目的  探讨性别决定区Y框蛋白4（SOX4）在子宫内膜癌组织中的表达水平及其生物学作用。   方法  收集156例子宫内膜癌组织和子宫内膜癌癌旁组织，另取156例子宫内膜不典型增生组织，采用免疫组织化学法检测子宫内膜癌、子宫内膜不典型增生和癌旁组织中SOX4的表达，并分析SOX4表达与子宫内膜癌患者临床特征的关系。采用慢病毒转染的技术方法建立SOX4过表达/沉默Ishikawa细胞株后，MTT、流式细胞术和Transwell小室法检测细胞的增殖、凋亡、迁移和侵袭能力，Western blotting检测SOX4、β-连环蛋白(β-catenin)和上皮型钙黏附蛋白(E-cadherin)的表达变化。   结果  SOX4在子宫内膜癌组织中的表达高于癌旁组织和子宫内膜不典型增生组织(P<0.05)。SOX4表达与侵袭深度、国际妇产科联合会（FIGO）分期和淋巴结转移相关(P<0.05)。与对照组相比，SOX4过表达的Ishikawa细胞的增殖能力、迁移和侵袭能力显著增加，细胞凋亡率明显降低，蛋白SOX4和β-catenin表达水平明显升高，蛋白E-cadherin表达水平明显降低(均P<0.05)。SOX4干扰的Ishikawa细胞的增殖能力、迁移和侵袭能力显著降低，细胞凋亡率明显升高，蛋白SOX4和β-catenin表达水平明显降低，蛋白E-cadherin表达水平明显升高(均P<0.05)。   结论  SOX4在子宫内膜癌组织中高表达，可能通过激活Wnt/β-catenin信号通路促进子宫内膜癌的发生发展。

Objective  To investigate the expression of sex determining region Y box protein 4(SOX4) gene and its biological effects in endometrial carcinoma.    Methods  156 cases of endometrial carcinoma tissues, adjacent tissues of endometrial carcinoma and 156 cases of endometrial atypical hyperplasia were collected; Immunohistochemistry was used to detect the expression of SOX4 in endometrial cancer, endometrial dysplasia and normal endometrial tissue, and to analyze the relationship between SOX4 and the clinical characteristics of patients with endometrial cancer. After establishing the SOX4 overexpression/silencing Ishikawa cell strain using the lentivirus transfection technique, MTT, flow cytometry and Transwell chamber method  were used to detect the cell proliferation, apoptosis, migration and invasion ability, and Western blotting method  was used to detect SOX4, βcatenin and E-cadherin protein expression changes.     Results  The expression of SOX4 in endometrial cancer tissue was higher than that in normal endometrial tissue and endometrial atypical hyperplasia (P<0.05). SOX4 expression was correlated with invasion depth, International Federation of Obstetrics and Gynecoogy(FIGO) stage and lymph node metastasis (P<0.05). Compared with control group, SOX4 overexpressed Ishikawa cells have significantly increased proliferative ability, migration and invasion ability, significantly reduced apoptosis rate, significantly increased SOX4 and β-catenin protein expression, and significantly reduced E-cadherin protein expression (all P<0.05). while the proliferation ability, migration and invasion ability of Ishikawa cells interfered by SOX4 were significantly reduced, the apoptosis rate was significantly increased, the expression of SOX4 and β-catenin protein was significantly reduced, and the expression of E-cadherin protein was significantly increased (all P<0.05).     Conclusion  SOX4 gene is highly expressed in endometrial cancer tissues, which may promote the development of endometrial cancer by activating Wnt/β-catenin signaling pathway.