半乳糖凝集素3低表达对人胃癌MGC-803细胞增殖的影响

吴雪艳 赵依纳 王小杰 李欣*

doi:10.16098/j.issn.0529-1356.2018.06.005

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解剖学报 ›› 2018, Vol. 49 ›› Issue (6) : 724-729. DOI: 10.16098/j.issn.0529-1356.2018.06.005

肿瘤生物学

半乳糖凝集素3低表达对人胃癌MGC-803细胞增殖的影响

吴雪艳赵依纳王小杰李欣*

作者信息 +

Influence of galectin-3 low expression on proliferation of MGC-803 cells

WU Xue-yan ZHAO Yi-na WANG Xiao-jie LI Xin*

Author information +

文章历史 +

摘要

目的探讨半乳糖凝集素3（galectin-3）表达抑制对人胃癌细胞系MGC-803细胞增殖产生的影响。方法将胃癌细胞系MGC-803细胞分为3组，siRNA干扰组（转染靶向galectin-3 的特异siRNA），空白对照组（只加转染试剂）和阴性对照组（转染非特异性的siRNA）。用Western blotting法检测转染后细胞中galectin-3蛋白的抑制效率，应用MTT法、集落形成实验检测galectin-3基因低表达对MGC-803细胞增殖的影响；用Western blotting和免疫组织化学法检测细胞周期蛋白D1（cyclin D1）和增殖细胞核抗原（PCNA）的表达情况。结果 siRNA干扰组细胞中galectin-3蛋白的表达与阴性对照组和空白对照组相比明显降低（P<0.05）；细胞集落形成实验和MTT显示，siRNA干扰组细胞的增殖能力与阴性对照和空白对照组细胞比较显著降低（P<0.05）；cyclin D1和PCNA蛋白表达均为siRNA 干扰组细胞表达显著低于两对照组（P<0.05）。结论 Galectin-3低表达可抑制胃癌MGC-803细胞的增殖能力，这可能与抑制cyclin D1和PCNA的表达有关。

Abstract

Objective To investigate the influence of galectin-3 suppression on the proliferation of human stomach carcinoma MGC-803 cells by down-regulating galectin-3 expression using RNA interference technique. Methods The MGC-803 cells were divided into three groups. The cells transfected with siRNA targeted galectin-3 was regarded as si-RNA interference group, the cells transfected with scrambled siRNA was taken for negative control and those with lipofectine TM2000 alone was blank control. Western blotting was applied to detect the suppression rate of galectin-3. The growth of the cells was assessed by MTT and colony formation assays. Western blotting and immunohistochemistry were used to detect the expression of cyclin D1 and proliferationg cell nuclear antigen(PCNA). Results The expression of galectin-3 was significantly inhibited in the experiment cells group when compared with those of the blank cells group and negative cells group (P<0.05). MTT and colony formation assays showed that proliferation of the experiment cells group decreased significantly (P<0.05). The expression of cyclin D1 and PCNA decreased compared with the negative control group and the blank control group (P<0.05). Conclusion Galectin-3 low expression inhibits the proliferation of MGC-803 cells which might associate with the suppression of cyclin D1 and PCNA expression.

导出引用

吴雪艳赵依纳王小杰李欣. 半乳糖凝集素3低表达对人胃癌MGC-803细胞增殖的影响[J]. 解剖学报. 2018, 49(6): 724-729 https://doi.org/10.16098/j.issn.0529-1356.2018.06.005

WU Xue-yan ZHAO Yi-na WANG Xiao-jie LI Xin. Influence of galectin-3 low expression on proliferation of MGC-803 cells[J]. Acta Anatomica Sinica. 2018, 49(6): 724-729 https://doi.org/10.16098/j.issn.0529-1356.2018.06.005

参考文献

［1］ Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015［J］.CA Cancer J Clin, 2016, 66(2): 115-132.

［2］ Li XL, Yu Q, Zhang XY. Research of galectin-3 in gastric carcinoma［J］. Chinese Journal of Gastroenterology and Mepatology, 2015, 24 (2): 220-222. (in Chinese)

李小林，余倩，张学彦. Galectin-3 在胃癌中的研究进展［J］. 胃肠病学和肝病学杂志, 2015, 24(2): 220-222.

