rDNA在细胞发育中的转录及调控

李佳 王琪 宮甜甜 张玥 单智焱* 雷蕾

doi:10.16098/j.issn.0529-1356.2018.05.023

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解剖学报 ›› 2018, Vol. 49 ›› Issue (5) : 695-698. DOI: 10.16098/j.issn.0529-1356.2018.05.023

综述

rDNA在细胞发育中的转录及调控

李佳王琪宮甜甜张玥单智焱* 雷蕾

作者信息 +

Transcription and regulation of rDNA in cell development

LI Jia WANG Qi GONG Tian-tian ZHANG Yue SHAN Zhi-yan* LEI Lei

Author information +

文章历史 +

摘要

细胞的生长发育受核糖体DNA(rDNA)和蛋白质基因的协同调控，细胞总RNA的85%是rDNA的转录产物。rDNA的转录改变会导致核糖体生物合成应激，影响细胞的生长、增殖和分化，导致疾病的发生。理解rDNA的转录调控有利于阐明细胞发育的机制，找到治疗疾病的新的作用靶点。我们综述了rDNA的结构及转录调控方式，阐述了rDNA在干细胞领域和疾病发生治疗中的作用，为rDNA的研究提供理论依据。

Abstract

The growth and development of cells are regulated by ribosome DNA(rDNA) and protein gene. Eighty-five percent of the total RNA is the transcription product of rDNA. Changes in rDNA transcription may lead to ribosomal biosynthesis stress, which affects cells growth, proliferation and differentiation, and further leads to the occurrence of diseases. Revealing the mechanism of rDNA transcriptional regulation is helpful to further elucidate the mechanism of cell development and find a new target for the treatment of diseases. In this paper, we describe the structure of rDNA and its transcriptional regulation,and discuss the roles of rDNA on the stem cells and occurring, treatment of disease, which provides a theoretical basis for rDNA in clinical research.

导出引用

李佳王琪宮甜甜张玥单智焱雷蕾. rDNA在细胞发育中的转录及调控[J]. 解剖学报. 2018, 49(5): 695-698 https://doi.org/10.16098/j.issn.0529-1356.2018.05.023

LI Jia WANG Qi GONG Tian-tian ZHANG Yue SHAN Zhi-yan LEI Lei. Transcription and regulation of rDNA in cell development[J]. Acta Anatomica Sinica. 2018, 49(5): 695-698 https://doi.org/10.16098/j.issn.0529-1356.2018.05.023

参考文献

［1］ Durut N1, SáezⅤásquez J2. Nucleolin: dual roles in rDNA chromatin transcription［J］. Gene, 2015, 556(1):7-12.

［2］ Cong R1, Das S, Ugrinova Ⅰ, et al. Interaction of nucleolin with ribosomal RNA genes and its role in RNA polymerase Ⅰ transcription［J］. Nucleic Acids Res, 2012, 40(19):9441-9454.

［3］ Goodfellow SJ, Zomerdijk JC. Basic mechanisms in RNA polymerase Ⅰ transcription of the ribosomal RNA genes［J］. Subcell Biochem, 2013, 61:211-236.

［4］ Diermeier SD, Németh A, Rehli M, et al. Chromatin-specific regulation of mammalian rDNA transcription by clustered TTF-Ⅰ binding sites［J］. PLoS Genet, 2013, 9(9):e1003786.

［5］ Tsekrekou M, Stratigi K, Chatzinikolaou G. The Nucleolus: in genome maintenance and repair［J］.Int J Mol Sci, 2017, 18(7):E1411.

［6］ Herdman C, Mars JC, Stefanovsky VY, et al. A unique enhancer boundary complex on the mouse ribosomal RNA genes persists after loss of Rrn3 or UBF and the inactivation of RNA polymerase Ⅰ transcription［J］. PLoS Genet, 2017 17, 13(7):e1006899.

［7］ Santoro R, Schmitz KM, Sandoval J, et al. Intergenic transcripts originating from a subclass of ribosomal DNA repeats silence ribosomal RNA genes in trans［J］. EMBO Rep, 2010, 11(1):52-58.

［8］ Zheng Z, Jia JL, Bou G, et al. rRNA genes are not fully activated in mouse somatic cell nuclear transfer embryos［J］. J Biol Chem, 2012,287(24):19949-19960.

