微小RNA-4286调控肌醇多聚磷酸-4-磷酸酶Ⅰ型对胃癌细胞系HGC-27生长、迁移及侵袭的影响

刘东涛

doi:10.16098/j.issn.0529-1356.2021.03.012

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解剖学报 ›› 2021, Vol. 52 ›› Issue (3) : 405-409. DOI: 10.16098/j.issn.0529-1356.2021.03.012

细胞和分子生物学

微小RNA-4286调控肌醇多聚磷酸-4-磷酸酶Ⅰ型对胃癌细胞系HGC-27生长、迁移及侵袭的影响

刘东涛杨志娟丁波马俊文*

作者信息 +

Effects of microRNA-4286 on the growth, migration and invasion of gastric cancer cell line HGC-27 by regulating inositol polyphosphate-4-phosphatase type Ⅰ

LIU Dong-tao YANG Zhi-juan DING Bo MA Jun-wen*

Author information +

文章历史 +

摘要

目的探讨微小RNA-4286（miRNA-4286）调控肌醇多聚磷酸-4-磷酸酶Ⅰ型（INPP4A）对胃癌细胞系NGC-27生长、迁移及侵袭的影响。方法将胃癌HGC-27细胞分为空白对照组和阴性对照组、干预组，空白对照组不作任何处理，阴性对照组转染pEGFP-N1质粒，干预组转染pEGFP-N1-miRNA-4286质粒，采用Real-time PCR检测3组胃癌HGC-27细胞miRNA-4286表达量，采用Western blotting检测3组胃癌HGC-27细胞中INPP4A蛋白表达量，采用八肽胆囊收缩素（CCK8）法检测3组胃癌HGC-27细胞增殖率，采用Transwell小室侵袭实验检测3组胃癌HGC-27细胞侵袭能力，采用划痕实验检测3组胃癌HGC-27细胞迁移能力。结果干预组miRNA-4286表达量、增殖率、穿过小室膜的HGC-27细胞数明显高于空白对照组和阴性对照组（P＜0.05）；干预组INPP4A蛋白表达量、划痕伤口愈合间距明显低于空白对照组和阴性对照组（P＜0.05）；阴性对照组miRNA-4286表达量、INPP4A蛋白表达量、增殖率、穿过小室膜的HGC-27细胞数、划痕伤口愈合间距较空白对照组差异无统计学意义（P＞0.05）。结论 MiRNA-4286可能通过下调INPP4A表达而促进胃癌细胞系HGC-27生长、迁移及侵袭。

Abstract

Objective To investigate the effects of microRNA-4286 (miRNA-4286) on the growth, migration and invasion of gastric cancer cell line HGC-27 by regulating inositol polyphosphate-4-phosphatase type Ⅰ (INPP4A). Methods HGC-27 cells were divided into blank control group, negative control group and intervention group. The blank control group received no treatment, negative control group was transfected with pEGFP-N1 plasmid, and intervention group was transfected with pEGFP-N1-miRNA-4286 plasmid. Real-time PCR was used to detect the expression of miRNA-4286 in HGC-27 cells, and Western blotting was used to detect the expression of INPP4A protein in HGC-27 cells. The proliferation rate, invasion ability and migration ability of HGC-27 cells were detected by cholecystokinin octapeptide, cholecystokinin octapeptide(CCK8), Transwell chamber assay and scratch test, respectively. Results The miRNA-4286 expression level, proliferation rate, and number of transmembrane cells in intervention group were significantly higher than those in blank control group and negative control group (P<0.05). The expression level of INPP4A protein and the scratch healing ability of intervention group were significantly lower than those of blank control group and negative control group (P<0.05). The miRNA-4286 expression, INPP4A protein expression, proliferation rate, number of transmembrane cells, and the scratch healing ability of negative control group had no significant difference with those of blank control group (P>0.05). Conclusion miRNA-4286 may promote the growth, migration and invasion of gastric cancer cell line HGC-27 by down-regulating the expression of INPP4A.

导出引用

刘东涛杨志娟丁波马俊文. 微小RNA-4286调控肌醇多聚磷酸-4-磷酸酶Ⅰ型对胃癌细胞系HGC-27生长、迁移及侵袭的影响[J]. 解剖学报. 2021, 52(3): 405-409 https://doi.org/10.16098/j.issn.0529-1356.2021.03.012

LIU Dong-tao YANG Zhi-juan DING Bo MA Jun-wen. Effects of microRNA-4286 on the growth, migration and invasion of gastric cancer cell line HGC-27 by regulating inositol polyphosphate-4-phosphatase type Ⅰ[J]. Acta Anatomica Sinica. 2021, 52(3): 405-409 https://doi.org/10.16098/j.issn.0529-1356.2021.03.012

中图分类号： R735.2

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