目的  探讨肥胖对小鼠脂肪中炎症因子分泌的影响及其作用的分子机制。  方法  随机选取20只Lepob/ob肥胖小鼠作为研究对象，同窝野生型C57BL/6 J小鼠作为对照组。测定小鼠体重、脂肪重量、血糖、葡萄糖耐量和胰岛素耐量；HE染色观察白色和褐色脂肪细胞的状态，并对脂肪细胞直径进行统计学分析；Western blotting检测诱导型一氧化氮合酶(iNOS)、核因子κB（NF-κB）、蛋白激酶B（Akt）和p-Akt的蛋白表达；Real-time PCR分析白色和褐色脂肪中CC-趋化因子配体2(CCL2)、CD44、集落刺激因子2(CSF2)、胶质纤维酸性蛋白（GFAP）、Iba1、白细胞介素(IL)-1α、IL-6、IL-7、JUN和S100β mRNA的表达。  结果  与对照组相比，Lepob/ob 小鼠的体重随年龄的增长而显著增加（P<0.001），白色脂肪重量、皮下脂肪重量及血糖显著升高（P<0.01，P<0.01，P<0.01），葡萄糖耐量较低（P<0.001）并产生胰岛素抵抗（P<0.001）；脂肪细胞直径显著增大，且各个脂肪组织均有巨噬细胞浸润灶出现；脂肪细胞内Janus蛋白酪氨酸激酶2(JAK2)、p-JAK2、iNOS、NF-κB、Akt和p-Akt蛋白表达均显著升高（P<0.05）；CCL2、CD44、IL-1α、IL-6、IL-7、JUN和S100β mRNA表达均显著升高。 结论  肥胖诱导小鼠脂肪组织中炎症因子显著表达，促使细胞分泌传导紊乱，导致炎症级联发生。

Objective  To investigate the effect of obesity on the expression of inflammatory factors, and to explore the molecular mechanism of inflammatory factors in the adipose tissue.   Methods  Twenty Lepob/ob mice were randomly selected as the research object, and the wild type C57BL/6 J mice of the same nest and age as the control group. The body weight, fat mass, blood glucose level, glucose tolerance and insulin tolerance were measured. HE staining was performed to examine the morphological changes in the both white and brown adipose tissues. Western blots were conducted to analyze the protein expression of inducible nitric oxide synthase (iNOS), nuclear factor kappa-B (NF-κB), protein kinase B (Akt) and p-Akt. Furthermore, Real\|time PCR was performed to measure the mRNA expression of CC chemokine Iigand 2 (CCL2), CD44, colony stimulating factor 2(CSF2), glial fibrillary acidic protein (GFAP), Iba1, interleukin (IL)-1α, IL-6, IL-7, JUN and S100β in different adipose tissues.   Results  Comparing to the control group, the body weight of Lepob/ob mice increased significantly (P<0.001). Both fat mass and blood glucose level increased significantly (P<0.01, P<0.01, P<0.01), the tolerance of glucose was low(P<0.001) and insulin resistance was produced (P<0.001). Histological analysis of adipose tissue from Lepob/ob mice revealed an increased lipid accumulation and diameter of adipocytes compared to wild type littermates. The macrophage infiltration was also observed in adipose tissues. The expression of Janus kinase (JAK2), p-JAK2, iNOS, NF-κB, Akt and p-Akt in adipocytes of Lepob/ob mice increased significantly (P<0.05), and the expression of CCL2, CD44, IL-1α, IL-6, IL-7, JUN and S100β in the Lepob/ob mice increased significantly.   Conclusion  Obesity induces the significant expression of inflammatory factors in adipose tissue, which leads to disorder of cell secretion and conduction, and ultimately results in inflammatory cascade.