淫黄葛复合剂对阿尔茨海默病小鼠行为学和海马解聚素金属蛋白酶10表达的影响

马冬雪; 高维娟; 贺小平; 许力军; 刘亚磊; 董贤慧

doi:10.16098/j.issn.0529-1356.2020.06.003

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解剖学报 ›› 2020, Vol. 51 ›› Issue (6) : 821-826. DOI: 10.16098/j.issn.0529-1356.2020.06.003

神经生物学

淫黄葛复合剂对阿尔茨海默病小鼠行为学和海马解聚素金属蛋白酶10表达的影响

马冬雪^1,2 高维娟²贺小平²许力军²刘亚磊² 董贤慧^2*

作者信息 +

Effect of Astragalus, Epimedium and Pueraria compound on Alzheimer’s disease mice behaviors and hippocampus disintegrin and metalloprotease 10 expression#br#

MA Dong-xue1^,2 GAO Wei-juan²HE Xiao-ping²XU Li-jun² LIU Ya-lei² DONG Xian-hui^2*

Author information +

文章历史 +

摘要

目的探讨淫羊藿、黄芪、葛根有效组分复方对阿尔茨海默病（AD）模型小鼠行为学和海马CA3区解聚素金属蛋白酶10（ADAM10）表达的影响。方法 10月的雄性APPswe/PS1dE9双转基因模型小鼠30只随机分为复方组、模型组和去铁斯诺(DFX)组，10月龄的雄性C57BL/6 J小鼠10只作为正常对照组。用药结束后，采用Morris水迷宫检测各组小鼠学习记忆能力，并于水迷宫后取出小鼠的脑组织，应用免疫荧光、Real-time PCR和Western blotting方法检测各组小鼠海马CA3区ADAM10的表达。结果水迷宫实验结果显示，与正常对照组小鼠相比，模型组小鼠的逃避潜伏期、游泳距离和游泳时间均明显延长(P＜0.05)，穿越平台区域次数和平台区域停留时间明显减少(P＜0.05)，首次穿越平台的逃避潜伏期明显延长(P＜0.05)；与模型组小鼠相比，复方组和DFX组逃避潜伏期、游泳距离和游泳时间明显缩短(P＜0.05)，穿越平台区域次数和平台区域停留时间明显增多(P＜0.05)，首次穿越平台的逃避潜伏期明显缩短；复方组和DFX组相比，逃避潜伏期、游泳距离和游泳时间及跨台次数差异无显著性 (P＞0.05)，穿越平台区域次数、平台区域停留时间和首次穿越平台的逃避潜伏期差异无显著性 (P＞0.05)。与正常对照组小鼠相比，模型组小鼠海马CA3区ADAM10的表达增高(P＜0.05)；与模型组相比，复方组和DFX组小鼠海马CA3区DAM10的表达降低(P＜0.05)；复方组和DFX组相比，ADAM10表达水平差异无显著性 (P＞0.05)。结论应用淫羊藿、黄芪、葛根有效组分复方可以改善APPswe/PS1dE9双转基因AD模型小鼠的学习记忆能力，其机制可能与下调ADAM10的表达减少神经元的损伤和死亡有关。

Abstract

Objective To investigate the effects of a combination of Astragalus, Epimedium and Pueraria effective extracts on behaviors and the expression of a disintegrin and metalloprotease 10 (ADAM10) in hippocampal CA3 region of APPswe/PS1dE9 double transgenic Alzheimer’s disease (AD) model mice. Methods Thirty 10-month-old male APPswe/PS1dE9 double transgenic model mice were randomly divided into compound group, model group and the deferox (DFX) group, and 10 age-matched male C57BL/6 J mice were used as normal controls. After the medication was over, the learning and memory ability of mice in each group was measured by Morris water maze, and the brain tissue was taken out after the water maze test. The expression of ADAM10 in hippocampal CA3 region of mice in each group was detected by immunofluorescence, Real-time PCR and Western blotting. Results Compared with the normal control group, the escape latency, the swimming distance and the swimming time of the model group mice were significantly prolonged (P<0.05),crossing-target number and target-quadrant abidance were significantly decreases (P<0.05)，escape latency for first crossing the platform was significantly prolonged(P<0.05)；The escape latency, swimming distance and swimming time of astragalus, epimedium and pueraria effective component compound group and DFX group were significantly shorter than those of APPswe/PS1dE9 double transgenic AD model mice (P<0.05). Crossing-target number and target-quadrant abidance were significantly prolonged (P<0.05)，escape latency for first crossing the platform was significantly decreases (P<0.05)；There was no significant differences in escape latency, swimming distance, swimming time and cross times between the compound group and the DFX group (P>0.05). Crossing-target number，target-quadrant abidance and escape latency for first crossing the platform wer e no significant difference. Compared with the normal control group mice, AD model group increased the expression of hippocampal CA3 area ADAM10 (P<0.05), and the expression of ADAM10 in the hippocampal CA3 region of the compound group and DFX group was lower than that of the model group (P<0.05), but there was no significant difference in the expression of ADAM10 between the compound group and the DFX group. Conclusion The effective components of Herba Epimedii, Astragalus membranaceus and Pueraria lobata could improve the learning and memory ability of APPswe/PS1dE9 double transgenic AD model mice, and the mechanism might be related to the down-regulation of ADAM10 expression and the reduction of neuron damage and death.

导出引用

马冬雪高维娟贺小平许力军刘亚磊董贤慧. 淫黄葛复合剂对阿尔茨海默病小鼠行为学和海马解聚素金属蛋白酶10表达的影响[J]. 解剖学报. 2020, 51(6): 821-826 https://doi.org/10.16098/j.issn.0529-1356.2020.06.003

MA Dong-xue GAO Wei-juan HE Xiao-ping XU Li-jun LIU Ya-lei DONG Xian-hui. Effect of Astragalus, Epimedium and Pueraria compound on Alzheimer’s disease mice behaviors and hippocampus disintegrin and metalloprotease 10 expression#br#[J]. Acta Anatomica Sinica. 2020, 51(6): 821-826 https://doi.org/10.16098/j.issn.0529-1356.2020.06.003

中图分类号： R741

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