脑内移植RMNE6细胞治疗缺血性脑损伤的效果

武赟* 翟晓艳 杨李旺 徐群渊

doi:10.16098/j.issn.0529-1356.2017.06.001

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解剖学报 ›› 2017, Vol. 48 ›› Issue (6) : 635-641. DOI: 10.16098/j.issn.0529-1356.2017.06.001

神经生物学

脑内移植RMNE6细胞治疗缺血性脑损伤的效果

武赟^1,2*翟晓艳¹杨李旺¹徐群渊²

作者信息 +

Effects of intracerebral transplantation of RMNE6 on the ischemic injury of the brain

WU Yun ^1,2* ZHAI Xiao-yan¹ YANG Li-wang¹ XU Qun-yuan²

Author information +

文章历史 +

摘要

目的为细胞替代治疗缺血性脑损伤选择合适的干细胞。方法将RMNE6细胞植入正常脑和缺血模型大鼠脑(8只)内，通过免疫荧光染色、行为学实验、影像学等方法观察其在脑内的状况，与安慰剂组（8只）及成纤维细胞移植组（8只）比较，观察其对模型动物梗塞体积的影响和模型动物行为学的改善。结果细胞移植后，RMNE6细胞在正常脑和缺血脑内可以存活，但移植有RMNE6细胞的缺血脑内出现肿瘤。细胞移植与否对梗死体积没有影响。细胞移植后，模型动物的行为学也未见明显改善。结论从安全性的角度考虑，RMNE6细胞不适合通过脑内移植治疗缺血性脑损伤，RMNE6细胞还需要改造。

Abstract

Objective To select the suitable stem cells for cell replacement in stroke therapy. Methods The RMNE6 cells were transplanted into the normal brain and injured brain of the stroke model of rats (n=8). The fates of the RMNE6 cells in the normal brain and the injured brain, the effects on the ischemic volume and the improvements on the behavior of the rats were observed by immunofluorescence staining, behaviour testing and magnetic resonance imaging, compared with the placebo group (n=8) and the fibroblast group (n=8). Results After transplantation, the RMNE6 cells were able to survive in the normal and ischemic brain area. There was tumourigenesis in the ischemic brain transplanted with the RMNE6 cells. The cell transplantation had no effects on the size of the ischemic volume. The behavior of the model animals had no significiant improvement. Conclusion As far as the safety is concerned, the RMNE6 cells are not suitable for cell replacement in the stroke therapy and still need to be modified.

导出引用

武赟翟晓艳杨李旺徐群渊. 脑内移植RMNE6细胞治疗缺血性脑损伤的效果[J]. 解剖学报. 2017, 48(6): 635-641 https://doi.org/10.16098/j.issn.0529-1356.2017.06.001

WU Yun ZHAI Xiao-yan YANG Li-wang XU Qun-yuan. Effects of intracerebral transplantation of RMNE6 on the ischemic injury of the brain[J]. Acta Anatomica Sinica. 2017, 48(6): 635-641 https://doi.org/10.16098/j.issn.0529-1356.2017.06.001

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