糖尿病下肢血管病变球囊扩张术后再狭窄大鼠模型构建

邹志伟 张倩 董建军* 

doi:10.16098/j.issn.0529-1356.2019.01.022

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解剖学报 ›› 2019, Vol. 50 ›› Issue (1) : 128-131. DOI: 10.16098/j.issn.0529-1356.2019.01.022

技术方法

糖尿病下肢血管病变球囊扩张术后再狭窄大鼠模型构建

邹志伟张倩董建军*

作者信息 +

Establishment of a diabetic lower extremity arterial disease rat model of restenosis after percutaneous transluminal angioplasty

ZOU Zhi-wei ZHANG Qian DONG Jian-jun*

Author information +

文章历史 +

摘要

目的探讨构建2型糖尿病（T2DM）下肢血管病变球囊扩张术（TPA）后再狭窄的SD大鼠模型的方法。方法 30只T2DM大鼠分为以下3组：动脉粥样硬化组（AS）、再狭窄组（RS）和对照组，HE染色观变血管形态及结构变化。α-平滑肌肌动蛋白（α-SMA）免疫组织化学染色对斑块组成成分进行检测。结果 RS组可见明显的内膜增生，形成再狭窄斑块。构成RS斑块的细胞主要是平滑肌细胞（SMCs）。结论采用球囊拉伤联合球囊扩张术成功构建了糖尿病大鼠下肢血管病变PTA后RS动物模型。

Abstract

Objective To establish an animal model of restenosis in diabetic lower extremity arterial disease rat after percutaneous transluminal angioplasty(PTA). Methods We divided diabetic rats(n=30) into three groups: atherosclerosis group(AS), restenosis group(RS) and control group. We examined the artery by hematoxylin and eosin(HE), and detected the plaque components by αsmooth muscle actin(α-SMA) immunohistochemical staining. Results Artery restenosis rat models were successfully developed. The smooth muscle cells (SMCs) were the key components of the RS plaque. Conclusion An experimental animal model of restenosis in diabetic lower extremity arterial disease rat after PTA is set up by the steps described in this study.

导出引用

邹志伟张倩董建军. 糖尿病下肢血管病变球囊扩张术后再狭窄大鼠模型构建[J]. 解剖学报. 2019, 50(1): 128-131 https://doi.org/10.16098/j.issn.0529-1356.2019.01.022

ZOU Zhi-wei ZHANG Qian DONG Jian-jun. Establishment of a diabetic lower extremity arterial disease rat model of restenosis after percutaneous transluminal angioplasty[J]. Acta Anatomica Sinica. 2019, 50(1): 128-131 https://doi.org/10.16098/j.issn.0529-1356.2019.01.022

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