大鼠背根神经节中的Nogo-A蛋白促进微管聚合和炎症热痛觉敏化的发生

胡启国 刘怀存 陈玲 赵妍 王君* 

doi:10.16098/j.issn.0529-1356.2019.05.001

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解剖学报 ›› 2019, Vol. 50 ›› Issue (5) : 549-553. DOI: 10.16098/j.issn.0529-1356.2019.05.001

神经生物学

大鼠背根神经节中的Nogo-A蛋白促进微管聚合和炎症热痛觉敏化的发生

胡启国刘怀存陈玲赵妍王君*

作者信息 +

Nogo-A in the rat dorsal root ganglion promotes the polymerization of tubulin and inflammatory heat hyperalgesia

HU Qi-guo LIU Huai-cun CHEN Ling ZHAO Yan WANG Jun*

Author information +

文章历史 +

摘要

目的探讨背根神经节（DRG）神经元中的Nogo-A在炎症性热痛觉敏化过程中对微管的调控作用及其下游分子通路。方法对野生型大鼠(n=12)和Nogo-A敲除大鼠(n=14)足底注射完全弗式佐剂（CFA）制造炎症痛大鼠模型，取两组大鼠DRG组织，采用Western blotting、免疫荧光染色等方法，研究DRG中Nogo-A在炎症热痛觉敏化过程中对于微管的调节作用。结果 Nogo-A基因敲除具有缓解CFA诱导的炎症性热痛觉敏化作用，Nogo-A基因敲除大鼠DRG中的微管聚合减少，微管上游信号分子磷酸化脑衰反应调节蛋白2（p-CRMP2）表达增加。结论 Nogo-A通过增强CRMP2促进微管聚合，促进了炎症热痛觉敏化的发生。

Abstract

Objective To investigate the regulation of microtubules by Nogo-A in the dorsal root ganglia during the inflammatory pain. Methods The ipsilateral paw withdrawal latency（PWL）was measured in wild type rats（WT，n=12) and Nogo-A konck-out (Nogo-A KO) rats (n=14) after complete Freunds adjuvant (CFA) injection. Western blotting and immunofluorescent staining were used to study the expression of microtubules and phosphorylated collapsin response mediator protein 2（p-CRMP2）in the dorsal root ganglion (DRG) of both groups. Results Knock out of Nogo-A in rats had attenuated the CFA-induced inflammatory hyperalgesia. The acetylated tubulin was reduced, and the expression of p-CRMP2 was increased in the DRG of the Nogo-A KO rats. Conclusion Nogo-A is involved in the regulation of inflammatory heat hyperalgesia by promoting the microtubule polymerization via CRMP2 pathway.

导出引用

胡启国刘怀存陈玲赵妍王君. 大鼠背根神经节中的Nogo-A蛋白促进微管聚合和炎症热痛觉敏化的发生[J]. 解剖学报. 2019, 50(5): 549-553 https://doi.org/10.16098/j.issn.0529-1356.2019.05.001

HU Qi-guo LIU Huai-cun CHEN Ling ZHAO Yan WANG Jun. Nogo-A in the rat dorsal root ganglion promotes the polymerization of tubulin and inflammatory heat hyperalgesia[J]. Acta Anatomica Sinica. 2019, 50(5): 549-553 https://doi.org/10.16098/j.issn.0529-1356.2019.05.001

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