目的 基于16S rRNA基因测序，探讨鱼藤酮诱导的帕金森病(PD）模型小鼠体内肠道微生物的变化。  方法 14只8周雄性C57BL/6J小鼠连续5周每天皮下注射鱼藤酮（3 mg/kg），每周测1次体重，5周后进行行为学检测，包括转棒实验和旷场实验，最后收集肠道内容物，用于肠道微生物检测分析。  结果 给鱼藤酮5周后，PD模型小鼠的体重显著低于对照小鼠（P<0.05），PD模型小鼠在转棒中的运动时间（P<0.0001）和在旷场中的运动总路程（P<0.05）均显著低于对照小鼠。PD模型小鼠肠道微生物Alpha多样性没有变化（P> 0.05），但是微生物物种与对照组间差异有显著性，其中PD模型小鼠肠道铜绿假单胞菌（Turicibacter）显著减少（P<0.01），毛螺旋菌科（norank_f_Lachnospiraceae）显著增多（P<0.01），丹毒丝菌科（norank_f_Erysipelotrichaceae）显著减少（P<0.01），腔隙杆菌属（Lachnoclostridium）显著增多（P<0.01）。 

 结论 PD模型小鼠的肠道微生物紊乱；肠道菌群紊乱可能参与PD模型小鼠运动障碍的发生。

Objective To investigate the changes of intestinal microbes in rotenone-induced Parkinson’s disease (PD) mice based on 16S rRNA gene sequencing.   Methods Fourteen 8-weekold male C57BL/6J mice were randomly divided into two groups: 6 mice in the control group and 8 mice in the model group. The model mice were injected subcutaneously with rotenone (3 mg/kg) for 5 weeks, and the body weight was measured once a week. After 5 weeks, behavioral tests were performed, including the rotating rod test and the open field test. The contents of the tract were used for intestinal microbial detection analysis.  Results After 5 weeks of rotenone treatment, the weight of PD mice was significantly lower than that of the control mice（P<0.05）. The movement time of the PD model mice in the rotating rod（P<0.0001）and the total distance of movement in the open field（P<0.05）were significantly lower than that of the control mice. In addition, the intestinal microbial diversity of the PD model mice did not change（P>0.05）,but the microbial species showed significant differences. Among them, the PD mice showed a significant decrease in the intestinal  Turicibacter（P<0.01）, a significant increase in norank_f_Lachnospiraceae（P<0.01）, a significant decrease in norank_f_ Erysipelotrichacea e（P<0.01）, and a significant increase in Lachnoclostridium（P<0.01）.  Conclusion Intestinal microbes in PD mice are disordered, and these intestinal flora may be involved in the development of dyskinesia in PD mice.