三类生物因子对涡虫生殖和再生的影响

董自梅 董艳萍 陈广文* 刘德增

doi:10.16098/j.issn.0529-1356.2017.06.017

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解剖学报 ›› 2017, Vol. 48 ›› Issue (6) : 732-739. DOI: 10.16098/j.issn.0529-1356.2017.06.017

组织学胚胎学发育生物学

三类生物因子对涡虫生殖和再生的影响

董自梅董艳萍陈广文* 刘德增

作者信息 +

Influence of three kinds of biological factors on the reproduction and regeneration in planarians

DONG Zi-mei DONG Yan-ping CHEN Guang-wen* LIU De-zeng

Author information +

文章历史 +

摘要

目的以国内广布种日本三角涡虫（Dugesia japonica）为受试动物，探讨三类生物因子对涡虫生殖和再生的影响。方法选择与生殖相关的激素（雌二醇、甲基睾丸素），生物胺（5-羟色胺、多巴胺）和D-氨基酸（D-色氨酸、D-精氨酸、D-苯丙氨酸）对日本三角涡虫进行急性毒性实验，用SPSS 17. 0统计学软件计算出适宜的实验浓度，对整体及再生涡虫进行培养，观察并记录实验现象。结果雌二醇、5-羟色胺、D-色氨酸和D-苯丙氨酸处理组分别引起20%、40%、20%和20%涡虫卵巢及交配器官的发育；而多巴胺则引起40%～50%涡虫生殖腺退化。另外，5-羟色胺处理组涡虫头部再生速度加快，而多巴胺处理组则头部再生速度减慢。结论雌二醇、5-羟色胺、D-色氨酸和D-苯丙氨酸能够诱导涡虫生殖腺的发育，并且5-羟色胺促进涡虫头部再生；多巴胺则引起涡虫生殖腺退化并对涡虫再生有抑制作用。

Abstract

Objective To investigate the effects of the three reproductive-relative factors on gonads and regeneration in planarians. Methods In this study, the planarians Dugesia japonica were cultured with three reproductive-relative factors: the hormones ［estradiol (E2), methyltestosteronum(MT)］, biogenic amine ［5-hydroxy-tryptamine (5-HT),dopamine (DA)］ and D-amino acids (D-tryptophan, D-arginine, D-phenylalanine). The cultured concentration was calculated by SPSS17.0 after the acute toxicity experiment, and the experimental phenomena were recorded. Results E2, 5-HT, D-tryptophan and D-phenylalanine improved the gonads development, which proportions were 20%, 40%, 20%, and 20% respectively, but DA made the gonads atrophy in 40% or 50% planarians. Besides, 5-HT increased speed of regeneration, while DA inhibited it during planarians regeneration. Conclusion E2, 5-HT, D-tryptophan and D-phenylalanine improved the gonads development, and 5-HT increased speed of regeneration; DA inhibited the gonads and regeneration.

导出引用

董自梅董艳萍陈广文刘德增. 三类生物因子对涡虫生殖和再生的影响[J]. 解剖学报. 2017, 48(6): 732-739 https://doi.org/10.16098/j.issn.0529-1356.2017.06.017

DONG Zi-mei DONG Yan-ping CHEN Guang-wen LIU De-zeng. Influence of three kinds of biological factors on the reproduction and regeneration in planarians[J]. Acta Anatomica Sinica. 2017, 48(6): 732-739 https://doi.org/10.16098/j.issn.0529-1356.2017.06.017

参考文献

［1］Ma KX, Chen GW, Lou H, et al. Effects of prolonged starvation on aminotransferases, phosphatases, proteinases and proliferating cell nuclear antigens in planarians［J］. Acta Anatomica Sinica, 2010,41(3):383-386. (in Chinese)

马克学, 陈广文, 娄昊, 等.持久饥饿对涡虫转氨酶、磷酸酶、蛋白水解酶和增殖细胞核抗原的影响［J］.解剖学报, 2010, 41(3):383-386.

［2］Sikes JM, Newmark PA. Restoration of anterior regeneration in a planarian with limited regenerative ability［J］. Nature, 2013, 500(7460): 77-80.

［3］Petersen CP, Reddien PW. Polarized activation of notum at wounds inhibits Wnt signaling to promote planarian head regeneration［J］. Science, 2011, 332(6031): 852-855.

［4］Kobayashi K, Koyanagi R, Matsumoto M, et al. Switching from asexual to sexual reproduction in the planarian Dugesia ryukyuensis: bioassay system and basic description of sexualizing process［J］. Zoolog Sci, 1999, 16(2): 291-298.

［5］Shi CY, Dong ZM, Zhang HC, et al. Cloning and characterization of DjPRPS gene in freshwater planarian Dugesia japonica［J］. Turk J Biochem, 2015, 40(1):58-65.

［6］Wagner DE, Wang IE, Reddien PW. Clonogenic neoblasts are pluripotent adult stem cells that underlie planarian regeneration［J］. Science, 2011, 332(6031): 811-816.

