银杏叶提取物对青春期乏运动大鼠行为学的影响

程晶

doi:10.16098/j.issn.0529-1356.2021.01.005

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解剖学报 ›› 2021, Vol. 52 ›› Issue (1) : 36-40. DOI: 10.16098/j.issn.0529-1356.2021.01.005

神经生物学

银杏叶提取物对青春期乏运动大鼠行为学的影响

程晶¹ 游剑虹² 陈琳琳³李振喜^1*

作者信息 +

Effect of ginkgo biloba extract on behaviors in rat with adolescent sedentariness

CHENG Jing¹YOU Jian-hong² CHEN Lin-lin³ LI Zhen-xi^1*

Author information +

文章历史 +

摘要

目的探讨银杏叶提取物对青春期乏运动大鼠行为学的影响及其可能机制。方法将24只4周龄SD雄鼠随机分为正常对照组(NC）、乏运动组（AS）以及银杏叶提取物处理组（GBE），干预性饲养至8周龄，进行旷场实验及高架十字迷宫实验检测行为学改变。比色法检测脑组织匀浆丙二醛（MDA）含量及超氧化物歧化酶(SOD)活性。Western blotting 检测脑实质糖原合成激酶3β(GSK-3β)和 β-连环蛋白(β-catenin)蛋白表达水平。结果旷场实验显示，AS组大鼠自主活动增加，而在旷场中央区域停留时间及次数减少；高架十字迷宫实验显示，AS组大鼠进入开臂时间及次数均减少。GBE干预有效逆转AS诱导的焦虑样行为（P＜0.05）。与NC组相比，AS组GSK-3β 和β-catenin比值上调，MDA含量增加，SOD活性降低；而GBE组GSK-3β和β-catenin比值明显下调，MDA含量下降，SOD活性增加（P＜0.05）。结论银杏叶提取物对青春期乏运动大鼠焦虑样行为具有改善作用，Wnt/β-catenin 信号通路及抗氧化调节可能参与脑保护作用。

Abstract

Objective To investigate the effect of Ginkgo biloba extract on behaviors in rat with adolescent sedentariness and its possible mechanism. Methods Twenty-four male SD rats (4-week-old) were randomly divided into normal control (NC), adolescent sedentarines (AS), and Ginkgo biloba extract (GBE) groups. After 4 weeks intervention with GBE, open-field test and elevated plus-maze test were performed to detect the behavioral changes. The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD)in brain were determined by colorimetric method. Protein levels of glycogen synthase kinase 3β(GSK-3β) and β-catenin in cerebral white matter were determined by Western blotting. ResultsIn open-field test, it was shown that the autonomic activity of rats in AS group increased, while the central regional travel time was reduced. Duration and number of residence in the open arm of elevated plus-maze test decreased in AS group. These anxiety-like behaviors were ameliorated by GBE intervention. Compared with the NC group, GSK-3β/β-catenin ratio and the content of MDA was upregulated in AS group while downregulated in GBE group. And activity of SOD were downregulated in AS group while significantly upregulated in GBE group (P<0.05). Conclusion Ginkgo biloba extract ameliorate anxiety-like behavior in rat with adolescent sedentariness. Wnt/β-catenin pathway and antioxidant regulation may play a cerebral protective role.

导出引用

程晶游剑虹陈琳琳李振喜. 银杏叶提取物对青春期乏运动大鼠行为学的影响[J]. 解剖学报. 2021, 52(1): 36-40 https://doi.org/10.16098/j.issn.0529-1356.2021.01.005

CHENG Jing YOU Jian-hong CHEN Lin-lin LI Zhen-xi. Effect of ginkgo biloba extract on behaviors in rat with adolescent sedentariness[J]. Acta Anatomica Sinica. 2021, 52(1): 36-40 https://doi.org/10.16098/j.issn.0529-1356.2021.01.005

中图分类号： R741.05，R964

参考文献

［1］ Dunstan DW, Howard B, Healy GN, et al. Too much sitting- -a health hazard ［J］. Diabetes Res Clin Pract, 2012, 97(3):368-376.

［2］ Scarborough P, Bhatnagar P, Wickramasinghe KK, et al. The economic burden of ill health due to diet, physical inactivity, smoking, alcohol and obesity in the UK: an update to 200607 NHS costs ［J］. J Public Health (Oxf), 2011,33(4):527-535.

［3］ Montes P, Ruiz-Sanchez E, Rojas C, et al. Ginkgo biloba extract 761: a review of basic studies and potential clinical use in psychiatric disorders［J］. CNS Neurol Disord Drug Targets, 2015, 14(1): 132-149.

［4］ Singh SK, Srivastav S, Castellani RJ, et al. Neuroprotective and antioxidant effect of ginkgo biloba extract against ad and other neurological disorders ［J］. Neurotherapeutics, 2019, 16(3):666-674.

