目的 探讨Raptor是否可以通过Wnt3a/β-catenin信号通路促进乳腺癌细胞的侵袭与转移。 方法 采用免疫印迹法（Western blotting）检测乳腺癌细胞MCF-7和MDA-MB-231(MDA231)中Raptor蛋白的表达情况；采用小RNA（RNAi）干扰技术将质粒转染MDA231，将过表达质粒转染MCF-7；趋化运动实验检测乳腺癌细胞的运动能力；Transwell侵袭实验检测乳腺癌细胞的侵袭能力；Western blotting检测上皮细胞间充质转化（EMT）标志物的表达情况；核质分离实验检测β-联蛋白（β-catenin）的转核情况。 结果 Western blotting法检测结果显示，Raptor在低转移细胞系MCF-7中低表达，而在高转移细胞系MDA231中高表达。转染后，SiRaptor/MDA231细胞组中Raptor蛋白的表达明显下降，MCF-7/Raptor细胞组中Raptor蛋白的表达明显升高，表明转染成功。用Wnt3a刺激处理后，趋化运动和Transwell侵袭实验结果显示，SiRaptor/MDA231细胞组运动和侵袭能力明显降低，MCF-7/Raptor细胞组运动和侵袭能力明显增强。Western blotting法检测结果显示，用Wnt3a和抑制剂Dkk1不同处理后，SiRaptor/MDA231细胞组中上皮-钙黏蛋白（E-cadherin）上调，波形蛋白（vimentin）下调，细胞核中β-0catenin的表达降低，而MCF-7/Raptor细胞组中E-cadherin蛋白下调，vimentin蛋白上调，β-catenin蛋白的表达升高。 结论 Raptor能通过Wnt3a/β-catenin信号通路促进EMT的发生，进而促进乳腺癌细胞的侵袭与转移。

Objective To explore whether the Raptor can promote invasion and metastasis of breast cancer cells through Wnt3a/-catenin signaling pathway. Methods Using Western blotting to detect the protein expression of Raptor in breast cancer cells MDA-MB-231(MDA231) and MCF-7; Using RNAi technology to transfect small RNA interference plasmid into MDA231 breast cancer cells transiently. Overexpression of plasmid was transfected into MCF-7 breast cancer cells. Transwell and chemotaxis assay were used to detect the invasion and chemotaxis ability of breast cancer cells. Western blotting detected the expression of epithelial-mesenchymal transition(EMT) markers; Karyoplasm separation experiment tested the expression of β-catenin in nuclei. Results Western blotting result showed that the expression of Raptor was low in MCF-7, and high in MDA231. The protein expression of Raptor in SiRaptor/MDA231 was decreased obviously, and the protein expression of Raptor in MCF-7/Raptor was obviously higher. Transwell invasion and chemotaxis assay result showed that the invasion and chemotaxis ability of SiRaptor/MDA231 was decreased with the stimulation of Wnt3a, however, its capability of invasion and chemotaxis of MCF-7/Raptor was significantly enhanced. According to the result of Western blotting method with unused Wnt3a or at the same time stimulated with Wnt3a and Dkk1, the expression of E-cadherin was higher, and the expressions of vimentin and β-catenin were reduced in SiRaptor/MDA231, while in the MCF-7/Raptor, the expression of E-cadherin was reduced, and the expressions of vimentin and β-catenin were increased. Conclusion Through Wnt3a/β-catenin signaling pathway Raptor promotes the occurrence of EMT, thus to promote the invasion and metastasis of breast cancer cells.