［7］Chong T, Stary JM, Wang Y, et al. Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea［J］. BMC Dev Biol,2011, 11(1):69-81.

［8］Liu ChL. Research progress of reproduction and regeneration in freshwater planarian［J］. Journal of Liu’an Teachers College, 2000,16(2): 31-33. (in Chinese)

刘昌利.淡水涡虫繁殖及再生的研究进展［J］.六安师专学报, 2000,16(2): 31-33.

［9］Kenk R. Induction of sexuality in the asexual form of Dugesia tigrina (Girard)［J］. J Exp Zool, 1941,87(1):55-69.

［10］Okugawa KI. An experimental study of sexual induction in the asexual form of Japanese fresh-water Planarian, Dugesia gonocephala(Dugés)［J］. Bull Kyoto Gakugei Univ, 1957, 11(1):8-27.

［11］Kobayashi K, Hoshi M. Sex-inducing effect of a hydrophilic fraction on reproductive switching in the planarian Dugesia ryukyuensis (Seriata, Tricladida)［J］. Front Zool, 2011, 8(1):23-34.

［12］Fukushima M, Funabiki I, Hashizume T, et al. Detection and changes in levels of testosterone during spermatogenesis in the freshwater planarian Bdellocephala brunnea［J］. Zoolog Sci, 2008,25(7):760-765.

［13］Miyashita H, Nakagawa H, Kobayashi K, et al. Effects of 17β-estradiol and bisphenol A on the formation of reproductive organs in planarian［J］. Biol Bull, 2011, 220(1):47-56.

［14］Maezawa T, Tanaka H, Nakagawa H, et al. Planarian D-amino acid oxidase is involved in ovarian development during sexual induction［J］. Mech Dev, 2014, 132(1):69-78.

［15］Liang W, Chen Zh. Research progress of the main neurotransmitter distribution and function in poultry ovary［J］. China Poultry, 2016, (20):41-46. (in Chinese)

梁薇,陈忠. 家禽卵巢主要神经递质分布及其功能的研究进展［J］. 中国家禽, 2016,(20):41-46.

［16］Li H, Ding C, Ding ZL, et al. 17β-Oestradiol promotes differentiation of human embryonic stem cells into dopamine neurons via cross-talk between insulin-like growth factors-1 and oestrogen receptor β［J］. J Cell Mol Med, 2017, 21(8):1605-1618.

［17］Mylenko M, Boland S, Penkov S, et al. NAD+ is a food component that promotes exit from dauer diapause in Caenorhabditis elegans［J］. PLoS One, 2016, 11(12):e0167208.

［18］Tinikul Y, Mercier AJ, Soonklang N, et al. Changes in the levels of seretonin and dopamine in the central nervous system and ovary，and their possible roles in the ovarian development in the giant freshwater prawn，Macrobrachium rosenbergii［J］. Gen Comp Endocrinol, 2008, 158(3):250-258.

［19］Collins JJ III, Hou X, Romanova EV, et al. Genome-wide analysis reveal a role for peptide hormones in planarian germline development［J］. PLoS Biol, 2010,8(10):1-21.

［20］Dong ZM, Li XY, Chen J, et al. Review on the genes involved in eyes morphogenesis in freshwater planarian［J］. Acta Anatomica Sinica, 2015, 46(3):422-427. (in Chinese)

董自梅,李小艳,陈静，等.淡水涡虫眼点形态发生相关基因研究进展［J］.解剖学报, 2015,46(3):422-427.

［21］Nishimura O, Hosoda K, Kawaguchi E, et al. Unusually large number of mutations in asexually reproducing clonal planarian Dugesia japonica［J］. PLoS One, 2015,10(11):e0143525.

［22］Fukuhori N, Kitano M, Kitamura H. Toxic effects of bisphenol A on sexual and asexual reproduction in Hydra oligactis［J］. Arch Environ Contam Toxicol, 2005,48(4): 495-500.

［23］Zou Q. Study on the disspation of 17α-methyltestosterone and Hplc determination of its residues in Nile Tilapia［D］. Guangdong Ocean University, 2010. (in Chinese)

邹琴.甲基睾丸酮在罗非鱼体内的消解规律及检测方法的研究［D］.广东海洋大学, 2010.

［24］Zhuo XL, Zou JX, Cui K, et al. The influence of exogenous methyltestosterone on the gonad developmental process of female and intersexual monopterus albus［J］. Acta Hydrobiologica Sinica, 2008, 32(6):861-865. (in Chinese)

卓孝磊,邹记兴,崔科,等.外源性甲基睾丸酮对雌性和间性黄鳝性腺发育的影响［J］.水生生物学报，2008, 32(6):861-865.

［25］Li ShQ, Yang B, Xu ChT. The research status for neurotransmitter 5-hydroxytryptamine［J］. Clinical Medical Engineering, 2010, 17(5):145-147. (in Chinese)

李陕区,杨博,许昌泰. 神经递质5-羟色胺研究现状［J］.临床医学工程, 2010,17(5):145-147.

