维生素D3对自然流产模型小鼠胚胎丢失率及外周血免疫细胞的影响

徐慧* 王玉兰 宋晓晖 黄娟

doi:10.16098/j.issn.0529-1356.2017.05.017

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解剖学报 ›› 2017, Vol. 48 ›› Issue (5) : 595-599. DOI: 10.16098/j.issn.0529-1356.2017.05.017

组织学胚胎学发育生物学

维生素D3对自然流产模型小鼠胚胎丢失率及外周血免疫细胞的影响

徐慧^1* 王玉兰² 宋晓晖¹ 黄娟³

作者信息 +

Influence of vitamin D3 on embryo loss rate and peripheral immune cells in mouse model of spontaneous abortion

XU Hui^1* WANG Yu-lan² SONG Xiao-hui¹ HUANG Juan³

Author information +

文章历史 +

摘要

目的探讨维生素D3（VitD₃）对自然流产模型小鼠胚胎丢失率及外周血免疫细胞的影响。方法采用CBA/J×BALB/c建立正常妊娠小鼠模型，采用CBA/J×DBA/2建立自然流产小鼠模型，实验动物分5组：正常组、自然流产组、VitD3低剂量组、中剂量组和高剂量组，每组15只。VitD₃各组自妊娠第1天开始腹腔注射VitD₃溶液，单次VitD₃给药剂量分别为1μg、4μg、16μg。记录和观察各组小鼠胚胎丢失情况，检测各组血清调节性T细胞（Treg）细胞因子：白细胞介素-10（IL-10），辅助性T细胞17（Th17）细胞因子：白细胞介素-17A（IL-17A）以及各组外周血Treg、Th17。结果与正常组相比，自然流产组胚胎丢失率显著升高（χ2=16.045，P＜0.05）；与自然流产组比较，VitD₃低剂量组、中剂量组和高剂量组胚胎丢失率显著降低（χ2=5.881、13.704、15.663，P＜0.05）。与正常组相比，自然流产组Treg、IL-10、Treg/Th17、IL-10/IL-17A显著降低，Th17、IL-17A显著升高，差异均有统计学意义（P＜0.05）；与自然流产组比较，VitD₃低剂量组、中剂量组和高剂量组Treg、IL-10、Treg/Th17、IL-10/IL-17A显著升高，Th17、IL-17A显著降低，差异均有统计学意义（P＜0.05）。结论 VitD₃能够降低自然流产模型小鼠胚胎丢失率，可能与调节Treg/Th17平衡有关。

Abstract

Objective To explore the influence of vitamin D3 (VitD₃) on embryo loss rate and peripheral immune cells in a mouse model of spontaneous abortion. Methods CBA/J×BALB/c was adopted to establish the mouse model of normal pregnancy, while CBA/J×DBA/2 was adopted to establish the mouse model of spontaneous abortion. There were totally 5 groups: normal group, spontaneous abortion group, VitD₃ low dose group, middle dose group and high dose groups. The VitD₃ groups were treated by VitD₃ solution diluted in saline with intraperitoneal injection of 1μg, 4μg, 16μg, respectively, 15 cases per group. The embryo loss situation in each group was recorded and observed. The regulatory T cells (Treg) factor, interleukin-10 (IL-10) and helper T cells 17(Th17) cytokines, interleukin-17A (IL-17A), as well as the Treg and Th17 cell were detected. Results Compared with the normal group, a significant rise of embryo loss rate presented in spontaneous abortion group (χ2=16.045, P<0.05); Compared with spontaneous abortion group, the embryo loss rate in VitD₃low dose group, middle dose group and high dose group reduced significantly (χ2=5.881, 13.704, 15.663, P<0.05). Compared with the normal group, the Treg, IL-10, Treg/Th17, IL-10/IL-17A in spontaneous abortion group reduced significantly; Th17 and IL-17A increased significantly, and the differences were statistically significant (P<0.05). Compared with spontaneous abortion group, the Treg, IL-10, Treg/Th17, IL-10/IL-17A in VitD₃low dose group, middle dose group and high dose group increased significantly, while the Th17 and IL-17A decreased significantly, and they all had significantly difference based on statistical analysis (P<0.05).Conclusion VitD₃can reduce the embryo loss rate in the mouse model of spontaneous abortion, which may be related to adjusting the balance of Th17/Treg.

导出引用

徐慧王玉兰宋晓晖黄娟. 维生素D3对自然流产模型小鼠胚胎丢失率及外周血免疫细胞的影响[J]. 解剖学报. 2017, 48(5): 595-599 https://doi.org/10.16098/j.issn.0529-1356.2017.05.017

XU Hui WANG Yu-lan SONG Xiao-hui HUANG Juan. Influence of vitamin D3 on embryo loss rate and peripheral immune cells in mouse model of spontaneous abortion[J]. Acta Anatomica Sinica. 2017, 48(5): 595-599 https://doi.org/10.16098/j.issn.0529-1356.2017.05.017

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