血管紧张素受体1 相关受体蛋白在出生后小鼠耳蜗毛细胞和螺旋神经元内的动态表达

尹海燕; 王择雨; 郝长江; 张慧; 王凤琴; 李秀国; 陈京

doi:10.16098/j.issn.0529-1356.2021.02.003

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解剖学报 ›› 2021, Vol. 52 ›› Issue (2) : 175-181. DOI: 10.16098/j.issn.0529-1356.2021.02.003

神经生物学

血管紧张素受体1 相关受体蛋白在出生后小鼠耳蜗毛细胞和螺旋神经元内的动态表达

尹海燕¹ 王择雨¹ 郝长江¹ 张慧¹王凤琴¹ 李秀国^2*陈京^3*

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Dynamic expression of putative receptor protein related to the angiotensin type 1 receptor in postnatal mouse cochlear hair cells and spiral ganglion neurons

YIN Hai-yan¹ WANG Ze-yu¹ HAO Chang-jiang¹ ZHANG Hui¹ WANG Feng-qin¹ LI Xiu-guo^2* CHEN Jing^3*

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摘要

目的探讨血管紧张素受体1 相关受体蛋白（APJ）在出生后C57BL/6小鼠耳蜗毛细胞和螺旋神经元成熟过程的时空表达规律。

方法分别取出生后7 d（P7）、P14、P28和出生后2月（P2M）时间点各11只C57BL/6小鼠的耳蜗组织， Real-time PCR、Western blotting和免疫荧光检测APJ在不同时间点耳蜗毛细胞和螺旋神经元表达。结果 Real-time PCR、Western blotting结果显示，APJ在P7～P2M的耳蜗组织内表达呈上升趋势；免疫荧光结果显示，APJ在P14小鼠毛细胞和螺旋神经元中的表达明显上升，并持续至P2M； P14小鼠APJ在毛细胞和螺旋神经元内的表达呈现出从底转到顶转的下降趋势。结论 APJ在出生后的小鼠耳蜗成熟过程中表现出独特的时空模式，可能参与耳蜗成熟和听力的形成和维持。

Abstract

Objective To study the temporal and spatial expressions of G protein-coupled receptor, putative receptor protein related to angiotensin type 1 receptor (APJ), in mammal cochlea postnatal development. Methods The cochlear tissues of each group 11 C57BL/6 mice at postnatal day 7 (P7), P14, P28 and postnatal month 2(P2M) were taken out under a stereo microscope. Real-time PCR, Western blotting and immunofluorescent staining were used to detect the expressions of APJ in hair cells and spiral ganglion neurons. Results The expression pattern of APJ in cochleae showed an upward trend during the period from P7 to P2M. The temporal expressions of APJ in hair cells and spiral ganglion neurons increased obviously at P14 and P2M. The spatial expression patterns of APJ in hair cells and spiral ganglion neurons followed a declined gradient from base turns to apex turns at P14. Conclusion APJ expression exhibits a specific spatial and temporal pattern during mouse cochlea postnatal development, and may play a role in cochleae maturation and hearing formation.

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Putative receptor protein related to angiotensin type 1 receptor / Hair cell / Spiral ganglion neuron / Immunofluorescence / Mouse

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尹海燕王择雨郝长江张慧王凤琴李秀国陈京. 血管紧张素受体1 相关受体蛋白在出生后小鼠耳蜗毛细胞和螺旋神经元内的动态表达[J]. 解剖学报. 2021, 52(2): 175-181 https://doi.org/10.16098/j.issn.0529-1356.2021.02.003

YIN Hai-yan WANG Ze-yu HAO Chang-jiang ZHANG Hui WANG Feng-qin LI Xiu-guo CHEN Jing. Dynamic expression of putative receptor protein related to the angiotensin type 1 receptor in postnatal mouse cochlear hair cells and spiral ganglion neurons[J]. Acta Anatomica Sinica. 2021, 52(2): 175-181 https://doi.org/10.16098/j.issn.0529-1356.2021.02.003

中图分类号： R329.4

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