海洛因对小鼠肾结构与功能的影响

何玲慧 陈诚 李重阳 俞诗源*

doi:10.16098/j.issn.0529-1356.2018.06.011

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解剖学报 ›› 2018, Vol. 49 ›› Issue (6) : 759-764. DOI: 10.16098/j.issn.0529-1356.2018.06.011

组织学胚胎学发育生物学

海洛因对小鼠肾结构与功能的影响

何玲慧¹陈诚¹李重阳²俞诗源^1*

作者信息 +

Effects of heroin on the renal tissue structures and functions of mice

HE Ling-hui¹CHEN Cheng¹LI Chong-yang² YU Shi-yuan^1*

Author information +

文章历史 +

摘要

目的探讨海洛因对小鼠肾组织的影响。方法将60只小鼠随机分为对照组和海洛因组，海洛因组在l～5 d、6～10 d、11～15 d用递增剂量(0.0571、0.0667、0.0706 g/kg)腹腔连续注射海洛因溶液15 d（每天两次，每次0.2 ml），对照组注射等量生理盐水，分别于给药5、10、15 d时检测小鼠体重和肾重的变化；用比色法检测血浆肌酐（Cr）、尿酸（UA）和肾组织丙二醛（MDA）含量及过氧化氢酶（CAT）、Na⁺-K^+-ATP酶、乳酸脱氢酶（LDH）活性的变化；用生物显微技术观察肾组织结构的变化；用免疫组织化学法检测肾组织水通道蛋白2（AQP2）表达的变化。结果与对照组相比，海洛因组小鼠体重、肾重及肾指数均降低，肾组织出现不同程度的损伤，肾小球固缩，肾小管上皮细胞肿胀、坏死、脱落，血浆Cr、UA含量升高（P<0.01），肾组织CAT、Na^+-K^+-ATP酶活性降低（P<0.01），LDH活性、MDA含量升高（P<0.05或P<0.01），AQP2蛋白的表达降低（P<0.01）。结论海洛因影响肾组织抗氧化能力，降低肾组织AQP2的表达，对肾组织结构与功能有明显的损伤效应。

Abstract

Objective To study the poisonous effects of heroin on the kidney tissues of mice. Methods Sixty mice were randomly divided into control group and heroin groups.The mice in heroin group were intraperitoneally injected with 0.2 ml heroin solution twice a day at incremental concentration doses 0.0571, 0.0667 and 0.0706 g/kg each concentration for 15 days.The mice in control group were injected saline at the same volume as that in heroin group.The body weight and kidney weight were weighted and the contents for catalase(Cr)and uric acid(UA)in plasma and contents for malondialdehyde (MDA)as well as the activities of catalase(CAT)，Na^+-K⁺-ATP and lactate dehydrogenase(LDH)in kidney tissues were respectively detected by colorimetry at day 5, day 10 and day 15 treatments.And the morphological structure of kidneys were observed under the microscopy.The immunohistochemiscal staining method was used to detect aquaporin 2 (AQP2) expression intensity. Results The body weight，kidney weight and kidney index of mice in the heroin group were decreased，compared with the mice in control group.And the morphological observation showed that glomerulus pykinosis，glomerulus solid，renal tubule epithelial cells swelling necrosis and fall up.The contents for Cr and UA in plasma were higher than those in control group（P<0.01）.The activity of CAT，Na^+-K^+-ATP decreased（P<0.01），the activity of LDH，the content of MDA were higher（P<0.05 or P<0.01）and the expression of AQP2 decreased（P<0.01）. Conclusion Heroin destroys the antioxidant capacity of the kidney tissue and reduces the expression of AQP2 in the kidney and the effect of heroin on serious damage of both renal tissues structure and functions.

导出引用

何玲慧陈诚李重阳俞诗源. 海洛因对小鼠肾结构与功能的影响[J]. 解剖学报. 2018, 49(6): 759-764 https://doi.org/10.16098/j.issn.0529-1356.2018.06.011

HE Ling-hui CHEN Cheng LI Chong-yang YU Shi-yuan. Effects of heroin on the renal tissue structures and functions of mice[J]. Acta Anatomica Sinica. 2018, 49(6): 759-764 https://doi.org/10.16098/j.issn.0529-1356.2018.06.011

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