目的 探讨Survivin对c-myc、核因子（NF）-κB基因的调控作用。 方法 shRNA干扰沉默食管癌ECA109细胞系中Survivin基因表达，观察c-myc、NF-κB基因的表达变化，流式细胞术检测细胞周期的改变。 结果 1. 食管癌ECA109细胞系中，Survivin shRNA干扰下调Survivin表达后，c-myc mRNA及蛋白的表达水平明显降低；2. Survivin表达后，NF-κB mRNA及NF-κB 总蛋白无明显变化，但其蛋白活化表达水平明显降低, NF-κB 上游调控核因子κB抑制物激酶（IKK）α、IKKβ mRNA表达下调；3. Survivin 表达下调导致G₂期延长，G₂期细胞比例增加，细胞增殖阻滞。 结论 Survivin在转录及蛋白水平对c-myc具有正向调控作用，Survivin表达下调抑制了IKKα、IKKβ mRNA的表达及NF-κB的磷酸化。

Objective To study the effect of Survivin shRNA interference on c-myc and NF-κB expression levels in esophageal cells ECA109. Methods Survivin shRNA interference technology was applied to liposomes transfection esophageal ECA109 cells. Half quantitative RT-PCR and Western blotting method were applied to detect genes expression of c-myc and NF-κB gene. Flow cytometry was used to detect the proliferation and apoptosis of cell lines index changes. Results 1. After Survivin shRNA transfected esophageal ECA109 cells, Survivin mRNA and protein expression obviously down-regulated, and c-myc mRNA and protein expression also down-regulated; 2. After Survivin down-regulated, NF-κB mRNA expression had no obvious change, however the phosphorylated proteins level of NF-κB was reduced, whose upstream factor nuclear factor kappa B inhibitor kinase predominate(IKK) alpha, IKK beta mRNA express was cut; 3. Esophageal cell ECA109 apoptosis increased significantly. Cells ratio increased in G₂ phase but decreased in S phase. The cell cycle blocked. Conclusion Survivin is effective on adjusting c-myc gene expression on mRNA and protein level. Survivin regulates NF-κB activation by down-regulation of IKK alpha, IKK beta mRNA expression.