心脉隆注射液改善大鼠离体心脏心肌缺血/再灌注损伤

王永洁; 姜永良; 林志; 刘燃; 姜雪梅; 陆地; 孙林

doi:10.16098/j.issn.0529-1356.2021.02.018

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解剖学报 ›› 2021, Vol. 52 ›› Issue (2) : 277-283. DOI: 10.16098/j.issn.0529-1356.2021.02.018

组织学胚胎学发育生物学

心脉隆注射液改善大鼠离体心脏心肌缺血/再灌注损伤

王永洁¹ 姜永良¹ 林志¹ 刘燃¹姜雪梅¹ 陆地^2* 孙林^1*

作者信息 +

Xinmailong injection improving myocardial ischemia reperfusion injury in isolated-heart of rats

WANG Yong-jie¹ JIANG Yong-liang¹ LIN Zhi¹ LIU Ran¹ JIANG Xue-mei¹ LU Di^2* SUN Lin^1*

Author information +

文章历史 +

摘要

目的探讨心脉隆注射液对大鼠离体心脏心肌缺血/再灌注（I/R）损伤的保护作用。方法 SPF级SD大鼠60只，随机分为6组，包括假手术(sham)组、I/R处理组以及心脉隆注射液处理组（50 mg/L、100 mg/L、200 mg/L、400 mg/L），每组10只；运用Langendorff灌流系统制作大鼠离体心脏I/R损伤模型，平衡时期持续灌注K-H液，离体心脏模型缺血复灌后灌注心脉隆注射液；多导生理转换仪Lab Chart 8.0数据采集分析软件，同步记录左心室发展压(LVDP)，描记左心室内压最大上升和下降速率(±dp/dtmax)以及心率(HR)。采用生化仪检测不同组冠状动脉流出液中心肌酶学，肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）、肌钙蛋白-Ⅰ（TnI）和肌红蛋白（MYO）；实时测量冠状动脉流量，运用TTC染色了解心肌梗死面积，采用Western blotting检测Bax、Bcl-2在各组中的表达。结果心脉隆注射液能够减轻心肌缺血再灌注损伤大鼠LVDP、最大上升速率+dp/dt、最大下降速率-dp/dt的下降，减少心肌缺血再灌注损伤大鼠心肌细胞酶的释放，减少心肌梗死面积，增加心脏冠状动脉流量，引起心率增快以及一定的抑制心肌凋亡作用。结论心脉隆注射液能够减轻心肌I/R损伤引起的心功能损害。

Abstract

Objective To observe the protective effect of Xinmailong injection (XML) on myocardial ischemia reperfusion (I/R) injury in rats model. Methods The levels of SPF 60 successful acute myocardial ischemia rat models were randomly divided into control group,I/R group and XML group(Different drug concentrations 50 mg/L,100 mg/L, 200 mg/L, 400 mg/L). The control group was continuously given K-H nutrient solution for 2 hours, the other group was given respective different drug concentrations or K-H nutrient solution by Langendorff isolated heart perfusion method after ischemia 30 minutes.The following indicators were detected: Hemodynamics included heart rate (HR), left ventricular development pressure (LVDP) and ±dp/dtmax; the myocardial enzymes included creatine kinase (CK), creatine minase-MB (CK-MB), troponin-Ⅰ (Tn-Ⅰ) and myoglobin (MYO), coronary flow and myocardial infarct size were observed. The expression of Bax and Bcl-2 in each group was detected by Western blotting and immunofluorescence. Results Xinmailong injection could alleviate LVDP and ±dp/dtmax; decrease the release of CK, CK-MB, Tn-Ⅰ, MYO and the shrink area of myocardial infarction; increase the coronary flow; enhance heart rate fast and certain inhibition of myocardial apoptosis. Conclusion Xinmailong injection can alleviate the damage of cardiac function caused by myocardial ischemia/reperfusion injury.

导出引用

王永洁姜永良林志刘燃姜雪梅陆地孙林. 心脉隆注射液改善大鼠离体心脏心肌缺血/再灌注损伤[J]. 解剖学报. 2021, 52(2): 277-283 https://doi.org/10.16098/j.issn.0529-1356.2021.02.018

WANG Yong-jie JIANG Yong-liang LIN Zhi LIU Ran JIANG Xue-mei LU Di SUN Lin. Xinmailong injection improving myocardial ischemia reperfusion injury in isolated-heart of rats[J]. Acta Anatomica Sinica. 2021, 52(2): 277-283 https://doi.org/10.16098/j.issn.0529-1356.2021.02.018

中图分类号： R541.4

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