上调锌指和含BTB 域20表达对APPswe/PSE9转基因阿尔茨海默病小鼠学习记忆能力的影响

王月静; 孙丽慧

doi:10.16098/j.issn.0529-1356.2021.03.006

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解剖学报 ›› 2021, Vol. 52 ›› Issue (3) : 365-369. DOI: 10.16098/j.issn.0529-1356.2021.03.006

神经生物学

上调锌指和含BTB 域20表达对APPswe/PSE9转基因阿尔茨海默病小鼠学习记忆能力的影响

王月静¹张萌¹ 郎尉雅¹ 张海燕¹ 廉洁¹冯化杰² 孙丽慧^1*

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Effects of up-regulation of zinc finger and BTB domain containing 20 expression on learning and memory of APPswe/PSE9 transgenic Alzheimer disease mice

WANG Yue-jing¹ ZHANG Meng¹ LANG Wei-ya¹ ZHANG Hai-yan¹LIAN Jie¹ FENG Hua-jie² SUN Li-hui^1*

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文章历史 +

摘要

目的探讨上调锌指和含BTB域20（A20）表达对APPswe/PSE9转基因阿尔茨海默病（AD）小鼠学习记忆影响及其对海马神经元凋亡的抑制作用。方法将APPswe/PSE9转基因小鼠40只分为4组，分别为对照组、AD组、干扰小RNA（siRNA）-AD组和A20-AD组。选择携带高表达A20的腺病毒为干扰药物，采用Morris水迷宫实验观察小鼠空间学习记忆能力，采用Westren blottong技术检测A20的表达，采用免疫组织化学法观察各种小鼠海马CA1区P53表达情况。结果与AD组比较，A20-AD组小鼠学习记忆功能有所改善，海马CA1区神经元凋亡率显著降低，海马CA1区P53表达显著降低。结论上调A20表达能提升APPswe/PSE9转基因小鼠学习记忆能力，可使APPswe/PSE9转基因小鼠海马CA1区P53表达降低。

Abstract

Objective To investigate the effects of up-regulation of zinc finger and BTB domain containing 20（A20）expression on learning and memory and apoptosis of hippocampal neurons in APPswe/PSE9 transgenic Alzheimer disease（AD）mice. Methods Forty APPswe/PSE9 transgenic mice were divided into four groups: control group, AD group, small interfering RNA(siRNA）-AD group and A20-AD group. Adenoviruses carrying high expression of A20 were selected as interfering drugs and Morris water maze was used to observe spatial learning and memory. Western blotting was used to detect the expression of A20. Immunohistochemistry was used to observe the expression of P53 in the CA1 region. Results Compared with the AD group, the learning and memory function was mproved, the apoptosis rate of hippocampal CA1 neurons decreased significantly and the expression of P53 in the CA1 region decreased significantly in A20-AD group mice. Conclusion Upregulation of A20 expression can improve the learning and memory ability of transgenic mice, reduce the expression of P53 in hippocampal CA1 region of the transgenic mice.

导出引用

王月静张萌郎尉雅张海燕廉洁冯化杰孙丽慧. 上调锌指和含BTB 域20表达对APPswe/PSE9转基因阿尔茨海默病小鼠学习记忆能力的影响[J]. 解剖学报. 2021, 52(3): 365-369 https://doi.org/10.16098/j.issn.0529-1356.2021.03.006

WANG Yue-jing ZHANG Meng LANG Wei-ya ZHANG Hai-yan LIAN Jie FENG Hua-jie SUN Li-hui. Effects of up-regulation of zinc finger and BTB domain containing 20 expression on learning and memory of APPswe/PSE9 transgenic Alzheimer disease mice[J]. Acta Anatomica Sinica. 2021, 52(3): 365-369 https://doi.org/10.16098/j.issn.0529-1356.2021.03.006

中图分类号： R332.81

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