&nbsp;青海省妊娠高血压综合征患者瘦素基因启动子区G-2548 A单核苷酸的多态性

庄文婷 赵得雄 王香林 李红荣 李长兴 李福鑫 孔得霞 李建华*

doi:10.16098/j.issn.0529-1356.2018.04.018

PDF(266 KB)

解剖学报 ›› 2018, Vol. 49 ›› Issue (4) : 524-528. DOI: 10.16098/j.issn.0529-1356.2018.04.018

人类学

青海省妊娠高血压综合征患者瘦素基因启动子区G-2548 A单核苷酸的多态性

庄文婷¹赵得雄²王香林¹李红荣³李长兴¹ 李福鑫¹ 孔得霞⁴李建华^1*

作者信息 +

Polymorphism of leptin gene G-2548 A and pregnancy-induced hypertension syndrome in Han population of Qinghai Province

ZHUANG Wen-ting¹ ZHAO De-xiong² WANG Xiang-lin¹ LI Hong-rong³LI Chang-xing¹ LI Fu-xin¹ KONG De-xia⁴ LI Jian-hua ^1*

Author information +

文章历史 +

摘要

目的探讨青海省妊娠高血压综合征(PIH)与瘦素基因启动子区单核苷酸多态性的关系。方法选择青海省PIH患者130例，正常妊娠对照组135例，应用聚合酶链反应限制性内切酶片段长度多态性(PCR-RFLP)方法，分析PIH患者和对照组瘦素基因G2548 A多态性分型并测序验证。结果 PIH组基因型频率GG型、GA型和AA型分别为53.1%、40%和6.9%，对照组分别为60%、34.1%和5.9%。PIH组和对照组瘦素基因 G-2548 A 位点等位基因A和G频率分布具有差异性（P<0.05），妊娠高血压综合征组G等位基因频率高于对照组(χ²=4.21，P<0.05，OR =0.66，95%CI=0.45~0.99)。结论青海省瘦素基因G2548位点G/A基因多态性与其妊娠高血压的易感性有关。G等位基因可能为妊娠高血压综合征的易感基因，A等位基因可能为妊娠高血压综合征的保护基因。

Abstract

Objective To investigate the relationship between polymorphism of leptin gene promoter region G-2548 A and pregnancy-induced hypertension syndrome（PIH）in Han population of Qinghai Province. Methods A total of 130 PIH subjects and 135 normal pregnancy subjects were studied. The genotype of leptin was analyzed by polymerase chain reaction fragment length polymorphism (PCR-RFLP). The mutation was confirmed by sequencing. Results The frequencies of leptin GG, GA, and AA genotype in the PIH group were 53.1%, 40% and 6.9%, and in the control group were 60%, 34.1% and 5.9% respectively. There was difference in frequency distribution of leptin genotype between the PIH and control groups. The frequency of G gene in the PIH group was higher than the control group (χ²=4.21，P<0.05，OR=0.66，95%CI=0.45-0.98). Conclusion Leptin gene G-2548 A is significantly correlated with PIH in Han group of QingHai. G allele may be a susceptible gene to PIH (OR=1.361, 95%CI: 1.01-1.83) and A allele may be a protective gene to PIH (OR=0.901, 95%CI: 0.82-1.00).

导出引用

庄文婷赵得雄王香林李红荣李长兴李福鑫孔得霞李建华. 青海省妊娠高血压综合征患者瘦素基因启动子区G-2548 A单核苷酸的多态性[J]. 解剖学报. 2018, 49(4): 524-528 https://doi.org/10.16098/j.issn.0529-1356.2018.04.018

ZHUANG Wen-ting ZHAO De-xiong WANG Xiang-lin LI Hong-rong LI Chang-xing LI Fu-xin KONG De-xia LI Jian-hua. Polymorphism of leptin gene G-2548 A and pregnancy-induced hypertension syndrome in Han population of Qinghai Province[J]. Acta Anatomica Sinica. 2018, 49(4): 524-528 https://doi.org/10.16098/j.issn.0529-1356.2018.04.018

参考文献

［1］ Le J. Obstetrics and Gynecology［M］. 7th eds. Beijing: People’s Health Publishing House, 2008:92-101. （in Chinese）

乐杰. 妇产科学［M］.第7版.北京:人民卫生出版社,2008:92-101.

［2］ Kuang GF, Yang XJ, Zong LSh. Progress in the relationship between hypoxia-inducible factor-1 and pathological pregnancy［J］. Acta Academiae Medicine Qingdao University, 2013, 49(6):562-564. （in Chinese）

匡国芳, 杨小菊,宗丽莎. 低氧诱导因子-1与病理妊娠关系的研究进展［J］. 青岛大学医学院学报, 2013, 49(6): 562-564.

［3］ Cao ZY. Chinese Obstetrics and Gynecology［M］. 3th eds. Beijing: People’s Medical Publishing House, 2014:503-521.(in Chinese)

曹泽毅. 中华妇产科学［M］.第3版. 北京: 人民卫生出版社，2014:503-521

［4］ Muy-Rivera M, Ning Y, Frederic IO, et al. Leptin，soluble leptin receptor and leptin gene polymorphism in relation to preeclampsia risk［J］. Physiol Res, 2005, 54 (2):167-174.

［5］ Su ChF, Zhang YH. Leptin and cardiovascular diseases［J］. Shanxi Medical Journal, 2011, 40(4):368-370. (in Chinese)

苏春芳，张颜华. 瘦素与心血管疾病［J］.山西医药杂志, 2011, 40(4)：368-370.

