大鼠脑缺血再灌注后环氧合酶-2促进E-选择素表达增多及灯盏花素的脑保护作用

杨迎春 任占川* 陈新骥

doi:10.16098/j.issn.0529-1356.2018.06.001

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解剖学报 ›› 2018, Vol. 49 ›› Issue (6) : 703-707. DOI: 10.16098/j.issn.0529-1356.2018.06.001

神经生物学

大鼠脑缺血再灌注后环氧合酶-2促进E-选择素表达增多及灯盏花素的脑保护作用

杨迎春¹任占川^1* 陈新骥²

作者信息 +

Cyclooxygenase-2 promoting the expression of E-selectin and the cerebral protective effects of breviscapine after cerebral ischemic-reperfusion injury in rats

YANG Ying-chun¹ REN Zhan-chuan^1* CHEN Xin-ji²

Author information +

文章历史 +

摘要

目的观察大鼠脑缺血再灌后环氧合酶-2（COX-2）对E-选择素（E-selectin）表达的影响及灯盏花素对COX-2及E-selectin的影响，探讨脑缺血再灌注损伤后， COX-2加重脑损伤的机制及灯盏花素的脑保护作用。方法 48只清洁级SD大鼠随机分为假手术组、生理盐水组、SC-58125组、灯盏花素治疗组，在缺血2 h再灌注24 h时，神经功能学评分观察大鼠神经行为的变化，2,3,5-氯化三苯四氮唑（TTC）染色观察大鼠脑梗死灶体积的变化；免疫印迹法和酶联免疫吸附测定检测COX-2及E-selectin表达的变化。结果 SC-58125组和灯盏花素治疗组神经行为学评分明显降低，脑组织梗死体积显著减小，COX-2和E-selectin的表达明显下降。结论脑缺血再灌注后，COX-2可能通过促进E-selectin的表达加重缺血区的炎症反应；灯盏花素可能通过抑制COX-2及E-selectin的表达而发挥脑保护作用。

Abstract

Objective To investigate the mechanisms of cyclooxygenase-2 (COX-2) aggravating cerebral injury and the protective effects of breviscapine after cerebral ischemic-reperfusion injury, by observing the effect of COX-2 on the expression of E-selectin and the effect of breviscapine on the expression of COX-2 and E-selectin. Methods Forty-eight SD rats were randomly divided into sham group, saline group, SC-58125 group and breviscapine treatment group, at ischemia hour 2/reperfusion hour 24. Nerve function scores were used to observe the neurobehavioral changes of rats, 2,3,5-triphenyltetrazolium chloride (TTC) staining was used to assess the changes of cerebral infarction volume. Western blotting and enzyme-linked immunosorbent assay were used to detect the expression of COX-2 and E-selectin. Results In SC-58125 group and breviscapine treatment group, nerve function scores, cerebral infarction volume and COX-2 and E-selectinwere significantly decreased. Conclusion COX-2 aggravates inflammatory response of ischemia area by promoting the expression of E-selectin; breviscapine may play a protective role by inhibiting the expression of COX-2 and E-selectin.

导出引用

杨迎春任占川陈新骥. 大鼠脑缺血再灌注后环氧合酶-2促进E-选择素表达增多及灯盏花素的脑保护作用[J]. 解剖学报. 2018, 49(6): 703-707 https://doi.org/10.16098/j.issn.0529-1356.2018.06.001

YANG Ying-chun REN Zhan-chuan CHEN Xin-ji. Cyclooxygenase-2 promoting the expression of E-selectin and the cerebral protective effects of breviscapine after cerebral ischemic-reperfusion injury in rats[J]. Acta Anatomica Sinica. 2018, 49(6): 703-707 https://doi.org/10.16098/j.issn.0529-1356.2018.06.001

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