Kr&uuml;ppel样因子4在猪脂肪间充质干细胞成脂分化过程中的表达模式

李秀 李方正 郇延军 高霞 姜忠玲 宋学雄*

doi:10.16098/j.issn.0529-1356.2017.04.008

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解剖学报 ›› 2017, Vol. 48 ›› Issue (4) : 416-420. DOI: 10.16098/j.issn.0529-1356.2017.04.008

细胞和分子生物学

Krüppel样因子4在猪脂肪间充质干细胞成脂分化过程中的表达模式

李秀李方正郇延军高霞姜忠玲宋学雄*

作者信息 +

Expression pattern of Krüppel-like factor 4 during adipogenic differentiation of porcine adipogenic mescenchymal stem cells

LI Xiu LI Fang-zheng HUAN Yan-jun GAO Xia JIANG Zhong-ling SONG Xue-xiong*

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摘要

目的探讨猪脂肪间充质干细胞（AMSCs）成脂分化过程中Krüppel样因子4（KLF4）的表达模式。方法采用F3代以上猪AMSCs进行成脂诱导分化，用油红O染色提取法检测成脂分化程度，用RT-PCR和Real-time PCR检测KLF4的表达模式。结果猪AMSCs成脂诱导分化，诱导3 d开始出现脂肪滴，诱导 10 d时成脂分化率达60%；RT-PCR检测，在诱导分化2、4、8、12和16 d时都能检测出KLF4的表达；经Real-time PCR检测，上述各诱导分化时间点的表达量分别为对照组的1.6657±0.2269、2.6790±0.0524、2.6293±0.0280、2.3633±0.1568和2.6146±0.1575倍。这些结果表明，KLF4的表达在猪AMSCs成脂诱导分化的第2天就有显著上调，表达高峰出现在成脂分化的第4天，此后持续高表达。结论在猪AMSCs成脂分化过程中，从早期到晚期，KLF4发挥持续性的调控作用。

Abstract

Objective To investigate the expression pattern of Krüppel-like factor 4 (KLF4) during adipogenic differentiation of porcine adipogenic mescenchymal stem cells (AMSCs). Methods More than F3 generation of porcine AMSCs were used during adipogenic differentiation, the level of adipogenic differentiation was detected by oil red O staining extraction assay, the expression of KLF4 was analyzed by RT-PCR and Real-time PCR. Results During the porcine AMSCs adipogenic differentiation, the lipid droplets began to appear on 3 days after induced, and the rate of adipogenic differentiation was about 60% on 10 days after induced;During the day of 2, 4, 8, 12 and 16 induced, the expression of KLF4 was detected in each detect time by RT-PCR; Quantitative analysis of the KLF4 expression levels on the above days by Real-time PCR, showed 1.6657±0.2269, 2.6790±0.0524, 2.6293±0.0280, 2.3633±0.1568 and 2.6146±0.1575 folds high compared with the uninduced control group. These results suggested that the expression of KLF4 was significantly increased on the day 2 induced, the highest expression was on the day 4 induced, and remained high expression after then. Conclusion During porcine AMSCs adipogenic differentiation, KLF4 may play a continuous regulatory role from the early to later period of induction.

导出引用

李秀李方正郇延军高霞姜忠玲宋学雄. Krüppel样因子4在猪脂肪间充质干细胞成脂分化过程中的表达模式[J]. 解剖学报. 2017, 48(4): 416-420 https://doi.org/10.16098/j.issn.0529-1356.2017.04.008

LI Xiu LI Fang-zheng HUAN Yan-jun GAO Xia JIANG Zhong-ling SONG Xue-xiong. Expression pattern of Krüppel-like factor 4 during adipogenic differentiation of porcine adipogenic mescenchymal stem cells[J]. Acta Anatomica Sinica. 2017, 48(4): 416-420 https://doi.org/10.16098/j.issn.0529-1356.2017.04.008

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