［3］ Dumic J, Dabelic S, Flogel M. Galectin-3: an open-ended story ［J］. Biochim Biophys Acta, 2006, 1760(4): 616-635.

［4］ Mirandola L, Nguyen DD, Rahman RL, et al. Anti-galectin-3 therapy: a new chance for multiple myeloma and ovarian cancer ［J］? Int Rev Immunol, 2014, 33(5): 417-427.

［5］ Nio J, Takahashi lwanaga H, Morimatsu M, et al. Immunohistochemical and in situ hybridization analysis of galectin-3,a beta-galactoside binding lectin, in the urinary system of adult mice ［J］. Histochem Cell Biol, 2006, 126(1): 45-56.

［6］ Newlaczyl AU, Yu LG. Galectin-3 a jack of all trades in cancer ［J］. Cancer Lett, 2011, 313(2): 123-128.

［7］ Barrow H, Rhodes JM, Yu LG, et al. The role of galectins in colorectal cancer progression ［J］. Int J Cancer, 2011, 129(1):1-8.

［8］ Yan WZh, Dai J, Tian M, et al. Correlation between lymph node metastasis and the expression of galectin-3, CA19-9 of breast cancer research［J］. Chinese Journal of Laboratory Diagnosis, 2017, 21(9):1489-1491. (in Chinese)

颜文贞, 戴璟, 田猛, 等. 乳腺癌淋巴转移与galectin-3,CA19-9表达的相关性研究［J］. 中国实验诊断学, 2017, 21(9):1489-1491.

［9］ Xiang D, Luo Q. Effect of galectin-3 gene regulating Wnt/β-catenin signaling pathway on apoptosis of lung cancer cells ［J］. Chinese Journal of Gerontology, 2018, 38(2): 289-291. (in Chinese)

向丹, 罗琴. Galectin-3基因调控Wnt/β-catenin信号通路对肺癌细胞凋亡的影响［J］. 中国老年学杂志, 2018, 38(2): 289-291.

［10］ Jia LQ, Pan J, Li XQ. Different expression of galectin-3 and CK19 in benign and malignant follicular thyroid tumors by ultrasound-guided fine needle aspiration ［J］. Journal of China Clinic Medical Imaging, 2017, 28(1):19-22. (in Chinese)

贾丽琼, 潘炯, 李小强. 超声引导下穿刺细胞检测galectin-3和CK19在良、恶性甲状腺滤泡性肿瘤的表达差异［J］. 中国临床医学影像杂志, 2017, 28(1):19-22.

［11］ Miyazaki J, Hokari R, Kato S, et al. Increased expression of galectin-3 in primary gastric cancer and the metastatic lymph nodes ［J］. Oncol Rep, 2002, 9(6): 1307-1312.

［12］ Yang ZhM, Wu XT, He T, et al. Expression of galectin-3 mRNA in gastric cancer with Per-itoneal metastasis［J］. Journal of Sichuan University Ersity(Medical Science Edition), 2006, 37 (1):105-108. (in Chinese)

杨志明, 伍晓汀, 何涛, 等. 胃癌腹膜转移中galectin-3 mRNA 的表达与意义［J］. 四川大学学报(医学版), 2006, 37 (1):105-108

［13］ Shi YSh, Xu XY. The expression and its significance of galectin-3 and E-cadherin protein in gastric carcinoma［J］. China Modern Doctor, 2013, 51(24):86-88,91. (in Chinese)

施永胜, 徐小燕. Galectin-3 和E-cadherin 蛋白在胃癌中的表达及其意义［J］. 中国现代医生, 2013, 51(24):86-88,91.

［14］ Shi J, Liang YG. Effect of andrographolide on cell growth, apoptosis and expression of proliferating cell nuclear antigen protein in the human skin carcinoma A431 cell line［J］. Acta Anatomica Sinica, 2013, 44(1):73-78. (in Chinese)

石静, 梁勇刚. 穿心莲内酯对人皮肤基底细胞癌A431细胞生长、凋亡及增殖细胞核抗原蛋白表达的影响［J］. 解剖学报, 2013, 44(1):73-78.

