目的 采用十二烷基硫酸钠（SDS）浸泡联合平板振荡制备兔甲状腺脱细胞生物支架，并对脱细胞支架进行评估。方法 20只新西兰大白兔随机分为正常对照组和脱细胞组，每组10只，脱细胞组兔甲状腺组织浸泡于1%SDS溶液后放置于37.0 ℃恒温振荡器中振荡24 h，去离子水振荡清除支架内残留去细胞试剂及DNA成分。对支架进行HE染色、DNA含量检测、扫描电子显微镜检查以及免疫荧光染色进行鉴定。 结果 甲状腺脱细胞支架DNA含量显著低于正常甲状腺组织（P<0.01），DNA清除率达90%以上；经检测甲状腺脱细胞支架内无DNA成分残留，同时较为完整地保存了支架3D空间结构及蛋白成分。 结论 浸泡法联合平板振荡法可有效清除甲状腺细胞成分，较为完整地保留支架空间结构及蛋白成分。

Objective To prepare thyroid gland decullularized biologic scaffolds by soaking with rotating and to assess the decullularized scaffolds. Methods According to random number table method, twenty New Zealand white rabbits were divided into control group and decullularized group, 10 cases in each group. The thyroid glands in decullularized group were immersed in 1% SDS and rotated at 37 ℃ for 24 hours. All thyroid gland decullularized scaffolds were rotated in deionized water to wipe off the decullularized reagent and residual DNA. HE staining, DNA concentration examination, electron microscope analysis and immunofluorescence technique were performed to indentify the characteristics of decullularized scaffolds. Results Compared to control group, the DNA concentration in the decellularized scaffold decrease significantly (P<0.01), the removal rate of DNA exceeded 90%; there were no visible cell nuclei remained, the 3D space structure and the protein components preserved well. Conclusion Soaking and rotating method can effectively wipe off the cellular components from the thyroid gland tissue, and preserve the extracellular matrix and three-dimensional structure.