［6］ Ibrahim HS, Omar E, Froemming GR, et al. Leptin increases blood pressure and markers of endothelial activation during pregnancy in rats［J］. Biomed Res Int, 2013, 2013:298401.

［7］ Farias DR, Franco-sena AB, Rebelo F, et al. Polymorphisms of leptin (G-2548A) and leptin receptor (Q223R and K109R) genes and blood pressure during pregnancy and the postpartum period: a cohort［J］. Am J Hypertens, 2017,30(2):1-11.

［8］ Gao JJ, Zhang WZh. Association between polymorphism of leptin gene G-2548A and susceptibility to asthma［J］. Chinese Journal of Immunology, 2009,29(3):240-246. (in Chinese)

高俊杰, 张维忠. 瘦素基因G-2548A 位点多态性与哮喘的相关性研究［J］. 中国免疫学杂志, 2009, 29(3): 240-246

［9］ Guo L, Zuo HJ. Advances in the study of the etiology of pregnancy-induced hypertension［J］. Experimental and Laboratory Medicine, 2014, 32(4):414-416. (in Chinese)

郭玲, 左慧君. 妊娠高血压疾病病因学的研究进展［J］. 实验与检验医学, 2014, 32(4): 414-416

［10］ Miehle K, Stepan H, Fasshauer M. Leptin, adiponectin and other adipocytes in gestational diabetes mellitus and preeclampsia［J］. Clin Endocrinol Oxf, 2012, (76):2-11.

［11］ Abelenda M, Ledesma A, Rial E, et al. Leptin administration to cold-acclimated rats reduces both food intake and brown adipose tissue thermogenesis［J］. J Ther Biol, 2003,28(6-7): 525-530.

［12］ Hauguel-de Mouzon S, Lepercq J, Catalano P. The known and unknown of leptin in pregnancy［J］. Am J Obstet Gynecol, 2006, 194 (6):1537-1545.

［13］ Kalindeis M, papanikolaou A, Kalinderi K, et al. Serum levels of leptin and IP-10 in preeclampsia compared to controls ［J］. Arch Gynecol Obestet, 2015, 292(2): 343-347.

［14］ Hall JE, Hildebrandt DA, Kuo J. Obesity hypertension: role of leptin and sympathetic nervous system ［J］. Am J Hypertens, 2001, 14 (6 Pt 2): 103-115.

［15］ Liu BG, Li H, Liu DQ. Study on clinical significance of serum leptin,cystatin C and human chorionic gonadotropin in patients with pregnancy-induced hypertension［J］. Journal of Clinical and Experimental Medicine, 2015,14(1):12-14. (in Chinese)

刘炳刚, 李红, 刘大庆. 血清瘦素、胱抑素C和人绒毛膜促性腺激素在妊娠高血压综合征检测中的意义［J］. 临床和实验医学杂志, 2015, 14(1): 1214.

［16］ Chiu CZ, Wang BW, Shyu KG. Molecular regulation of the expression of leptin by hypoxia in human coronary artery smooth muscle cells［J］. J Biomed Sci, 2015,22:5-19.

［17］ Klaffenbach D, Meiβner U, Raake M, et al. Upregulation of leptin receptor in placental cells by hypoxia ［J］. Regul Pept, 2011, 167(1):156-162.

［18］ Bodnar LM, Catov JM, Klebanoff MA, et al. Prepregnancy body mass index and the occurrence of severe hypertensive disorders of pregnancy［J］. Epidemiology, 2007, 18 (2):234-239.

［19］ Beltowski J. Leptin and atherosclerosis［J］. Atherosclerosis, 2006, 189(1):47-60.

［20］ Berg AH, Scherer PE. Adipose tissue, inflammation, and cardiovascular disease［J］. Circ Res, 2005, 96(9):939-949.

［21］ Taylor BD, Ness RB, Olsen J, et al. Serum leptin measured in early pregnancy is higher in women with preeclampsia compared with normotensive pregnant women. ［J］.Hypertension, 2015, 65(3): 594-599.

［22］ Song Y, Gao J, Qu Y, et al. Serum levels of leptin, adiponectin and resist in relation to clinical characteristics in normal pregnancy and preeclampsia［J］. Clin Chim Acta, 2016, 458: 133-137.

［23］ Boumaiza Ⅰ, Omezzine A, Rejeb J, et al. Relationship between leptin G2548A and leptin receptor Q223R gene polymorphisms and obesity and metabolic syndrome risk in Tunisian volunteers［J］. Genet Test Mol Biomarkers，2012，16（7）:726-733.

［24］ Hoffstedt J, Eriksson P, Mottaggui-Tabar S, et al. A polymorphism in the leptin promoter region(-2548G/A) influences gene expression and tissue secretion of leptin［J］. Horm MetabRes, 2002,34(7):355-359.

［25］ Ribeiro R, Vasconcelos A, Costa S, et al. Overexpressing leptin genetic polymorphism (2548G/A) is associated with susceptibility to prostate cancer and risk of advanced disease［J］. Prostate, 2004, 59(3): 268-274.

［26］ Xu HX, Zhang YH, Gao L, et al. Association of leptin gene promoter G-2548A polymorphism and hypertension in young people［J］. Journal of Ningxia Medical University, 2010, 32(5):603-605. (in Chinese)

许红霞，张毓洪，高岭, 等. 瘦素基因启动子区G-2548A多态性与青年高血压的相关性研究［J］. 宁夏医科大学学报, 2010,32(5):603-605.