雌二醇对雌性家兔胃窦肌电活动影响的数学模型构建与分析

赵建帅; 张柳凝; 张莹利; 张金; 杜宜楠; 郭雅茹; 李想; 武永杰; 徐永平

doi:10.16098/j.issn.0529-1356.2020.03.007

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解剖学报 ›› 2020, Vol. 51 ›› Issue (3) : 352-360. DOI: 10.16098/j.issn.0529-1356.2020.03.007

神经生物学

雌二醇对雌性家兔胃窦肌电活动影响的数学模型构建与分析

赵建帅¹ 张柳凝² 张莹利¹张金¹ 杜宜楠¹郭雅茹¹李想² 武永杰¹徐永平^1*

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Mathematical model construction and analysis of the effect of estradiol on the electrical activity of gastric antrum in female rabbits

ZHAO Jian-shuai¹ ZHANG Liu-ning² ZHANG Ying-li¹ ZHANG Jin¹ DU Yi-nan¹ GUO Ya-ru¹ LI Xiang² WU Yong-jie¹ XU Yong-ping^1*#br#

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摘要

目的通过观察雌激素受体在胃窦的表达，并利用构建的数学模型研究雌激素对雌兔胃窦平滑肌肌电活动的影响，探讨雌激素对胃动力的调节作用。方法应用免疫荧光技术检测雌激素受体在胃窦壁的表达；采用BL-420F生物监测仪监测：1. 比较假手术组与卵巢摘除组家兔胃窦肌电活动指数的差异，每组各3只; 2. 观察苯甲酸雌二醇剂量（0、0.1、0.15、0.2、0.25和0.3 mg/kg）对胃窦肌电活动的影响，每个剂量组各3只。构建数学模型，分析量效关系和作用机制。结果雌激素受体在胃窦壁的表达呈阴性；卵巢摘除后胃窦肌电活动指数极显著性降低（P<0.01）；随着雌二醇剂量的增加，胃窦肌电活动指数先增加，后降低。方差分析表明，相邻各组间胃窦肌电活动指数差异性显著（P<0.05）或极显著（P<0.01）。以雌二醇剂量为自变量x，胃窦肌电活动指数为因变量y，拟合得到函数：y=2.80 + 5.65 × exp{-0.5×［(x-0.159)/0.038]2}为高斯函数（R=0.9884，P>0.05），表明雌二醇对胃窦平滑肌的调控是基于胃窦自身内在调控机制的基础上进行的，且对其内在调控机制的影响巨大。结论胃窦壁上不存在雌激素受体；雌二醇与胃窦肌电活动指数的量效关系数学模型符合高斯函数模型特征，雌二醇对胃窦平滑肌的活动起到促进作用，且是基于对胃窦肌电活动内在调控机制的影响下间接实现的。

Abstract

Objective To investigate the expression of estrogen receptor in gastric antrum and the effect of estrogen on electrical activity of gastric antrum smooth muscle in female rabbits in virtue of the constructed mathematical model in order to explore the regulation of estrogen on gastric motility. Methods Using immunofluorescence to observe the expression of estrogen receptor in gastric antrum. Using BL-420F bio monitor to monitor: Comparing the difference of antral electromyography index between rabbits in ovariectomized group and rabbits in sham operating group; Observing the dose-response relationship between doses of estradiol (0,0.1,0.15,0.2,0.25 and 0.3 mg/kg). To construct the mathematical model, and to analyze the dose-response relationship and mechanism of action. Rresults The expression of estrogen receptor in the antral wall of the stomach was negative. The activity index of gastric antrum myoelectric activity was significantly decreased after ovarian ablation (P<0.01). With the increase of estradiol dose, the activity index of gastric antrum muscle increased and then decreased. Analysis of variance showed that the difference of antral electromyographic activity index between adjacent groups was significant (P<0.05) or extremely significant (P<0.01). Taking the estradiol dose as the indepe ndent variable x, the antral electromyography activity index was the dependent variable y, and the fitting wass obtained: y=2.80 + 5.65 × exp{ -0.5 × ［(x-0.159)/0.038 ］2}. It was a Gaussian function (R=0.9884, P>0.05), which indicated that the regulation of estradiol on gastric antral smooth muscle was based on the intrinsic regulation mechanism of gastric antrum itself, and had a great influence on its internal regulation mechanism. Conclusion The above results indicate that there is no estrogen receptor on the gastric antrum. and the mathematical model of the dose-effect relationship of estradiol on the electrical activity index of gastric antrum in our study accords with the characteristics of Gaussian function model, which indicates that estradiol promotes the activity of gastric antral smooth muscle and is based on the stomach. It shows indirect realization of the internal regulation mechanism of sinus myoelectric activity.

导出引用

赵建帅张柳凝张莹利张金杜宜楠郭雅茹李想武永杰徐永平. 雌二醇对雌性家兔胃窦肌电活动影响的数学模型构建与分析[J]. 解剖学报. 2020, 51(3): 352-360 https://doi.org/10.16098/j.issn.0529-1356.2020.03.007

ZHAO Jian-shuai ZHANG Liu-ning ZHANG Ying-li ZHANG Jin DU Yi-nan GUO Ya-ru LI Xiang WU Yong-jie XU Yong-ping. Mathematical model construction and analysis of the effect of estradiol on the electrical activity of gastric antrum in female rabbits[J]. Acta Anatomica Sinica. 2020, 51(3): 352-360 https://doi.org/10.16098/j.issn.0529-1356.2020.03.007

中图分类号： Q424

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