大鼠肝再生中环状RNA表达谱的变化及其对自噬的影响

王棋文; 姚奋杰; 杨卓菲; 郭学强; 穆萌萌; 周运; 徐存拴

doi:10.16098/j.issn.0529-1356.2021.04.013

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解剖学报 ›› 2021, Vol. 52 ›› Issue (4) : 581-588. DOI: 10.16098/j.issn.0529-1356.2021.04.013

细胞和分子生物学

大鼠肝再生中环状RNA表达谱的变化及其对自噬的影响

王棋文^1,2 姚奋杰^1,2 杨卓菲^1,2郭学强^1,2穆萌萌^1,2周运^1,2,3 徐存拴^1,2*

作者信息 +

Expression profile of circular RNA and its regulatory effect on autophagy during rat liver regeneration

WANG Qi-wen^1,2 YAO Fen-jie^1,2 YANG Zhuo-fei^1,2 GUO Xue-qiang^1,2 MU Meng-meng^1,2 ZHOU Yun^1,2,3 XU Cun-shuan^1,2*

Author information +

文章历史 +

摘要

目的探讨大鼠肝再生中环状RNA（circRNA）的表达变化以及对自噬的影响。方法以60只大鼠 2/3 肝切除0 h、 2 h、6 h、12 h、24 h、30 h、36 h、72 h、120 h和168 h后的再生肝circRNAs、微小RNAs（miRNAs）、mRNA的高通量测序数据为基础，分析其在肝再生中的表达变化，进一步用miRanda aug 2010软件预测circRNA-miRNA和miRNA-mRNA靶标关系，并根据基因功能→miRNA功能→circRNA功能反向推测得到支配大鼠肝脏自噬的肝再生相关circRNAs和miRNAs，并构建它们的相互作用网络。结果与肝再生自噬相关的circRNAs、miRNAs、mRNA分别有102、42、153种。利用生物信息学分析，发现44种circRNAs、13种miRNAs及17种mRNA之间有相互作用关系，并以此构建了它们的共表达网络。结论筛选出了与肝再生自噬相关的circRNAs、miRNAs和mRNA，并构建了相互作用网络图谱。

Abstract

Objective Studies have shown that circular RNA (circRNA) is a molecular sponge of microRNA(miRNA) and can be used for the treatment and diagnosis of liver diseases. Therefore, this study aims to further investigate the expression profile of circRNA and its effect on autophagy in rat liver regeneration. Methods Based on the high-throughput sequencing in regenerated liver of 0 hour, 2, 6, 12, 24, 30, 36, 72, 120 and 168 hours after 2/3 hepatectomy in 60 rats, the expression profiles of circRNAs, miRNAs and mRNA were analyzed and their relationship were predicted by miRanda aug 2010 software. Furthermore, according to gene function → miRNA function → circRNA function, circRNAs and miRNAs that regulating autophagy were obtained in liver regeneration and compting endogenous(ceRNA) network was constructed. Results There were 102 circRNAs, 42 miRNAs and 153 mRNAs associated with autophagy involved in liver regeneration. Using bioinformatics analysis, 44 types of circRNAs, 13 types of miRNAs and 17 types of mRNA were found to interact with each other, and their co-expression network was constructed. Conclusion In this study, we screened out the circRNAs, miRNAs and mRNAs related to autophagy in liver regeneration and built the interaction network map.

导出引用

王棋文姚奋杰杨卓菲郭学强穆萌萌周运徐存拴. 大鼠肝再生中环状RNA表达谱的变化及其对自噬的影响[J]. 解剖学报. 2021, 52(4): 581-588 https://doi.org/10.16098/j.issn.0529-1356.2021.04.013

WANG Qi-wen YAO Fen-jie YANG Zhuo-fei GUO Xue-qiang MU Meng-meng ZHOU Yun XU Cun-shuan. Expression profile of circular RNA and its regulatory effect on autophagy during rat liver regeneration[J]. Acta Anatomica Sinica. 2021, 52(4): 581-588 https://doi.org/10.16098/j.issn.0529-1356.2021.04.013

中图分类号： Q954

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