鳕鱼皮寡肽对人胃癌细胞SGC-7901增殖凋亡的影响及机制

吴丽萍 续力云 胡晓斐 张国强*

doi:10.16098/j.issn.0529-1356.2017.06.011

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解剖学报 ›› 2017, Vol. 48 ›› Issue (6) : 698-703. DOI: 10.16098/j.issn.0529-1356.2017.06.011

肿瘤生物学

鳕鱼皮寡肽对人胃癌细胞SGC-7901增殖凋亡的影响及机制

吴丽萍¹ 续力云² 胡晓斐¹张国强^1*

作者信息 +

Effect of cod skin oligopeptide on the proliferation and apoptosis in human gastric cancer cells line SGC-7901 and its mechanism

WU Li-ping¹ XU Li-yun²HU Xiao-fei¹ZHANG Guo-qiang ^1*

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摘要

目的探讨鳕鱼皮寡肽（CSO）对人胃癌细胞（SGC-7901）的增殖影响。方法用不同浓度CSO处理体外培养的SGC-7901细胞后，荧光显微镜下观察细胞4’6-二脒基-2-苯基吲哚（DAPI）染色后细胞形态变化，应用CCK-8 实验检测细胞活力，免疫印迹法和流式细胞术检测细胞凋亡及细胞周期。结果 CSO对SGC-7901细胞增殖有明显的抑制作用，且并呈剂量、时间效应关系（P<0.05）。作用于SGC-7901胃癌细胞24 h、48 h和72 h的IC₅₀分别是156.90 g/L、102.10 g/L、73.13 g/L。DAPI染色后发现，SGC-7901细胞随着CSO浓度增加可见大量的细胞核碎裂，荧光强烈。24h细胞凋亡率检测显示，细胞随着浓度的增加凋亡率呈上升趋势，表明CSO对SGC-7901呈浓度效应关系。细胞周期观察发现，SGC-7901细胞G₁期细胞数随着CSO浓度的增加而递增，而S/G₂期细胞随着浓度的细胞递降。Caspase-3、Caspase-9随着CSO浓度的逐渐增高表达上调，Bcl-2表达下调。结论鳕鱼皮寡肽能抑制人胃癌细胞（SGC-7901）增殖，诱导凋亡，抑癌机制可能与Caspase-3、Caspase-9上调和Bcl-2下调相关。

Abstract

Objective To explore the effect of cod skin oligopeptide(CSO) on the proliferation in human gastric carcinoma SGC-7901 cells. Methods After treatment of SGC-7901 cells with different concentrations of CSO, the cells were observed under the microscope and the viability of cells was detected by CCK-8 assay. Cell apoptosis and cell cycle were determined by Western blotting and flow cytometry. Results CSO significantly suppressed the proliferation of SGC-7901 cells in a time-and dose-dependent manner（P<0.05）. The cultured SGC-7901 cells were treated by CSO for 24 hours and 48 hours and 72 hours with the IC50of 156.90 g/L and 102.10 g/L and 73.13 g/L.A large number of nuclear fragmentation and apoptotic bodies were observed under fluorescence microscope after treatment by DAPI. After treatment with CSO for 24 hours, SGC-7901 cells showed increased at G₁ stage and cell cycle arrested at S/G₂ stage, and the cell apoptosis rate increased with the CSO. With the increase of CSO concentration, the expression of Caspase-3 and Caspase-9 was up-regulated, and the expression of Bcl-2 was down-regulated. Conclusion CSO can promote the apoptosis and inhibit the proliferation of SGC-7901 cells. Anti-cancer mechanism may be related with Caspase-3 and Caspase-9 up-regulating,and Bcl-2 was down-regulating.

导出引用

吴丽萍续力云胡晓斐张国强. 鳕鱼皮寡肽对人胃癌细胞SGC-7901增殖凋亡的影响及机制[J]. 解剖学报. 2017, 48(6): 698-703 https://doi.org/10.16098/j.issn.0529-1356.2017.06.011

WU Li-ping XU Li-yun HU Xiao-fei ZHANG Guo-qiang. Effect of cod skin oligopeptide on the proliferation and apoptosis in human gastric cancer cells line SGC-7901 and its mechanism[J]. Acta Anatomica Sinica. 2017, 48(6): 698-703 https://doi.org/10.16098/j.issn.0529-1356.2017.06.011

参考文献

［1］Costa M, Garcia M, Costa-Rodrigues J,et al.Exploring bioactive properties of marine cyanobacteria isolated from the portuguese coast: high potential as a source of anticancer compounds［J］.Marine Drugs,2014,12(1): P 98-114.
［2］Talero E, García-Mauri?o S, ávila-Román J,et al.Bioactive Compounds Isolated from Microalgae in Chronic Inflammation and Cancer［J］.Mar Drugs, 2015, 13(10), 6152-6209.
［3］Huang HN, Rajanbabu V, Pan CY, et al.Enhanced control of bladder-associated tumors using shrimp anti-lipopolysaccharide factor (SALF) antimicrobial peptide as a cancer vaccine adjuvant in mice［J］.Mar Drugs, 2015,13(5), 3241-3258.
［4］Hartgrink HH, Jansen EP, van Grieken NC, et al. Gastric cancer［J］. Lancet, 2009, 374(9688): 477-490.
［5］Wu LP, Hu XF, Wan HP, et al.Cod skin peptide reduces chemotherapy-induced toxicity in gastric cancer patients［J］. Asia Pac J Clin Nutr,2016,25(4):760-766. 
［6］Du Y, Ji X.Bcl-2 down-regulation by small interfering RNA induces Beclin1-dependent autophagy in human SGC-7901 cells［J］. Cell Biol Int, 2014, 38(10):1155-1162. 
［7］Shi SL, Chen LY,Liu YJ,et al.Expression and localization changes of nucleophosmin during apoptosis of human esophageal cancer EC9706 cells induced by curcumin［J］.Acta Anatomica Sinica,2014,45(2):221-227. (in Chinese)
石松林,陈兰英,刘用金,等.姜黄素诱导人食管癌EC9706细胞凋亡过程中核磷蛋白的表达与定位变化［J］.解剖学报,2014,45(2):221-227.
［8］Wang Y, Zhao Sh, Su YP,et al.Sodium selenite induces apoptosis of human gastric cancer SGC-7901 cells through mitochondrial-dependent pathway［J］.Acta Anatomica Sinica,2016, 47 (3):353-358. (in Chinese)
王艳,赵上,苏衍萍,等.亚硒酸钠通过线粒体途径诱导人胃癌SGC-7901细胞凋亡的机制［J］.解剖学报, 2016, 47 (3):353-358.
［9］Sun HJ, Meng LY, Shen Y,et al.S-benzyl-cysteine-mediated cell cycle arrest and apoptosis involving activation of mitochondrial-dependent caspase cascade through the p53 pathway in human gastric cancer SGC-7901 cells［J］. Asian Pac J Cancer Prev, 2013,14(11):6379-6384.
［10］Zhang XJ,Mei WL, Tan GH,et al.Strophalloside induces apoptosis of SGC-7901 cells through the mitochondrion-dependent Caspase-3 pathway［J］. Molecules, 2015, 31,20(4):5714-5728. 
［11］Sui CG, Meng FD, Li Y,et al.Antiproliferative activity of rosamultic acid is associated with induction of apoptosis, cell cycle arrest, inhibition of cell migration and caspase activation in human gastric cancer (SGC-7901) cells［J］. Phytomedicine, 2015, 15,22(9):796-806.