卵泡液代谢标志物与多囊卵巢综合征患者卵母细胞质量的相关性

黄剑磊; 陈书强; 王明; 李辰龙; 穆静; 刘丹; 孙惠君; 王晓红

doi:10.16098/j.issn.0529-1356.2021.02.020

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解剖学报 ›› 2021, Vol. 52 ›› Issue (2) : 289-294. DOI: 10.16098/j.issn.0529-1356.2021.02.020

组织学胚胎学发育生物学

卵泡液代谢标志物与多囊卵巢综合征患者卵母细胞质量的相关性

黄剑磊陈书强王明李辰龙穆静刘丹孙惠君王晓红*

作者信息 +

Correlation between follicular fluid metabolic markers and oocyte quality in patients with polycystic ovary syndrome

HUANG Jian-lei CHEN Shu-qiang WANG Ming LI Chen-long MU Jing LIU Dan SUN Hui-jun WANG Xiao-hong*

Author information +

文章历史 +

摘要

目的探讨多囊卵巢综合征（PCOS）患者卵泡液中的代谢产物能否作为预测卵母细胞质量和早期胚胎发育的指标因子。方法根据中国身体质量指数(BMI)分类标准，将研究对象分为4组：正常对照组（LC）30例，肥胖组（OW）13例，瘦型PCOS组（LP）26例和肥胖PCOS组（OP）26例。采用超高效液相色谱-质谱联用技术进行卵泡液代谢组学分析；通过单因素方差分析比较组间患者临床特征，卵母细胞结局和卵泡液代谢物差异；利用 Pearson 相关性分析，探讨代谢物与受精率和优胚率的关系。结果与LC组相比，OP组的获卵数和优胚率显著增加，卵母细胞成熟率和受精率显著降低。通过代谢组学检测到卵泡液中有236种代谢物，与LC组和OW组相比，PCOS患者组有19种代谢物存在差异。此外，分别有7种脂质代谢物与受精率、优胚率成显著相关性，其中大多为溶血磷脂类。结论 PCOS患者与正常女性相比有多种卵泡液代谢物存在差异。这些差异代谢物中溶血磷脂类是影响卵母细胞质量和早期胚胎发育的重要因素，可作为评估PCOS患者卵母细胞质量的潜在标志物。

Abstract

Objective To investigate whether the metabolites in follicular fluid can be used as indicators for predicting oocyte quality and embryos early development in patients with polycystic ovary syndrome(PCOS). Methods The study subjects were divided into four groups: lean control (LC) 30 cases, overweight (OW) 13 cases, lean PCOS (LP) 26 cases, and overweight PCOS (OP) 26 cases based on the Chinese criteria for body mass index (BMI) categories. The metabolic variance of follicular fluid from PCOS and controls was performed by liquid chromatography-mass spectrometry (LC-MS), and clinical characteristics, oocyte outcomes and differences of metabolites in follicular fluid of those patients have been compared by ANOVA. Furthermore, Pearson’s correlation analysis was used to explore the correlation between oocyte fertilization rate, top-quality embryos rate and follicular metabolites. Results Compared to the LC group, oocytes retrieved and top-quality embryos rate were significantly increased, while the mature oocytes rate and fertilization rate decreased significantly in the OP group. A total of 236 metabolites were identified and quantified by metabolomics in follicular fluid. Compared with LC and OP groups, 19 metabolites showed statistically significant differences in PCOS group. Additionally, 7 metabolites were significant correlated with the fertilization rate or top-quality embryo rate respectively, most of which were lysophospholipids. Conclusion Several metabolites in the follicular fluid are significantly different between PCOS and healthy women. Among these, lysophospholipids are crucial for oocyte quality and early embryonic development, may serve as potential markers to evaluate the oocyte quality in PCOS patients.

导出引用

黄剑磊陈书强王明李辰龙穆静刘丹孙惠君王晓红. 卵泡液代谢标志物与多囊卵巢综合征患者卵母细胞质量的相关性[J]. 解剖学报. 2021, 52(2): 289-294 https://doi.org/10.16098/j.issn.0529-1356.2021.02.020

HUANG Jian-lei CHEN Shu-qiang WANG Ming LI Chen-long MU Jing LIU Dan SUN Hui-jun WANG Xiao-hong. Correlation between follicular fluid metabolic markers and oocyte quality in patients with polycystic ovary syndrome[J]. Acta Anatomica Sinica. 2021, 52(2): 289-294 https://doi.org/10.16098/j.issn.0529-1356.2021.02.020

中图分类号： R715.9

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