［9］ Milhomen SS, Scacchetti PC, Pieczarka JC, et al. Are NORs always located on homeologous chromosomes? A FISH investigation with rDNA and whole chromosome probes in Gymnotus fishes (Gymnotiformes)［J］.PLoS One, 2013, 8(2):e55608.

［10］Zaidi SK, Boyd JR, Grandy RA, et al. Expression of ribosomal RNA and protein genes in human embryonic stem cells is associated with the activating H3K4me3 histone mark［J］. J Cell Physiol, 2016, 231(9):2007-2013.

［11］Watanabe-Susaki K, Takada H, Enomoto K, et al. Biosynthesis of ribosomal RNA in nucleoli regulates pluripotency and differentiation ability of pluripotent stem cells［J］. Stem Cells, 2014,32(12):3099-3111.

［12］Zhang Q, Shalaby NA, Buszczak M. Changes in rRNA transcription influence proliferation and cell fate within a stem cell lineage［J］. Science, 2014,343(6168):298-301.

［13］Simsek D, Tiu GC, Flynn RA, et al.The Mammalian Ribo-interactome reveals ribosome functional diversity and heterogeneity［J］. Cell, 2017,169 (6):1051-1065.

［14］Woolnough JL, Atwood BL, Liu Z, et al.The Regulation of rRNA gene transcription during directed differentiation of human embryonic stem cells［J］. PLoS One, 2016, 11 (6):e0157276.

［15］Zhao Q, Wu Y, Shan Z, et al. Serum starvation-induced cell cycle synchronization stimulated mouse rDNA transcription reactivation during somatic cell reprogramming into iPSCs［J］. Stem Cell Res Ther, 2016,7(1):112.

［16］Abdelmohsen K, Gorospe M.RNA-binding protein nucleolin in disease［J］.RNA Biol, 2012, 9 (6):799-808.

［17］Lin ZhX, Lü GZh, Zhou LX, et al. Activation of nucleoler rRNA genetranscription in lymphocytes by tumor promoters studies with in situ hybridization detected by fluorescent method［J］. Acta Anatomic Sinica, 1993, (3):291-296. (in Chinese)

林仲翔，吕桂芝，周立新，等. 促癌变剂对淋巴细胞核仁rRNA基因转录的活化作用——rDNA原位杂交荧光显示法［J］. 解剖学报，1993，（3）：291-296.

［18］Flach J, Bakker ST, Mohrin M, et al. Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells［J］. Nature, 2014, 512(7513):198-202.

［19］McKay SE, Yan W, Nouws J, et al.Auditory pathology in a transgenic mtTFB1 mouse model of mitochondrial deafness［J］.Am J Pathol, 2015, 185(12):3132-3140.

［20］Kovachev PS, Banerjee D, Rangel LP, et al. Distinct modulatory role of RNA in the aggregation of the tumor suppressor protein p53 core domain［J］. J Biol Chem, 2017, 292(22):9345-9357.

［21］Ye Q, Pang S, Zhang W, et al. Therapeutic targeting of RNA polymerase Ⅰ with the small-molecule CX-5461 for prevention of arterial injury-induced neointimal hyperplasia［J］. Arterioscler Thromb Vasc Biol, 2017,37(3):476-484.

［22］Bai D,Zhang J, Li T, et al. The ATPase hCINAP regulates 18S rRNA processing and is essential for embryogenesis and tumour growth［J］. Nat Commun, 2016, 7:12310.

［23］Liu B, Chen F, Wu Y, et al. Enhanced tumor growth inhibition by mesenchymal stem cells derived from iPSCs with targeted integration of interleukin24 into rDNA loci［J］. Oncotarget, 2017,8(25):40791-40803.

［24］Gayet-Ageron A, Lautenschlager S, Ninet B, et al. Sensitivity, specificity and likelihood ratios of PCR in the diagnosis of syphilis: a systematic review and meta-analysis［J］.Sex Transm Infect, 2013, 89(3):251-256.

［25］Tomkins A, Ahmad S, Cousins DE, et al. Screening for asymptomaticneurosyphilis in HIV patients aftertreatment of early syphilis: an observational study［J］. Sex Transm Infect, 2018, 94(5):337-339.