比，F组小鼠进入开放臂的时间显著减少（P<0.001），与F组相比，R+F组小鼠进入开放臂的时间增加（P<0.05）。被迫游泳实验结果显示，与NS组相比，F组小鼠不动时间明显增加（P<0.01），与F组相比，R+F组小鼠不动时间缩短（P<0.05）。免疫组织化学结果显示，与NS组（250.90±16.22）相比，F组小鼠海马DCX阳性细胞面积（210.90±7.98）减少，与F组相比，R+F组小鼠海马DCX阳性细胞面积显著增多（284.00±9.84）。结论自主跑轮运动可能通过促进海马神经发生，从而改善甲醛所致小鼠的自发痛与焦虑、抑郁样行为。

Abstract

Objective To investigate the effect of voluntary wheel running on negative affective of mice induced by formaldehyde. Methods Thirty male Kunming mice were randomly divided into three groups, including normal saline control group (NS), formaldehyde model group (F), and voluntary wheel running with formaldehyde injection group (R+F). The pain model was established by right hindpaw intraplantar formalin injection, the mice of R+F group experienced voluntary wheel running for three weeks before intraplantar formaldehyde injection. The spontaneous pain behavior was determined by the cumulative time of licking paw. The anxiety-like behavior of each group was determined by open field test (OFT) and elevated plus-maze test (EPM) while the depression-like behavior of each group was determined by forced swimming test (FST). The expression of doublecortin（DCX）in the hippocampus was determined by immunohistochemistry. Results Compared with the NS group, the typical two-phase pain response was observed in the F group, and compared with the F group，the second phase pain duration was significantly reduced in the R+F group (P<0.01). In the open field test, the F group showed remarkably reduced time in the inner area(P<0.001) compared with the NS group, while the R+F group increased time in the inner area (P<0.05) compared with the F group. In the elevated plus-maze test, the F group showed remarkably reduced time (P<0.001)spent in the open arm compared with the NS group，however，compared with the F group, R+F group increased time spent in the open arm (P<0.05). In the forced swimming test, the immobility time of the F group significant increased (P<0.01) compared with the NS group, which was decreased in the R+F group (P<0.05). The Immunohistochemistry showed that the area of DCX positive cells in the hippocampus of the F group was downregulated compared with the NS group, which was upregulated in the R+F group. Conclusion Our findings indicate that voluntary wheel running can improve anxiety and depression-like in mice induced by formaldehyde injection, which may be related to enhanced neurogenesis in the hippocampus.

导出引用

李威周鑫谢志艳张天乐杨惠曹文宇刘志文曾佳玉. 自主跑轮运动通过促进海马神经发生改善甲醛所致小鼠的负性情绪[J]. 解剖学报. 2021, 52(5): 686-691 https://doi.org/10.16098/j.issn.0529-1356.2021.05.003

LI Wei ZHOU Xin XIE Zhi-yan ZHANG Tian-le YANG Hui CAO Wen-yu LIU Zhi-wen ZENG Jia-yu. Voluntary wheel running prevents the formaldehyde induced negativeemotion through up-regulation of hippocampal neurogenesis in mice#br#[J]. Acta Anatomica Sinica. 2021, 52(5): 686-691 https://doi.org/10.16098/j.issn.0529-1356.2021.05.003

中图分类号： R322.81

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