光照强度对促性腺激素释放激素在京红Ⅰ号蛋鸡中脑和间脑表达的影响

刘海斌 吴占福 张立永 官丽辉 陈宝江 胡满*

doi:10.16098/j.issn.0529-1356.2019.03.004

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解剖学报 ›› 2019, Vol. 50 ›› Issue (3) : 285-291. DOI: 10.16098/j.issn.0529-1356.2019.03.004

神经生物学

光照强度对促性腺激素释放激素在京红Ⅰ号蛋鸡中脑和间脑表达的影响

刘海斌^1,2 吴占福² 张立永² 官丽辉² 陈宝江¹ 胡满^1*

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Effect of light intensity on expression of gonadotropin-releasing hormone in mesencephalon and diencephalon of Jinghong-Ⅰhens

LIU Hai-bin^1,2 WUZhan-fu² ZHANG Li-yong² GUAN Li-hui² CHEN Bao-jiang¹ HU Man^1*

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摘要

目的探讨光照强度对影响蛋鸡繁殖性能的神经调节机制。方法选取100日龄京红Ⅰ号蛋鸡60只，随机分为4组，每组15只，每组重复3次，每次重复5只，分别接受光照强度30 lx、20 lx、10 lx和1 lx处理，用免疫组织化学方法和图像分析技术检测促性腺激素释放激素（GnRH）免疫反应阳性神经元在鸡中脑和间脑的表达和分布。结果 GnRH免疫反应阳性神经元主要见于中脑的视顶盖中央灰质层（SGC）、峡核大细胞部（Imc）、峡核小细胞部（Ipc）、顶盖前下核/顶盖前间下核（SP/IPS）、基底视束根核（nbor）和半月核（SLu）。在SGC、Imc、Ipc、SLu内，光照强度30 lx和20 lx组的免疫反应阳性神经元平均灰度值显著高于10 lx和1 lx组（P<0.05）；在SGC、Imc和Ipc内，30 lx组面积百分比最大，和20 lx相比差异不显著（P>0.05），显著高于10 lx和1 lx组（P<0.05）。GnRH免疫反应阳性神经元在间脑主要分布于圆核（Rot）、下丘脑室周核（PHN）、室旁核（PVN）、外侧膝状体腹侧核（GLv）、丘脑前背外侧核（DLAm）、皮质联合床核（CPa）、隔内侧核（SM）。GnRH免疫反应阳性神经元平均灰度值在Rot内，光照强度30 lx最高，显著大于20 lx、10 lx和1 lx组（P<0.05），在PHN、PVN、GLv内，30 lx和20 lx组相比差异不显著（P>0.05），均显著高于10 lx和1 lx组（P<0.05），面积百分比在Rot内，30 lx组最高，和20 lx组相比，差异不显著（P>0.05），显著大于10 lx和1 lx组（P<0.05），在PHN和PVN内，30 lx和20 lx显著大于10 lx和 1 lx（P<0.05）。结论光照强度显著影响GnRH在100日龄京红Ⅰ号蛋鸡中脑和间脑的表达，进而调节其生殖机能。光信息通路的离顶盖通路，包括侧枝循环-峡核回路在调节中起重要作用。

Abstract

Objcctive studies the effect of light intensity on expression of GnRH in Mesencephalon and Diencephalon of Jinghong-Ⅰhens. Method Jinghong-I hens aged 100d were randomly divided into four groups and applied a light of 30lx, 20lx, 10lx, and 1lx, respectively, to mensurate the distribution and expression of GnRH immunoreactive positive neurons in hen mesencephalon and diencephalon using immunohistochemical method. Results GnRH immunoreactive positive neurons were mainly observed in SGC, Imc, Ipc, SP/IPS, nbor, and SLu of mesencephalon. The mean gray values of positive neurons in 30lx and 20lx groups were significantly higher than those of 10lx and 1lx groups (p<0.05) in SGC, Imc, Ipc, and SLu. The percent cell area in SGC, Imc, and Ipc was the largest in 30 lx group, having no significant difference comparing 20lx, being significant higher than those of 10lx and 1lx (p<0.05). GnRH immunoreactive positive neurons were mainly observed in Rot, PHN, PVN, GLv, DLAm, CPa, and SM of diencephalon. The mean gray values of positive neurons in 30lx was the largest, being significantly higher than those of and 20lx, 10lx and 1lx groups (p<0.05) in Rot. There was no significant difference (p>0.05) between 30lx and 20lx group, but they were significant higher than those of 10lx and 1lx (p<0.05) in PHN, PVN, and GLv. The percent cell area in Rot was the largest in 30lx group, having no significant difference comparing 20lx, being significant higher than those of 10lx and 1lx (p<0.05). Those in 30lx and 20lx groups were significant higher than 10lx and 1lx (p<0.05) in PHN and PVN。Conclusion the expression of GnRH of 30lx and 20lx are higher significantly than 10lx and 1lx in hen mesencephalon and diencephalon.

关键词

光照强度 / 促性腺激素释放激素 / 中脑 / 间脑 / 免疫组织化学 / 京红Ⅰ号蛋鸡

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刘海斌吴占福张立永官丽辉陈宝江胡满. 光照强度对促性腺激素释放激素在京红Ⅰ号蛋鸡中脑和间脑表达的影响[J]. 解剖学报. 2019, 50(3): 285-291 https://doi.org/10.16098/j.issn.0529-1356.2019.03.004

LIU Hai-bin WUZhan-fu ZHANG Li-yong GUAN Li-hui CHEN Bao-jiang HU Man. Effect of light intensity on expression of gonadotropin-releasing hormone in mesencephalon and diencephalon of Jinghong-Ⅰhens[J]. Acta Anatomica Sinica. 2019, 50(3): 285-291 https://doi.org/10.16098/j.issn.0529-1356.2019.03.004

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