［5］ Rojas P, Serrano-García N, Medina-Campos ON, et al. Antidepressant-like effect of a Ginkgo biloba extract (EGb761) in the mouse forced swimming test: role of oxidative stress ［J］. Neurochem Int, 2011, 59(5):628-636.

［6］ Suvorava T, Lauer N, Kojda G. Physical inactivity causes endothelial dysfunction in healthy young mice ［J］. J Am Coll Cardiol, 2004, 44(6):1320-1327.

［7］ You JH, Zhuang YF, Cheng J, et al. Juvenile activity levels affect predisposition to metabolic syndrome induced by maternal hypoxia in male offspring rats ［J］. Int J Clin Exp Med, 2016,9(2):1422-1434.

［8］ Kaur S, Sharma N, Nehru B. Anti-inflammatory effects of Ginkgo biloba extract against trimethyltin-induced hippocampal neuronal injury ［J］. Inflammopharmacology, 2018, 26(1):87-104.

［9］ Sedentary Behaviour Research Network. Letter to the editor: standardized use of the terms “sedentary“and “sedentary behaviours”［J］. Appl Physiol Nutr Metab, 2012,37(3):540-542.

［10］ H?jbjerre L, Sonne MP, Alibegovic AC, et al. Impact of physical inactivity on adipose tissue low-grade inflammation in first-degree relatives of type 2 diabetic patients ［J］. Diabetes Care, 2011, 34(10):2265-2272.

［11］ You JH, Zhuang YF, Cheng J, et al. Effects of different exercise load on vascular endothelial function of rats and its ultrasonographic evaluation ［J］. Chinese Journal of Rehabilitation Medicine, 2014,34(5):500-504.（in Chinese）

游剑虹, 庄伊凡, 程晶, 等. 不同运动负荷对大鼠血管内皮功能的影响及其超声评估［J］.中国康复医学杂志, 2014,34(5):500-504.

［12］ Schaller M, Murray DR, Bangerter A. Implications of the behavioural immune system for social behaviour and human health in the modern world ［J］. Philos Trans R Soc Lond B Biol Sci, 2015, 370(1669): 20140105.

［13］ Yeh KY, Shou SS, Lin YX, et al. Effect of Ginkgo biloba extract on lipopolysaccharide-induced anhedonic depressive-like behavior in male rats ［J］. Phytother Res, 2015,29(2):260-266.

［14］ Chen HH, Liu AQ, Wang XCh, et al. Functional approach of gingko biloba extract to the improvement of learning and memory disturbance in dementia models of aged rats ［J］. Chinese Journal of Clinical Rehabilitation, 2015,44(9):176-178.（in Chinese）

陈红辉, 刘安求, 王小川, 等. 银杏叶提取物改善学习记忆障碍老龄大鼠痴呆模型的作用途径［J］. 中国临床康复，2015,44(9):176-178.

［15］ Tian XY, Gao YY, Zhang WB, et al. Effect of the combination of ginaton and rehabilitation exercise on mild cognitive impairment ［J］. Journal of Harbin Medical University,2010,44(1): 74-76.（in Chinese）

田晓彦，高玉艳，张伟兵，等. 银杏叶提取物配合康复锻炼能有效改善轻度认知障碍的临床疗效观察［J］. 哈尔滨医科大学学报，2010,44(1): 74-76.

［16］ Feng L. Effects of Ginkgo biloba Extract on the behavioral abnormalities of socially isolated mice ［J］. China Journal of Traditional Chinese Medicine and Pharmacy, 2014, 29 (6): 1838-1840.（in Chinese）

冯雷. 银杏提取物改善小鼠因社会隔离引起的行为变化［J］. 中华中医药杂志, 2014,29(6): 1838-1840.

［17］ Lin J, Wei L, Huang ZhM, et al. Effects of egb on the behavioral profiles and Na+-K+-ATPase activities of cerebral cortex and hippocampus in the diabetic rats ［J］. Journal of Guangxi Medical University, 2004, 21(6):834-836.（in Chinese）

林军, 韦力, 黄志明. 银杏叶提取物对糖尿病大鼠行为学及大脑皮层和海马Na+-K+-ATP活性的影响［J］. 广西医科大学学报，2004, 21(6):834-836.

［18］ Ge JF, Xu YY, Qin G, et al. Resveratrol ameliorates the anxiety- and depression-like behavior of subclinical hypothyroidism rat: possible involvement of the HPT axis, HPA axis, and Wnt/β-catenin pathway ［J］. Front Endocrinol (Lausanne), 2016,7:44.

［19］ Crews F, He J, Hodge C. Adolescent cortical development: a critical period of vulnerability for addiction ［J］. Pharmacol Biochem Behav, 2007,86(2):189-199.

［20］ Egorov AV, Draguhn A. Development of coherent neuronal activity patterns in mammalian cortical networks: common principles and local hetereogeneity ［J］. Mech Dev, 2013, 130(6-8):412-423.