目的探讨母婴分离对母亲焦虑样行为的影响以及与雌激素受体α（ERα）和β（ERβ）相关的机制。 方法将30只C57BL/6 J母鼠分为对照组（CG，10只，不分离组）、短暂分离组（SG，10只，从分娩第2天～第10天母婴每天分离15 min）及长期分离组（LG，10只，从分娩第2天～第10天，母婴每天分离3 h）。通过旷场实验（OF）和高架十字实验（EPM）检测动物是否有焦虑样行为；通过免疫组织化学方法检测中脑区内侧视前区（mPOA）、下丘脑腹内侧核（VMH）和杏仁内侧核（MeA）ERα和ERβ免疫反应神经元（ERα-IRs和ERβ-IRs）的变化。 结果在OF中，LG组与CG组和SG组相比在旷场中心活动时间、穿格次数和总距离减少具有极显著性意义（P<0.001），SG组与CG组差异没有显著性（P>0.05）。在EPM中，LG组在开臂中活动时间占总时间百分比和运动距离极明显少于CG组和SG组（P<0.001），运动的总距离也比CG组和SG组明显减少（P<0.001）。免疫组织化学实验结果显示，LG组在mPOA、VMH和MeA 3个区域中ERα-IRs和ERβ-IRs明显低于CG组和SG组（P<0.001）。 结论母婴长期分离可能使母鼠产生焦虑样行为，而这一行为的产生可能与脑区ERα和ERβ显著减少有关。
Abstract
Objective To investigate the effects of dams-offspring separation on anxiety-like behaviors of dams, and if these anxiety-like behaviors of dams are associated with estrogen receptorα（ERα）and β（ERβ）in some brain regions. Methods Thirty C57BL/6 J female mice were divided into three groups, control group（CG，n=10，non-isolated group）, short-term separation group（SG，n=10, dams were separated from their offspring for 15 minuts per day from the second day to the tenth day after childbirth）and long-term separation group（LG，n=10, dams were separated from their offspring for 3 hours per day from the second day to the tenth day after childbirth）. Anxiety-like behaviors of dams were evaluated in an open-field（OF）and elevated plus-maze test（EPM）. The level of ERα- immunoreactive neurons（ERα-IRs）and ERβ- immunoreactive neurons（ERβ-IRs）in three brain regions including medial preoptic area（mPOA），hypothalamic ventromedial nucleus（VMH）and medial amygdaloid nucleus（MeA）were analyzed. Results In OF，compared to CG group and SG group, LG group had significantly less time in center area, crossing number and total distance（P<0.001），there were no significant difference between the CG group and SG group（P>0.05）. In EPM，compared to CG group and SG group，LG group had significantly less percentage of time, distance in open arms and total distance（P<0.001）. Compared to CG group and SG group，LG group had significantly less ERα-IRs and ERβ-IRs in mPOA, VMH，and MeA（P<0.01）. Conclusion Dams that are long-termly separated from their offspring may have anxiety-like behavior, and this behavior may be related to the significant reduction of ERα and ERβ in these brain regions.
关键词
焦虑样行为 /
雌激素受体α /
雌激素受体β /
旷场实验 /
高架十字实验 /
免疫组织化学 /
小鼠
Key words
Anxious-like behavior /
Estrogen receptor α /
Estrogen receptor β /
/
Open field test /
Elevated plus-maze test /
Immunohistochemistry /
Mouse
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基金
母婴分离影响母鼠产生抑郁样行为的雌激素机制;慢性不可预见温和刺激影响雌性小鼠抑郁和焦虑样行为的神经肽机制
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