鞘内注射M_ERK2-shRNA腺病毒对糖尿病神经痛小鼠的影响

王月静 姚宏波 张萌 廉洁 张海燕 刘丹阳 李林 张宇华 李尽贺 孙丽慧*

doi:10.16098/j.issn.0529-1356.2019.05.004

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解剖学报 ›› 2019, Vol. 50 ›› Issue (5) : 565-569. DOI: 10.16098/j.issn.0529-1356.2019.05.004

神经生物学

鞘内注射M_ERK2-shRNA腺病毒对糖尿病神经痛小鼠的影响

王月静¹ 姚宏波¹ 张萌¹ 廉洁¹张海燕¹ 刘丹阳¹李林¹ 张宇华² 李尽贺² 孙丽慧^1*

作者信息 +

Effects of intrathecal injection of M_ERK2-shRNA adenovirus on diabetic neuropathic pain in mouse

WANG Yue-jing¹ YAO Hong-bo¹ ZHANG Meng¹ LIAN Jie¹ ZHANG Hai-yan¹ LIU Dan-yang¹LI Lin¹ZHANG Yu-hua² LI Jin-he² SUN Li-hui ^1*

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文章历史 +

摘要

目的探讨细胞外调节蛋白激酶2 (ERK2）在糖尿病神经病理性疼痛（DNP）小鼠模型中的作用机制。方法40只小鼠随机分4组：对照组，DNP组，siERK2-DNP组（鞘内注射M_ERK2-shRNA腺病毒），siRNA-DNP组（鞘内注射siRNA腺病毒）。模型制作成功后检测痛阈值；模型制作前、模型制作后2周、 4周及6周检测机械痛阈值；6周后取脊髓组织，利用Western blotting及免疫组织化学法检测脊髓后角ERK2及核因子κB (NFκB)的表达。结果与对照组比较，DNP组和siRNA-DNP组痛阈值显著降低， ERK2、NFκB的表达显著上调（P<0.05），siERK2-DNP组与对照组比较，痛阈值无显著变化， ERK2、NFκB的表达无显著差别（P>0.05）。结论下调ERK2可能是治疗糖尿病神经病理性疼痛的脊髓后角神经元损伤的方法之一。

Abstract

Objective To explore the mechanism of extracellular regulated protein kinase 2 (ERK2) in diabetic neuropathic pain(DNP) mice. Methods Forty mice were randomly divided into four groups: control group, DNP group, siERK2-DNP group (intrathecal injection of M_ERK2-shRNA adenovirus), siRNA-DNP group (intrathecal injection of siRNA adenovirus). The pain threshold was detected after the model made successfully, the mechanical pain threshold was detected before moulding, after moulding 2 weeks, 4 weeks and 6 weeks. Spinal cord tissues were taken after 6 weeks, and the expression of ERK2 and nuclear factor κB (NFκB) in the posterior horn of the spinal cord was detected by Western blotting and immunohistochemistry. Results Compared with the control group, the pain threshold of DNP group and siRNA-DNP group decreased significantly, the expression of ERK2 and NFκB increased significantly (P<0.05). Compared with the control group, the pain threshold of siERK2-DNP group had no significant change, and the expression of ERK2 and NFκB had no significant difference (P>0.05). Conclusion The marked down-regulation of ERK2 may be one of the important mechanisms affecting neuronal injury in the spinal dorsal horn of diabetic neuropathic pain.

导出引用

王月静姚宏波张萌廉洁张海燕刘丹阳李林张宇华李尽贺孙丽慧. 鞘内注射M_ERK2-shRNA腺病毒对糖尿病神经痛小鼠的影响[J]. 解剖学报. 2019, 50(5): 565-569 https://doi.org/10.16098/j.issn.0529-1356.2019.05.004

WANG Yue-jing YAO Hong-bo ZHANG Meng LIAN Jie ZHANG Hai-yan LIU Dan-yang LI Lin ZHANG Yu-hua LI Jin-he SUN Li-hui. Effects of intrathecal injection of M_ERK2-shRNA adenovirus on diabetic neuropathic pain in mouse[J]. Acta Anatomica Sinica. 2019, 50(5): 565-569 https://doi.org/10.16098/j.issn.0529-1356.2019.05.004

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