目的 探讨microRNA-613(miR-613)在人胶质瘤中的表达及其对增殖与转移的影响。 方法 采用Real-time PCR法对30例人胶质瘤患者瘤组织和30例非胶质瘤患者的脑组织的miR-613表达水平进行检测，并分析其与胶质瘤临床病理特征及预后的关系；采用LipofectamineTM2000将miR-613模拟物（miR-613 mimics）和无关序列对照（miR-613 con）转入U87细胞中，Real-time PCR检测两组细胞miR-613的表达水平，CCK8法检测细胞增殖变化，Transwell实验检测细胞侵袭和迁移能力的变化。 结果 miR-613在胶质瘤组织中表达低于正常的脑组织（P<0.05）。胶质瘤组织miR-613的表达水平与病理分级成负相关，与生存时间成正相关（P<0.05）；与miR-613 con比较，miR-613 mimics细胞增殖水平、侵袭与迁移能力显著降低（P<0.05）。 结论 miR-613具有抑制胶质瘤细胞的生长、侵袭与迁移的作用，有望成为胶质瘤的潜在治疗靶点。

Objective To investigate the expression of microRNA-613(miR-613) in human glioma and its effect on the proliferation and metastasis. Methods Thirty human glioma patients tissues and 30 Non-glioma patients tissueswere detected by Real-time PCR, and to analysie their relationship with clinicopathological characteristics of gliomas. MiR-613mimics and unrelated sequence control(miR-613 con) were transfected into U87 cells using Lipofectamine TM2000. The expression of miR-613 in the two groups of cells was detected by Real-time PCR, the proliferation of cells was detected by CCK8 assay, and the invasion and migration of cells were detected by Transwell assay. Results The expression of miR-613 in Glioma tissues was lower than that in corresponding paracancerous tissues (P<0.05). The expression level of miR-613 in glioma tissue was negatively correlated with pathological grade and positively correlated with survival time (P<0.05). Compared with miR-613 con cells, miR-613 mimics cells proliferation, invasion and migration were significantly decreased (P<0.05). Conclusion MiR-613 can inhibit the proliferation, invasion and migration of glioma cells, and it might be a potential therapeutic target for the treatment of glioma.