微小RNA-513c-5p在宫颈癌中的表达及靶向组蛋白去乙酰化酶1调节宫颈癌细胞迁移和侵袭的机制

王丽娜; 崔娜

doi:10.16098/j.issn.0529-1356.2021.01.011

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解剖学报 ›› 2021, Vol. 52 ›› Issue (1) : 73-77. DOI: 10.16098/j.issn.0529-1356.2021.01.011

肿瘤生物学

微小RNA-513c-5p在宫颈癌中的表达及靶向组蛋白去乙酰化酶1调节宫颈癌细胞迁移和侵袭的机制

王丽娜¹ 刘永娟¹ 张莹¹刘荣霞¹梁珊¹ 宋春红²崔娜^3*

作者信息 +

MicroRNA-513c-5p expression in cervical cancer and targeting histone deacetylase 1 regulating cervical cancer cell migration and invasion

WANG Li-na¹ LIU Yong-juan¹ ZHANG Ying¹ LIU Rong-xia¹ LIANG Shan¹ SONG Chun-hong² CUI Na^3*

Author information +

文章历史 +

摘要

目的探讨微小RNA（miR)-513c-5p在宫颈癌中的表达及靶向组蛋白去乙酰化酶1（HDAC1）调节宫颈癌细胞迁移和侵袭的机制。方法临床收集宫颈癌患者86例，通过Real-time PCR检测肿瘤组织和癌旁组织中miR-513c-5p水平，分析其与宫颈癌病理特征的关系。通过双荧光素酶报告验证miR-513c-5p靶向HDAC1。将宫颈癌HeLa细胞系分为4组：对照组、类似物(mimic)组、mimic+HDAC1组和HDAC1组。通过质粒转染技术过表达miR-513c-5p和(或)HDAC1。Real-time PCR和Western blotting分别用于检测RNA或蛋白的表达水平。分别通过CCK-8法、细胞划痕实验和Transwell实验检测各组的细胞生长、迁移和侵袭能力。结果宫颈癌组织中miR-513c-5p水平显著低于癌旁组织。低水平的miR-513c-5p与更高的局部侵袭、淋巴转移和远端转移有关（P<0.05）。miR-513-5p靶向抑制HDAC1表达。过表达miR-513c-5p显著抑制宫颈癌细胞生长、迁移和侵袭（P<0.05）。过表达HDAC1促进细胞生长、迁移和侵袭（P<0.05），并且可以逆转miR-513c-5p的抑制作用（P<0.05）。结论低水平的miR-513c-5p可能与宫颈癌转移有关，并且miR-513c-5p可通过靶向抑制HDAC1蛋白的表达抑制宫颈癌HeLa细胞生长、迁移和侵袭。

Abstract

Objective To investigate the expression of microRNA (miR)-513c-5p in cervical cancer and the mechanism of targeting histone deacetylase 1 (HDAC1) regulating cervical cancer cell migration and invasion. Methods Clinically collected 86 patients with cervical cancer. The levels of miR-513c-5p in tumor tissues and adjacent tissues were detected by Real-time PCR. The relationship between miR-513c-5p and pathological characteristics of cervical cancer was analyzed. It was verified that miR-513c-5p targets HDAC1 by a dual luciferase report. Cervical cancer HeLa cells were divided into four groups: control group, mimic group, mimic+HDAC1 group and HDAC1 group. MiR-513c-5p and(or) HDAC1 were overexpressed by plasmid transfection technology. Real-time PCR and Western blotting were used to detect the expression level of RNA or protein, respectively. The cell growth, migration, and invasion capabilities of each group were measured by CCK-8 method , cell scratch test, and Transwell test. Results The level of miR-513c-5p in cervical cancer tissues was significantly lower than that in adjacent tissues. Low levels of miR-513c-5p were associated with higher local invasion, lymphatic metastasis, and distal metastasis (P<0.05). MiR-513-5p targeted HDAC1 expression. Overexpression of miR-513c-5p inhibited significantly the growth, migration and invasion of cervical cancer cells (P<0.05). Overexpression of HDAC1 promoted growth, migration and invasion (P<0.05), and reversed the inhibitory effect of miR-513c-5p (P<0.05). Conclusion Low levels of miR-513c-5p might be related to cervical cancer metastasis, and miR-513c-5p could inhibit the growth, migration and invasion of cervical cancer HeLa cells by targeted inhibition of HDAC1 protein expression.

导出引用

王丽娜刘永娟张莹刘荣霞梁珊宋春红崔娜. 微小RNA-513c-5p在宫颈癌中的表达及靶向组蛋白去乙酰化酶1调节宫颈癌细胞迁移和侵袭的机制[J]. 解剖学报. 2021, 52(1): 73-77 https://doi.org/10.16098/j.issn.0529-1356.2021.01.011

WANG Li-na LIU Yong-juan ZHANG Ying LIU Rong-xia LIANG Shan SONG Chun-hong CUI Na. MicroRNA-513c-5p expression in cervical cancer and targeting histone deacetylase 1 regulating cervical cancer cell migration and invasion[J]. Acta Anatomica Sinica. 2021, 52(1): 73-77 https://doi.org/10.16098/j.issn.0529-1356.2021.01.011

中图分类号： R737.33

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