微小RNA144-3p靶向作用于组蛋白去乙酰化酶2促进心肌细胞肥厚及心功能损伤

谭含璇; 龚芳

doi:10.16098/j.issn.0529-1356.2021.01.021

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解剖学报 ›› 2021, Vol. 52 ›› Issue (1) : 130-134. DOI: 10.16098/j.issn.0529-1356.2021.01.021

组织学胚胎学发育生物学

微小RNA144-3p靶向作用于组蛋白去乙酰化酶2促进心肌细胞肥厚及心功能损伤

谭含璇¹ 彭绍蓉¹黄蔡华¹ 姜红峰¹ 刘娟² 龚芳^2*

作者信息 +

MicroRNA144-3p targeting histone deacetylase 2 promoting cardiomyocyte hypertrophy and cardiac function damage

TAN Han-xuan¹ PENG Shao-rong¹ HUANG Cai-hua¹ JIANG Hong-feng¹ LIU Juan² GONG Fang^2*

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文章历史 +

摘要

目的探讨微小RNA（miR）144-3p促进心肌细胞肥厚及心功能损伤的作用及可能的机制。方法将45只C57BL/6小鼠随机分为对照组、心肌肥厚模型组及miR144-3p转染组，采用超声心动图检测3组小鼠心功能相关指标；实验结束后，处死小鼠，对心肌组织进行HE染色；采用Real-time PCR技术，检测各组小鼠心肌细胞中miR144-3p的表达差异；采用Western blotting检测心肌组织中脑钠肽（BNP）蛋白、抗核因子（ANF）蛋白、β-肌球蛋白重链（β-MHC）、肌动蛋白α1（Acta1）蛋白、组蛋白去乙酰化酶2（HDAC2）蛋白的表达水平。结果与对照组相比，模型组和转染组的左室射血分数（EF）、舒张期室间隔厚度（IVSd）、收缩期室间隔厚度（IVSs）、舒张期左室后壁厚度（IVPWd）、收缩期左室后壁厚度（IVPWs）、心重指数、左心指数明显较高，而收缩期左室内径（LVDs）、舒张期左室内径（LVDd）较低（P＜0.05），但模型组、转染组之间各项指标差异不明显（P＞0.05）。与对照组相比，模型组和转染组miR144-3p相对表达量明显升高，且转染组明显高于模型组（P＜0.05）。与对照组相比，模型组和转染组ANF、β-MHC、Acta1和HDAC2蛋白表达水平明显增高（P＜0.05）。结论 MiR144-3p可能通过上调HDAC2加重心肌肥厚及心功能损伤。

Abstract

Objective To investigate the role and possible mechanism of microRNA(miR)144-3p in promoting cardiomyocyte hypertrophy. Methods Forty-five C57BL/6 mice were divided into control group, myocardial hypertrophy model group (model group), and miR144-3p transfection group (transfection group) according to their transfection method. The cardiac function related indexes of three groups of mice were detected. HE staining was performed on mouse myocardial tissue.The expression of miR144-3p in mouse cardiomyocytes was detected by Real-time PCR. Antinuclear factor (ANF), β-myosin heavy chain (β-MHC), actin α1(Acta1) and histone deacetylase 2 (HDAC2) were detected by Western blotting in three groups. Results Compared with the control group, the interventricular septal thickness- diastolic（IVSd）, interventricular septal thickness-systolic（IVSs）, diastolic left ventricular posterior wall thickness（IVPWd）, systolic left ventricular posterior wall thickness（IVPWs）, ejection fraction（EF）, cardiac weight index and left cardiac index of the model group and the transfection group were significantly higher, while systolic left ventricular diameter（LVDs）and diastolic left ventricular diameter（LVDd）were lower (P<0.05), but there was no significant difference between the model group and the transfection group(P>0.05). Compared with the control group, the relative expression of miR144-3p in the model group and the transfection group was significantly higher than that in the model group (P<0.05). Compared with the control group, the expression levels of antinuclear factor, β-myosin heavy chain, Actinα1 and histone deacetylase 2 in the model group and the transfected group were significantly higher (P<0.05). Conclusion miR144-3p can aggravate cardiac hypertrophy by up-regulating HDAC2 and is expected to become a new therapeutic target.

导出引用

谭含璇彭绍蓉黄蔡华姜红峰刘娟龚芳. 微小RNA144-3p靶向作用于组蛋白去乙酰化酶2促进心肌细胞肥厚及心功能损伤[J]. 解剖学报. 2021, 52(1): 130-134 https://doi.org/10.16098/j.issn.0529-1356.2021.01.021

TAN Han-xuan PENG Shao-rong HUANG Cai-hua JIANG Hong-feng LIU Juan GONG Fang. MicroRNA144-3p targeting histone deacetylase 2 promoting cardiomyocyte hypertrophy and cardiac function damage[J]. Acta Anatomica Sinica. 2021, 52(1): 130-134 https://doi.org/10.16098/j.issn.0529-1356.2021.01.021

中图分类号： R363.1

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