靶向沉默CD105和Ki67的表达对卵巢癌OVCAR3细胞生物学行为的影响

郭海荣 贺帅 王晓燕 刘萍*

doi:10.16098/j.issn.0529-1356.2017.04.011

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解剖学报 ›› 2017, Vol. 48 ›› Issue (4) : 434-439. DOI: 10.16098/j.issn.0529-1356.2017.04.011

肿瘤生物学

靶向沉默CD105和Ki67的表达对卵巢癌OVCAR3细胞生物学行为的影响

郭海荣¹ 贺帅²王晓燕¹ 刘萍^1*

作者信息 +

Impacts of silencing CD105 and Ki67 gene expression on the biological behavior of ovarian cancer OVCAR3 cells

GUO Hai-rong¹ HE Shuai²WANG Xiao-yan¹ LIU Ping^1*

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文章历史 +

摘要

目的探讨沉默CD105和Ki67基因表达对人卵巢上皮癌OVCAR3细胞系生物学行为的影响。方法 CD105-siRNA、Ki67-siRNA、CD105-siRNA+Ki67-siRNA、阴性对照组分别转染卵巢癌OVCAR3细胞，用MTT法、划痕实验、Transwell小室及流式细胞术检测CD105、Ki67单基因沉默及联合基因沉默对人卵巢癌细胞增殖、迁移、侵袭及凋亡的影响。结果与各自的空白对照组及空脂质体对照组相比，单基因CD105-siRNA组、单基因Ki67-siRNA组和双基因联合干预组基因沉默后人卵巢癌OVCAR3细胞的增殖能力、迁移能力及侵袭能力均明显下调，其中联合干预组下降最为明显，癌细胞凋亡率明显增加，其中联合干预组增加最为明显，差异存在统计学意义（P<0.01,P<0.05）。结论 CD105-siRNA和Ki67-siRNA表达载体均可抑制人卵巢癌OVCAR3细胞的增殖，并降低其细胞迁移和侵袭能力，诱导其肿瘤细胞凋亡。双基因联合沉默，效果更加显著。

Abstract

Objective To investigate the effect of CD105 and Ki67 silence on ovarian cancer cell line OVCAR3 cells. Methods OVCAR3 cells were transfected with siRNA of CD105-siRNA ggroup, Ki67-siRNA group, CD105-siRNA+Ki67-siRNA group, negative control groups in vitro respectively. Cell proliferation, migration, invasion and apoptosis after transfection were analysed with MTT assay, wound-healing assay, Transwell assay and flow-cytometric. Results Compared with the negative control group, the expressions of mRNA and protein in CD105-siRNA group and Ki67-siRNA group were decreased significantly;Cell proliferation, migration, invasion ability were decreased significantly after transfection; apoptosis was increased. The most significant change was detected in CD105-siRNA+Ki67-siRNA group（P<0.01,P<0.05）. Conclusion CD105-siRNA and ki67-siRNA expression vector can inhibit the proliferation of human ovarian cancer OVCAR3 cells and reduce their cell migration and invasion, and induce apoptosis in tumor cells. The effect of the double gene silence is more obvious.

导出引用

郭海荣贺帅王晓燕刘萍. 靶向沉默CD105和Ki67的表达对卵巢癌OVCAR3细胞生物学行为的影响[J]. 解剖学报. 2017, 48(4): 434-439 https://doi.org/10.16098/j.issn.0529-1356.2017.04.011

GUO Hai-rong HE Shuai WANG Xiao-yan LIU Ping. Impacts of silencing CD105 and Ki67 gene expression on the biological behavior of ovarian cancer OVCAR3 cells[J]. Acta Anatomica Sinica. 2017, 48(4): 434-439 https://doi.org/10.16098/j.issn.0529-1356.2017.04.011

参考文献

［1］Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin,2012，62(1):10-29.

［2］Feng XN, Yi TT. Expression and significance of vascular endothelial growth markers CD105 in pithelial ovrian cancer ［J］. Heilongjiang Medicine and Pharmacy, 2013, 36(6):17-18.（in Chinese）

冯晓娜，衣甜甜.血管内皮增殖标志物CD105在上皮性卵巢癌血清中的表达水平及意义［J］.黑龙江医药科学，2013,36（6）：17-18.

［3］Kuhn E,Kurman RJ,Sehdev AS,et al.Ki-67 labeling index as an adjunct in the diagnosis of serous tubal intraepithelial carcinoma ［J］.Int J Gynecol Pathol,2012,31(5):416-422.

［4］Quackenbus EJ, Letarte M. Identification of several cell surface of non-T non-B acute lymphoblastic leukemia by using monodonal antibodles［J］. J Immuno, 1985, 134(2):1276-1285.

［5］Gougos A, Letarte M. Identification of a human endothellal cell antigen with monodonal antibody 44G4 produceed against a pro-β leukemic cell line［J］. J Immuno, 1988, 141(6):1925-1933.

［6］Perez-Gomez E, Del Castillo G, Juan Francisco S, et al. The role of the TGF-beta coreceptor endoglin in cancer［J］.Scientific World Journal, 2010,10:2367-2384.

［7］Marioni G, Staffieri A, Manzato E, et al. A higher CD105-assessed microvessel density and worse prognosis in elderly patients with laryngeal carcinoma［J］.Arch Otolaryngol Head Neck Surg, 2011, 137(2):175-180.

［8］Nieman KM,Kenny HA,Penicka CV,et al.Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth［J］.Nat Med,2011,17(11):1498-1503.

［9］Vaughan S,Coward JI,Bast RC,et al.Rethinking ovarian cancer: recommendations for improving outcomes ［J］.Nat Rev Cancer,2011,11(10):719-725.

［10］Zillhardt M,Park SM,Romero IL,et al.Foretinib (GSK 1363089),an orally available multikinase inhibitor of c-Met and VEGFR-2,blocks proliferation, induces anoikis, and impairs ovarian cancer metastasis ［J］.Clin Cancer Res,2011,17(12):4042-4051.

［11］Senol EP, Tasdelen I, Adim SB, et al. A comparison of Ki67 proliferative index in primary tumor and axillary metastatic lymph nodes with length of survival in patients with breast cancer［J］. Bratisl Lek Listy, 2013, 114(11):645-649.

［12］McCall CM, Shi C, Cornish TC, et al. Grading of welldifferentiated pancreatic neuroendocrine tumors is improved by the inclusion of both Ki67 proliferative index and mitotic rate［J］. Am J Surg Pathol, 2013, 37(11):1671-1677.

［13］Heim S, Beschorner R, Mittelbronn M, et al. Increasedmitotic and pro-liferative activity are associatedwith worse prognosis in papillary tumors of thepineal region［J］.〖JP3〗Am J Surg Pathol, 2014, 38(1):106-110.

［14］Gu JX, Zhang ZhX. Relationship between CD105 and malignant tumor［J］. Contemporary Medicine, 2015, (12):16-18.(in Chinese)

顾金霞，张振新.CD105与恶性肿瘤的关系［J］.当代医学，2015,（12）：16-18.