［26］Ding JH, Fu ChG, Zhao RH. Current status of hydroxytryptamine in functional gastric intestinal diseases［J］. World Chinese Journal of Digestology, 2005, 13(20):2405-2408. (in Chinese)

丁健华，傅传刚，赵荣华.五羟色胺在胃肠道功能性疾病中的研究现状［J］.世界华人消化杂志, 2005, 13(20)：2405-2408.

［27］Yokoyama T, Saino T, Nakamuta N, et al. Topographic distribution of serotonin-immunoreactive urethral endocrine cells and their relationship with calcitonin gene-related peptide-immunoreactive nerves in male rats［J］. Acta Histochem, 2017, 119(1):78-83.

［28］Fang Q, Fang YQ, Wong YZh, et al. Distribution of neurotransmitters and regulatory peptide immunoreactive substances in ovary of Crassostrea gigas［J］. Journal of Oceanography in Taiwan Strait, 2003, 22(3):299-302. (in Chinese)

方琦,方永强,翁幼竹,等. 神经递质和调节肽免疫活性物质在长牡蛎卵巢中的分布［J］.台湾海峡, 2003, 22(3):299-302.

［29］Ye HH, Li ShQ, Li QF, et al. The effects of biogenic amines on ovarian development in the mud crab, scylla serrata［J］. Oceanologia et Limnologia Sinica, 2003, 34(3):329-333. (in Chinese)

叶海辉,李少菁,李祺福,等. 生物胺对雌性锯缘青蟹生殖神经内分泌的调控作用［J］.海洋与湖沼, 2003, 34(3):329-333.

［30］Li PSh. Structure and function study of digestive tract in Dugesia japonica［D］. Henan Normal University, 2011. (in Chinese)

李鹏声.三角涡虫（Dugesia）消化道组织结构和功能的研究［D］.河南师范大学, 2011.

［31］Ninomiya Y, Hayakawa Y. Insect cytokine, growth-blocking peptide, is a primary regulator of melanin-synthesis enzymes in armyworm larval cuticle［J］. FEBS J, 2007, 274(7):1768-1777.

［32］Watanabe T, Sadamoto H, Aonuma H. Identication and expression analysis of the genes involved in serotonin biosynthesis and transduction in the eld cricket Gryllus bimaculatus［J］. Insect Mol Biol, 2011,20(5):619-635.

［33］Watanabe T, Sadamoto H, Aonuma H. Molecular basis of the dopaminergic system in the cricket Gryllus bimaculatus［J］. Invert Neurosci, 2013, 13(2):107-123.

［34] Pastor D, Piulachs MD, Cassier P, et al. Etude in vivo et in vitro de l‘action de la dopamine sur la croissance des ovocytes et la production d’hormone juve’nile chez Blattella germanica (L.) (Dictyoptera; Blattellidae) ［J］.C R Acad Sci III, 1991, 313(1):207-212.

［35］Neckameyer WS. Multiple roles for dopamine in Drosophila development［J］. Dev Biol, 1996, 176(2):209-219.

［36］Vergoz V, Lim J, Oldroyd BP. Biogenic amine receptor gene expression in the ovarian tissue of the honey bee Apis mellifera［J］. Insect Mol Biol, 2012, 21(1):21-29.

［37］Nishimura K, Kitamura Y, Lnoue T, et al, Reconstruction of dopaminergic neural network and locomotion function in planarian regenerates［J］. Dev Neurobiol, 2007, 67(8):1059-1078.

［38］Khoronenkova SV, Tishkov VI. D-amino acid oxidase: physiological role and applications［J］. Biochem Biokhimiia, 2008, 73(13):1511-1518.

［39］Panatier A, Theodosis DT, Mothet JP, et al. Glia-derived D-serine controls NMDA receptor activity and synaptic memory［J］. Cell, 2006, 125(4):775-784.

［40］Gustafson EC, Stevens ER, Wolosker H, et al. Endogenous d-serine contributes to NMDA receptor-mediated light-evoked responses in the vertebrate retina［J］. J Neurophysiol, 2007, 98(1):122-130.

［41］Henneberger C, Papouin T, Oliet SH, et al. Long-term potentiation depends on release of D-serine from astrocytes［J］. Nature, 2010, 463(7278):232-236.

［42］Ota N, Shi T, Sweedler JV. D-Aspartate acts as a signaling molecule in nervous and neuroendocrine systems［J］. Amino Acids, 2012, 43(5):1873-1886.

［43］Aniello G, Ronsini S, Guida F, et al. Occurrence of d-aspartic acid in human seminal plasma and spermatozoa: possible role in reproduction［J］. Fertil Steril, 2005, 84(5):1444-1449

［44］Yamamoto A, Tanaka H, Ishida T, et al. D-Aspartate oxidase localization in pituitary and pineal glands of the female pig［J］. J Neuroendocrinol, 2010,22(11):1165-1172.

［45］Saitoh Y, Katane M, Kawata T, et al. Spatiotemporal localization of D-amino acid oxidase and D-aspartate oxidases during development in Caenorhabditis elegans［J］. Mol Cell Biol, 2012, 32(10):1967-1983.