［15］ Yang Y, Wei ShZh, Du YX, et al. Expression of P53,Ki-67 and PCNA in esophageal squamous cell carcinoma and correlation with radiotherapy effect and prognosis［J］. Journal of Southeast University (Medical Science Edition), 2017,36(2):155-161. (in Chinese)

杨艳, 韦淑贞, 杜云翔, 等. p53、Ki-67、PCNA的表达与食管鳞癌放疗近期疗效及预后的关系［J］. 东南大学学报(医学版), 2017, 36(2):155-161.

［16］ Matson JP, Cook JC. Coll cycle proliferation decisions: the impact of single cell analyses［J］. FFRB J, 2017, 284(3):362-375.

［17］ Coqueret O. Linking cyclins to transcription control［J］. Gene, 2002, 299(1-2): 35-55.

［18］ Yan WH, Zou K, He HB, et al. Effects of steroidal saponin RCE-4 from Radix australis on Ras/Erk and p16/cyclin D1/CDK4 pathways in human cervical cancer Caski cells［J］.Chinese Journal of Clinical Pharmacology and Therapentics, 2018, 26(3):247-254. (in Chinese)

颜为红, 邹坤, 贺海波, 等. 吉祥草中甾体皂苷RCE-4对人宫颈癌Caski细胞Ras/Erk和p16/cyclin D1/CDK4通路的影响［J］.中国临床药理学与治疗学, 2018, 26(3): 247-254.

［19］ Zhong M, Liu YX, Weng JH, et al. Expression and significance of Pin1 and Cyclin D1 in invasive ductal carcinoma of the breast ［J］. Shandong Medical Journal, 2017, 57(9):55-57. (in Chinese)

钟鸣, 刘英霞, 翁剑华, 等. 乳腺浸润性导管癌组织中Pin1、Cyclin D1蛋白表达变化及意义［J］. 山东医药, 2017, 57(9): 55-57.

［20］ Hu YP, Wu LL, Yang JJ. The Dys-expression of cortactin and Cyclin D1 in esophageal squamous cell carcinoma and clinicopatholopical correlation analysis ［J］. Chinese Journal of Laboratory Diagnosis, 2017, 21(11):1915-1919. (in Chinese)

胡艳萍, 吴林林, 杨建杰. Cortactin及Cyclin D1在食管鳞状细胞癌组织中的差异表达及临床病理相关性分析［J］.中国实验诊断学, 2017, 21(11): 1915-1919.

［21］ Huang ZCh, Chen Q, Weng XY, et al. Expression and clinical significance of pin1 and Cyclin D1 in gastrointestinal stromal tumor ［J］. Journal of China Medical University, 2017,46(6): 495-500. (in Chinese)

黄子成, 陈清, 翁晓源, 等. Pin1与Cyclin D1在胃肠间质瘤中的表达及临床意义［J］. 中国医科大学学报, 2017, 46(6):495-500.

［22］ Lu ML, Yan Ch, Lai D, et al. Cyclin D1 and cell cycle regulation ［J］. Biotechnology Bulletin, 2011,10(4):55-59. (in Chinese)

卢梦玲, 闫超, 赖多,等. Cyclin D1与细胞周期调控［J］. 生物技术通报, 2011,10(4):55-59.

［23］ Donnellan R, Chetty R. Cyclin D1 and human neoplasia ［J］. J Mol Part, 1998, 51:1-7.

［24］ Zhao XR, Deng L, Weng XX, et al. In-depth study of function and significance of cyclin D1 in nasopharyngeal carcinoma cell line ［J］. Chinese Journal of Oncology, 2001, 23(5): 373-375. (in Chinese)

赵晓荣, 邓琳, 翁新宪, 等. 周期蛋白D1 鼻咽癌细胞系中功能及意义的深入研究［J］.中华肿瘤杂志, 2001, 23(5): 373-375.