目的 探讨脑脂结合蛋白（BLBP）对体外培养的星形胶质细胞增殖能力的影响。 方法 在体外培养大鼠星形胶质细胞的基础上，应用小干扰RNA（siRNA）沉默细胞内源性BLBP的表达后，Real-time PCR和Western blotting分别检测BLBP及c-Myc基因和蛋白的表达变化；随后采用细胞计数试剂盒8（CCK-8）试剂检测细胞活力，Ki67免疫荧光标记增殖状态的细胞，并用流式细胞术检测细胞周期的变化。 结果 应用siRNA沉默星形胶质细胞内源性BLBP的表达后，BLBP基因和蛋白的表达量明显下降，原癌基因c-Myc的表达水平也显著下调；BLBP基因沉默能够抑制细胞的活力并减少Ki67阳性细胞的数量；流式细胞术检测后发现，BLBP表达不足能够减少S期细胞的比例。 结论 BLBP基因沉默能够抑制体外培养的星形胶质细胞的增殖能力，BLBP在星形胶质细胞的增殖过程中发挥调控作用。

Objective To explore the function of brain-lipid binding protein (BLBP) in cultured rat astrocytes in vitro. Methods After culturing rat astrocytes, BLBP small interference RNA (siRNA) was used to silence the expression of endogenous BLBP. Real-time PCR and Western blotting were applied to detect the level of BLBP and c-Myc; the cells viability was detected by using CCK-8 regent, the proliferated cells were labeled by Ki67, and the cells cycle was investigated by flow cytometry. Results The expressions of BLBP and c-Myc were significantly decreased after treatment of BLBP siRNA. BLBP gene silencing suppressed the cells viability and decreased the proportion of Ki67-positive cells. The result of flow cytometry showed that the insufficient expression of BLBP reduced the cells number of S phase. Conclusion The gene silencing of BLBP inhibits the cells proliferation of astrocytes in vitro. BLBP may regulate the proliferation process of astrocytes.