中等强度运动对去卵巢大鼠骨质疏松及骨形态发生蛋白2信号通路的影响

张帅军

doi:10.16098/j.issn.0529-1356.2020.06.020

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解剖学报 ›› 2020, Vol. 51 ›› Issue (6) : 934-939. DOI: 10.16098/j.issn.0529-1356.2020.06.020

组织学胚胎学发育生物学

中等强度运动对去卵巢大鼠骨质疏松及骨形态发生蛋白2信号通路的影响

张帅军*

作者信息 +

Effects of moderate intensity exercise on osteoporosis and bone morphogenetic protein 2 signaling pathway in ovariectomized rats

ZHANG Shuai-jun*

Author information +

文章历史 +

摘要

目的探讨中等强度运动对去卵巢大鼠骨密度、骨质代谢、骨生物力学及骨形态发生蛋白2(BMP-2)信号通路的影响。方法将24只成年雌性SD大鼠随机分为假手术组、手术组与运动组，每组8只，假手术组仅切除双侧卵巢周围脂肪组织，手术组、运动组摘除双侧卵巢，摘除卵巢1周后运动组进行中等强度运动训练，共训练12周。12周后，进行股骨骨密度、生物力学、骨代谢、组织学检测，Western blotting检测股骨组织BMP-2、Runt相关转录因子2（Runx2）、Osterix及Smad1/5/8蛋白表达。结果手术组、运动组血钙、血磷、血抗酒石酸酸性磷酸酶(TRACP)浓度高于假手术组(P<0.05)，而运动组上述指标低于手术组(P<0.05)。手术组和运动组骨密度低于假手术组(P<0.05)，而运动组高于手术组(P<0.05)。手术组和运动组骨组织生物力学性能低于假手术组(P<0.05)，而运动组高于手术组(P<0.05)。组织学显示，运动组骨质疏松程度轻于手术组，骨组织内BMP-2表达量多于手术组。手术组和运动组BMP-2、Runx2、Osterix及Smad1/5/8蛋白表达低于假手术组(P<0.05)，而运动组上述蛋白表达高于手术组(P<0.05)。结论中等强度运动可改善去卵巢大鼠股骨的骨密度、生物力学性能与骨代谢，这一作用可能与BMP-2信号通路有关。

Abstract

Objective To investigate the effects of moderate intensity exercise on bone mineral density, bone metabolism, bone biomechanics and bone morphogenetic protein 2(BMP-2) signal pathway in ovariectomized rats. Methods Twenty-four adult female SD rats were randomly divided into sham operation group, operation group and exercise group with 8 rats in each group. The sham operation group only had bilateral peri-ovarian adipose tissue excised, while the operation group and the exercise group had bilateral ovaries excised. One week after ovariectomy, the exercise group received moderate intensity exercise training for 12 weeks. After 12 weeks, bone mineral density（BMD）, biomechanics, bone metabolism and histology of femur were measured, and the expression of BMP-2, Runt-related transcription factor 2(Runx2), Osterix and SMAD1 / 5 / 8 in femur were detected by Western blotting. Results The levels of serum calcium, phosphorus and tartrate resistant acid phosphatase in operation group and exercise group were higher than those in sham operation group (P<0.05), while those in exercise group were lower than those in operation group (P<0.05).BMD in operation group and exercise group was lower than that in sham operation group (P<0.05), while that in exercise group was higher than that in operation group(P<0.05).The biomechanical properties of bone tissue in operation group and exercise group were lower than that in sham operation group(P<0.05), while that in exercise group was higher than that in operation group(P<0.05).Histology showed that the degree of osteoporosis in exercise group was less than that in operation group, and the expression of BMP-2 in bone tissue was more than that in operation group. The expression of BMP-2, Runx2, Osterix and SMAD1/5/8 in operation group and exercise group was lower than that in sham operation group (P<0.05) but the expression of these proteins in exercise group was higher than that in operation group. Conclusion Moderate intensity exercise can improve bone mineral density, biomechanical properties and bone metabolism of femur in ovariectomized rats, which may be related to BMP-2 signaling pathway.

导出引用

张帅军. 中等强度运动对去卵巢大鼠骨质疏松及骨形态发生蛋白2信号通路的影响[J]. 解剖学报. 2020, 51(6): 934-939 https://doi.org/10.16098/j.issn.0529-1356.2020.06.020

ZHANG Shuai-jun. Effects of moderate intensity exercise on osteoporosis and bone morphogenetic protein 2 signaling pathway in ovariectomized rats[J]. Acta Anatomica Sinica. 2020, 51(6): 934-939 https://doi.org/10.16098/j.issn.0529-1356.2020.06.020

中图分类号： Q591.8

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