转化生长因子β信号通路对卵泡刺激素调节卵巢颗粒细胞功能的影响

王雨桐; 卫晓红; 葛玲; 杨云风; 徐健

doi:10.16098/j.issn.0529-1356.2021.01.019

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解剖学报 ›› 2021, Vol. 52 ›› Issue (1) : 118-123. DOI: 10.16098/j.issn.0529-1356.2021.01.019

组织学胚胎学发育生物学

转化生长因子β信号通路对卵泡刺激素调节卵巢颗粒细胞功能的影响

王雨桐卫晓红葛玲杨云风徐健*

作者信息 +

Transforming growth factor β signaling pathway regulating the function of follicle stimulating hormone in ovarian granulosa cells

WANG Yu-tong WEI Xiao-hong GE Ling YANG Yun-feng XU Jian*

Author information +

文章历史 +

摘要

目的探讨大鼠卵巢颗粒细胞中卵泡刺激素（FSH）和转化生长因子β（TGF-β）信号通路之间的相互作用。方法取21 d SD大鼠卵泡分离的颗粒细胞原代培养，实验分为对照组、FSH处理组和转化生长因子β Ⅱ型受体(TGF-β RⅡ）中和组。通过免疫细胞化学和 Western blotting定位和检测TGF-β RⅡ以及增殖细胞核抗原（PCNA）的表达，通过流式细胞术(FCM)评估细胞增殖、细胞周期变化和凋亡细胞的百分比，并通过ELISA测定颗粒细胞分泌的雌二醇(E2)水平。结果当颗粒细胞的TGF-β信号通路受到抑制时，FSH促进颗粒细胞PCNA的表达，增加增殖指数，调节细胞周期以及抑制颗粒细胞凋亡的功能明显减弱(P<0.05）；FSH刺激颗粒细胞雌激素分泌的作用未明显受到TGF-β信号通路抑制的影响（P>0.05）。结论 FSH对卵巢颗粒细胞的调节作用部分受TGF-β信号通路的影响。

Abstract

Objective To explore the interaction between follicle stimulating hormone (FSH) and transforming growth factor Beta (TGF-β) signaling pathway in rat ovarian granulosa cells. Methods The granulosa cells isolated from the follicles of 21 days SD rats. The experiments were divided into three groups: control group, FSH treated group and transforming growth factor beta receptor Ⅱ (TGF-β RⅡ) neutralizated group. Immunocytochemistry (ICC) and Western blotting were then used to locate and detect the expression level of TGF-β RⅡ and proliferating cell nuclear antigen (PCNA). The proliferation index (PI), cell cycle and percentage of apoptotic cells were assessed by flow cytometry (FCM), and the level of estradiol (E2) was determined by ELISA. Results FSH increased the expression of PCNA and PI, changed the cell cycle and inhibited apoptosis of GCs, and these actions were reduced significantly when TGF-βsignaling pathway was inhibited(P<0.05); FSH stimulated the secretion of E2, and the increase in E2 levels was not regulated by inhibition of the TGF-β signaling pathway(P>0.05). Conclusion The effects of FSH on ovarian granulosa cells are partly affected by the TGF-β signaling pathway.

导出引用

王雨桐卫晓红葛玲杨云风徐健. 转化生长因子β信号通路对卵泡刺激素调节卵巢颗粒细胞功能的影响[J]. 解剖学报. 2021, 52(1): 118-123 https://doi.org/10.16098/j.issn.0529-1356.2021.01.019

WANG Yu-tong WEI Xiao-hong GE Ling YANG Yun-feng XU Jian. Transforming growth factor β signaling pathway regulating the function of follicle stimulating hormone in ovarian granulosa cells[J]. Acta Anatomica Sinica. 2021, 52(1): 118-123 https://doi.org/10.16098/j.issn.0529-1356.2021.01.019

中图分类号： R322

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